The recombination vector was digested by Pac I enzyme and transfected into 293A cell by Lipofectamine method to obtain recomb...

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Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的兩端加上所需要的酶切位點(diǎn)www4438，將之連接在m／〓的3'端，構(gòu)建原核表達(dá)載體pGEX4T-1 m／〓-mTNF-α黄色视频在线播放特级a级，通過IPTG的誘導(dǎo)表達(dá)之后女人被草视频，兩種融合蛋白的表達(dá)量分別占細(xì)菌總蛋白的15％、12％91小黄片，表達(dá)產(chǎn)物經(jīng)包涵體的純化→變性→復(fù)性→純化→凝血酶酶切→進(jìn)一步純化后被操高潮了视频，可以得到純度為電泳純的m／〓-mTNF-α抗肝癌雙功能抗體。

The sample was digested by microwave,magnesium,zinc,iron in the Silurus lanzhouensis Chen were determined using flame atomic absorption spectrometry.

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METHODS: Fetal mouse liver was sterilely obtained, combed using cell scrape, and digested in type IV collagenase.

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The serum selenium is first digested by microwave treatment and then chemically purified before analysis by using sulphydryl cotton. The optimum conditions were found to b as follows:(1) The sulphydryl cotton column is prewashed with 5 mol/L hydrochloric acid;(2) Se is desorbed by adding 1 mL of concentrated nitric acid and (3) the optimum amount of sulphydryl cotton is 0.14 g. This preconcentration step is required in order to remove matrix-interfering elements, such as Na, Mg, K and Ca.

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Lastly, specimens contain HPV16 DNA were digested with restricted enyzme BamHI, and done with southern transprint to hybridize with digoxigenin-labeled probe of HPV16 genome through nuclear acid hybridization. Results (1)Among 60 cervial carcinoma specimens,the HPV16 E5 positive rate is 33%(20/60) by PCR, 70%(42/60)、58%（35/60）by nuclear acid dot hybridization with HPV16 E5 probe and HPV16 probe .

結(jié)果（1）PCR法 60例宮頸癌組織中HPV16 E5 片段的陽(yáng)性率為33%（20/60）激情亚洲综合网；（2）斑點(diǎn)雜交法60例宮頸癌組織中HPV16 E5陽(yáng)性率為70%(42/60)欧美乱伦电影一区二区，HPV16陽(yáng)性率為58%（35/60）；（3）宮頸癌組織中HPV16 E5基因的突變率為40%（8/20）黄片一区pon，遷移位置不甚一致亚洲天堂网a，隨機(jī)抽取一例E5變異的PCR陽(yáng)性產(chǎn)物進(jìn)行測(cè)序分析，結(jié)果發(fā)現(xiàn)在E5片段中有2處變異亚洲色图综合区， nt3979A→C顛換久久一级无码A片，nt3988T→C轉(zhuǎn)換，導(dǎo)致E5編碼區(qū)出現(xiàn)1個(gè)氨基酸的有義變異99爱在线精品免费观看。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

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Methods Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

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Methods utricular sensory epithelia of postnatal day 1 rats were isolated and digested by thermolysin, then were transferred to a solution containing 0.125% trypsin and 0.125% collagenase for 8 min at 37℃ to harvest the pure utricular sensory epithelial cells.

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The fresh dewater digested sludge of municipal wastewater treatment plant was suited for vaccinal sludge at the startup of anaerobic reactor and the concentration should not be less 10mg/L; the COD of feed wastewater should be lower than 5000mg/L, and VOL was 20 percent of total VOL; the alkalinity of effluent should be more than 3000mg/L; on the condition of the COD removed 80% can the VOL be elevated.

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