Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分離純化非洲爪蟾孵化酶時(shí)黄色稳定资源视频在线观看，得到了60kD和40kD兩種分子三级三级久久三级久久6，用孵化酶的特異性抗GST-UVS.2抗體進(jìn)行Western雜交的結(jié)果證明二者均為孵化酶分子午夜免费福利视频。60kD分子很不穩(wěn)定国产精华Av在线导航网站，在純化過程中極易降解最近中文字幕免费大全下载，40kD分子可能是由60kD分子經(jīng)過降解或自身降解丟失了兩個(gè)CUB重復(fù)區(qū)而形成的无码3p，而CUB重復(fù)區(qū)很可能在對受精膜分子結(jié)構(gòu)的修飾或改造中具有重要作用。

And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在啟動子兩端有人工設(shè)計(jì)合成的HindⅢ和BamHⅠ酶切位點(diǎn)91免费视频网，經(jīng)上述雙酶切消化后亚洲色黄hAV在线，回收目的基因片段與線狀載體的粘性接頭恰好匹配在线观看的黄色网站欧美日本。

Those carbohydrates not digested to monosaccharides by human enzymes in the small intestine may be metabolized by microorganisms in the large intestine, producing substances that are absorbed and catabolized for energy.

那些不能被人體小腸中的酶消化至單糖的會被大腸中微生物代謝，產(chǎn)生能被吸收和用于能量代謝的物質(zhì)日本中文字幕系列网址。

Those carbohydrates not digested to monosaccharides by human enzymes in the small intestine may be metabolized by microorganisms in the large intestine, producing substances that are absorbed and catabolized for energy.

無知的人開導(dǎo)更無知的人人人操狠狠插。7。那些不能被人體小腸中的酶消化至單糖的會被大腸中微生物代謝国产激情A∨在线视频播放，產(chǎn)生能被吸收和用于能量代謝的物質(zhì)韩国三级国产精品。

Restriction endonuclease digested genomic DNA was circularized by self-ligation, and complete gene of α subunit and β subunit was obtained by inverse PCR.

以環(huán)化的限制性內(nèi)切酶消化的基因組片段為模板，用反向PCR的方法擴(kuò)增国内A级毛片在线视频、克隆并測定了α亞基和β亞基的完整基因序列国内精品免费视频在线播放。

At the same time, the purified soil DNA was partially digested by enzyme Sau3AI, and the 2-6kb and 6-9kb fragments were recovered by dialyzing respectively.

純化后的土壤DNA用限制性內(nèi)切酶Sau3AI隨機(jī)切割，以透析袋分別回收2-6kb和6-9kb的酶切片段一级黄片欧美操逼，然后用T_4連接酶與上述脫磷載體連接国产一级A爱大片免费视频-百度。

The crude DNA pellets were recovered by dialyzing and then could be used for molecular operation such as amplification of 16S rRNA gene and digestion of restriction enzymes.The plasmid pUC19 was prepared and digested with BamHI.

粗提DNA用半透膜透析回收純化，純化后的DNA可以進(jìn)行16sRNA基因PCR擴(kuò)增欧美性爱天天干，酶切等分子生物學(xué)操作八戒成人影院午夜在线观看，具有較高的質(zhì)量。

They are suckled for two months, but at the end of that time they are able to eat half-digested flesh disgorged for them by their dam or even their sire.

他們是哺乳兩個(gè)月99久久无码，但在去年底日韩综艺午夜在线看免费，當(dāng)時(shí)他們都能夠吃半消化肉disgorged為他們自己的大壩或什至他們的妻妾。

Some books are to be tasted,others to be swallowed,and some few to be c hewed and digested.

一些書可以品味操一操a片，另外一些書可以吞食，少數(shù)的一些可以咀嚼姐久久综合、消化大大一级黄色网站。

Genomic DNA of maize was digested with restriction enzymes and hybridized with a mF2KP cDNA fragment.

應(yīng)用不同的限制性內(nèi)切酶消化玉米基因組DNA，以〓p標(biāo)記的mF2KP cDNA片段作為探針進(jìn)行Southern雜交分析午夜激爽免费毛片。