Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取從屠宰場獲取的豬卵巢中的卵母細胞国产又爽又粗又黄视频，在加入和不加hCG和PMSG的mTCMl99培養(yǎng)液中分別培養(yǎng)22小時，地衣紅染色發(fā)現77.6％的卵母細胞達到MII期；去卵丘細胞后在mTBM中用鮮精或凍精解凍后受精6小時，在NCSU23中培養(yǎng)14小時后固定染色發(fā)現受精率分別為87.5％和64.1％www7久精品九视频；電激活后在NCSU23中培養(yǎng)20小時后固定染色發(fā)現原核形成率為94.1％。

The immunocytochemistry was used to identify the RASMC.RESULTS: The RASMC were successfully cultured by the adherent method of tissue explants and grown in the "peak- valley" mode. The cultured RASMC were fusiform shape with a big oviform or round nuclei rich in cytoplasm. Immunohistochemical method ( S-P method) showed strong expression of a- smooth muscle actin.

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Methods: Between October 1999 and March 2001, 20 patients with severe acute pancreatitis following peripancreatic or peritoneal fluid collection were observed. Blood and fluid collection were dynamically cultured, at the same time, microorganisms cultured were identified, and then drug sensitivity was performed.

對1999年10月至2001年3月間收治的重癥胰腺炎伴胰周或腹腔積液的20例患者進行動態(tài)血液久久网精品aaa、腹腔積液細菌培養(yǎng)、鑒定及藥物敏感試驗国产孕妇作爱喷水视频。

Cultured for 48 hours, the floating cells were removed and the adherent cells were continued to culture with cytokines including IL-4 and GM-CSF. After 14 days culture, the induced DCs were observed by photics microscope and electron microscope, also co-cultured with native T cells derived from spleen, its stimulating function was detected by MTT assay.

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Results The strongest photoinactivation effect occured in the HEP-2 cells co-cultured with 1 mmol/L ALA for 8 h. The killing activity of ALA-PDT to the HEP-2 cells was positively correlated with the power density of the irradiation (r=0.88, P＜0.05).Conclusions ALA-PDT can effectively kill the cultured HEP-2 cells in vitro. The optimal killing activity was related to the time and the power density of the laser irradiation.

結果 (1)光照時機選擇在ALA與HEP-2細胞培養(yǎng)8 h時殺傷效應最強；(2)ALA-PDT對HEP-2細胞的殺傷效應與照光能量呈正相關r=0.88精品日韩妖精视频网站，P 結論 ALA-PDT能有效地殺傷體外培養(yǎng)的腫瘤細胞精品免费一级污片，其殺傷最佳效果與照光時機和照光能量有關。

Methods Acanthamoeba polyphaga and Legionella pneumophila were co-cultured under laboratory condition. At consecutive time points during the culture, smears of the cultured products were made on glass slides for staining purposes. Different types of stainings including Gram′s staining, Gimenez staining, Giemsa staining and immunofluorescence were used to determine the best method for the identification of amoebal pathogens.

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The results showed that, using transient GUS expression, the frequency of resistant calli and shoot differentiation as a criteria, wheat mature embryos were cultured on the preculture medium of CM4C1P for 14 days, and immersed in inoculation suspension Ⅰ for 3 hours, then the explants were co-cultured under desiccation conditions for 3 days, which was optimal conditions for Agrobacterium-mediated transformation of wheat mature embryos.

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AIM: To observe the effect of diterpenoid compound 5F of Pteris semipinnate L on apoptosis in cultured human pterygium body fibroblasts. METHODS: Fibroblasts collected from human pterygium body were cultured in vitro with 5F at different time point.

目的：觀察中藥半邊旗二萜類化合提取物5F對體外培養(yǎng)的人翼狀胬肉成纖維細胞周期的影響及5F誘導細胞凋亡過程超微結構的變化，初步探討5F對HPFs作用的機制免费A一级毛片在线播放。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

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①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

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