The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分別對胎鼠聽囊大学生一级A片、新生鼠聽覺上皮和成年鼠聽覺上皮用自然培養(yǎng)方法進(jìn)行體外培養(yǎng)，并應(yīng)用免疫細(xì)胞化學(xué)方法中文字幕网无码人妻精品18区，對培養(yǎng)細(xì)胞進(jìn)行染色標(biāo)記和觀察亚洲欧美综合久久久久久小说，系統(tǒng)了解哺乳動物聽覺細(xì)胞發(fā)生、發(fā)育過程爱情岛论坛首页无码免费，以及這些細(xì)胞在體外的增殖和分化過程日本一区,二区,三区AⅤ视频，旨在了解哺乳動物聽覺細(xì)胞聽覺上皮感覺細(xì)胞的來源，發(fā)現(xiàn)細(xì)胞生長的前體細(xì)胞8x成年视频在线观看免费。

Methods: The health adult human primary hepatocyte microsome was randomly divided into 12 groups for control and itraconazole with different concentrations in the range of clinical drug blood concentration, each group have 10 samples. Control groups were added culture fluid, itraconazole groups were added itraconazole with different concentration respectively, after cultured 30 minutes, substrates (phenacetin for CYP1A2, testosterone for CYP3A4) were added and cultured for another 40 minutes.

采用健康成人肝細(xì)胞微粒體狼友视频在线的，分為對照組和不同濃度的伊曲康唑組，共12組精品丝袜国产在线精品不卡，每組10個樣本精品久久久久久亚洲综合网，伊曲康唑組分別加入血藥濃度范圍內(nèi)不同對應(yīng)濃度的伊曲康唑，對照組僅加入培養(yǎng)液精品国产一级毛片在线看，孵育30 min后，再加入CYP450同工酶1A2和3A4的相應(yīng)底物再孵育40 min天天干天天透天天日。

Times as that of T. sp-1 cultured under phototrophic conditions and 3. 2 times as that of T. sp-1 cultured under heterotrophic conditions.

在兼養(yǎng)條件下客人的黄色一级片操操操，T.sp-1的生長速率達(dá)0.222 g／，分別為自養(yǎng)和異養(yǎng)培養(yǎng)條件下的1.5倍和3.2倍mianfeihuangseshipin。

When it comes to fatty acid compositions, PUFAs contents of T. sp-1 cultured in the mixotrophic and phototrophic conditions are 55. 44% and 44. 20% of total fatty acids, respectively. The contents of saturated and monosaturated fatty acids of T. sp-1 cultured under heterotrophic conditions are higher, their contens are 32. 84% and 35. 29% of total fatty acids, respectively.

在脂肪酸組成方面Aa在线免费观看网址，T.sp-1在兼養(yǎng)和自養(yǎng)條件下培養(yǎng)，脂肪酸以PUFAs為主日本精品视频在线观看，含量分別占總脂肪酸的55.44％和44.20％美女黄禁止18以下看尿口网站；而在異養(yǎng)條件下培養(yǎng)，脂肪酸以飽和脂肪酸和單飽和脂肪酸為主色色色婷婷，含量分別占總脂肪酸的32.84％和35.29％黄色视频页面入口。

Compared with the cell cultured in BG11, the cell wall of the cells cultured at low concentration of Cu2+(0.01～1 mg/L) did not markedly become thick and the chlorophyll did not change notably, but the number of pyrenoid increased.

結(jié)果表明，Cu2+對綠球藻的顯微結(jié)構(gòu)﹑生長及生理狀態(tài)的影響比較顯著原创国产。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

體外培養(yǎng)的脂肪干細(xì)胞復(fù)合于支架上日本h视频，加入誘導(dǎo)液(基礎(chǔ)培養(yǎng)液+ 50 μg/L軟骨形態(tài)發(fā)生蛋白1)繼續(xù)培養(yǎng)2周作為實驗組；將SD仔鼠軟骨細(xì)胞復(fù)合于支架上视色4色视频，常規(guī)培養(yǎng)2周作為對照組日本网站中文字幕。20只裸小鼠，左側(cè)腋窩皮下均植入實驗組細(xì)胞-支架復(fù)合物corm超碰在线，右側(cè)腋窩皮下均植入對照組細(xì)胞-支架復(fù)合物中文字字幕在线永久视频。8， 12周各處死10只永久免费观看的毛片app，行甲苯胺藍(lán)和番紅O-固綠染色欧美高清精品视频在线一区二区。

Primary cultured rat hepatocytes were cultured with various concentrations of sodium fluoride for 24 hours to study the toxicity and possible mechanism induced by fluoride from morphological, enzymological and biochemical changes.

收集細(xì)胞培養(yǎng)上清液欧洲无码一区二区，測定ALT和AST活性和MDA含量。用上述指標(biāo)來評價氟化鈉對肝細(xì)胞的毒性作用及其可能機(jī)制免费黄色视频日。

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫組化和原位雜交的檢測方法国产激情毛片，通過對體外培養(yǎng)的HEP-2喉癌細(xì)胞和803胃癌細(xì)胞和這兩株細(xì)胞在裸鼠皮下形成的實體瘤組織中bFGF，纖維細(xì)胞生長因子受體-1（fibroblast growth factor receptor-1官网啪啪啪网站，F(xiàn)GFR-1）和bFGFmRNA的檢測試圖揭示三者在體外和體內(nèi)表達(dá)以及與腫瘤形成的關(guān)系亚洲一级A片A毛，從而為這方面的研究提供更多的材料。

The experiments showed a significant increase in Bax mRNA levels (P.01) in neurons cultured at severe hypoxia condition compared with those cultured at normoxia. In hypoxia groups, tolbutamide increased Bax mRNA expression (P.05), while diazoxide reduced it (n=5 for each).

缺氧+二氮嗪組中基因Bax的mRNA表達(dá)水平與單純?nèi)毖踅M相比下降(P.01)亚洲欧美日韩专区，缺氧＋甲苯磺丁脲組中基因Bax的mRNA表達(dá)水平相對于單純?nèi)毖踅M也有提高(P.05)色播播电影。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

結(jié)果：種植于不同基質(zhì)的纖維環(huán)細(xì)胞形態(tài)學(xué)觀察無明顯差別；在兩種基質(zhì)上的纖維環(huán)細(xì)胞甲苯胺藍(lán)染色無差異国产使劲cao视频；Ⅰ草碰在线播，Ⅱ型膠原及聚集蛋白聚糖mRNA及細(xì)胞膜整合素β1的表達(dá)亦無明顯差異；纖維環(huán)細(xì)胞可以黏附于硅橡膠膜上生長欧美黑逼。