A 500bp fragment was amplified, and then the PCR products were digested with restriction enzym BglII.

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In this paper, PCR primers were designed based on the sequence of a single copy in atp6 gene. A 500bp fragment was amplified, and then the PCR products were digested with restriction enzym BglII.

根據(jù)atp6的基因序列設(shè)計(jì)特異擴(kuò)增引物我和子的性关系免费观看，以瓣化型胡蘿卜核質(zhì)互作雄性不育系H05A及其保持系H05B為試材AV大全在线观看麻豆，擴(kuò)增獲得了1條約500bp的特異擴(kuò)增片段。

It has been reported that NDP kinase has weak interaction with Hsp70, which can increase the ATP hydrolysis activity, so we carefully examined the possibility of NDP kinase contamination in the experiment. The equimolar mixture of NDP kinase and Hsp70 were digested and then 44-kD fragment was purified.

為獲得準(zhǔn)確的結(jié)論排除NDP激酶可能對實(shí)驗(yàn)的影響曰本特黄免费视频在线观，按照純化Hsp70 44-kD片段的方法對等物質(zhì)量NDP激酶和Hsp70混合物進(jìn)行酶切日韩性色自拍视频在线观看、純化，檢測得到的44-kD片段的ATP-ADP轉(zhuǎn)換活性日韩播放自拍视频在线观看网站免费，確定Hsp70 ATP-ADP轉(zhuǎn)換活性是Hsp70自身固有的18禁无码高潮喷水，位于其N端44-kD片段處。

Zinc deficiency in children's early performance was digested hypofunction.

小兒鋅缺乏的早期表現(xiàn)是消化功能減退8x在线视频。

In vitro digestion methods for monogastric animal were reviewed accordi ng to simulatation of sites of the digestive tract,sources of digestive enzymes and the ways for separating digested and indigested nutrients in digesta.

本文從畜禽消化道模擬部位一级a姓爱免费视频、消化酶的來源和分離已消化養(yǎng)分的方法等幾方面對單胃動(dòng)物體外消化模擬技術(shù)進(jìn)行了歸類和總結(jié)。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

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Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形蟲可誘導(dǎo)的反向重復(fù)序列RNAi載體的構(gòu)建：設(shè)計(jì)引物免费日韩黄黄色片观看品尚网，通過PCR分別擴(kuò)增弓形蟲HSP70基因5&UTR啟動(dòng)子序列(HSP70／5UTR)黄片AAAA、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因內(nèi)含子C序列，通過酶切連接国产高清无码网址，構(gòu)建以弓形蟲熱休克蛋白HSP70基因啟動(dòng)子進(jìn)行驅(qū)動(dòng)的欧美色色成，以β-微管蛋白基因內(nèi)含子C序列作為間隔序列，以HSP70基因3UTR序列作為轉(zhuǎn)錄終止信號的反向重復(fù)序列RNAi載體pBSK-HSP70／5UTR-IntronC-HSP70／3UTR先锋在线资源网站，序列測定結(jié)果正確26uuu亚洲欧美日韩在线。

Its value of BOD 5/COD fell to 0.19, moreover carbon and nitrogen was inversive, which brought much difficulty for anaerobically digested effluent to aerobically treatment.

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Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga軟件輔助下對lipA基因密碼子及RNA二級結(jié)構(gòu)進(jìn)行優(yōu)化并引入Cla I(237位)和Pst I(475位)酶切位點(diǎn)免费自拍黄色网站；通過組裝PCR分別合成lipA基因的各片段F1(237 bp)欧美欧美在线视频观看、F2(238 bp)和F3(422 bp)；通過該基因內(nèi)的Cla I和Pst I限制性酶切位點(diǎn)連接成完整的全長lipA基因视频一区无码天堂。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表達(dá)www,日本高清视频、平板裂解法和PCR擴(kuò)增三種不同方法分別對日本血吸蟲抗原cDNA基因進(jìn)行鑒定和分析，8個(gè)免疫篩選陽性克隆均能在大腸桿菌中以融合蛋白的形式表達(dá)色一区二区三区，表達(dá)蛋白分子量為140～150kDa黄色小视频网站网址在线免费观看，抗原cDNA基因經(jīng)限制性內(nèi)切酶EcoRI酶解后，瓊脂糖凝膠電泳顯示其大小為700～900bp国产A片一级黄色，PCR能擴(kuò)增出特異性條帶青青操在线视频。