The efferent projections of hypothalamic and brain stem nucleuses to nucleus dorsalis nervi vagi were studied by means of the retrograde axonal transport of HRP technique in cats.

本文用HRP軸突逆行輸送法，對貓下丘腦和腦干一些核向迷走神經(jīng)背核的傳出投射進(jìn)行了研究成年女人一级毛片免费看。將HRP注射至迷走神經(jīng)背核后高清免费91黄色视频日韩A级片，較多的標(biāo)記細(xì)胞見于同側(cè)下丘腦的室旁核、背內(nèi)側(cè)核以及中腦的中央灰質(zhì)和被蓋腹內(nèi)側(cè)區(qū)。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

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After injecting HRP into the HVc, anterogradely labelled fibers and terminals were observed in the robust nucleus of the archistriatum, tractus archistriatum dorsalis and the area X of lobus parolfactorius.

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But the mechanism is not clear. Morphological studies have showed that medial vestibular nucleus receive the projection of serotoninergic fiber coming from nucleus raphes magus, nucleus raphes obscurus, nucleus raphes pallidus, nucleus raphes dorsalis and express the 5-HT1A, 5-HT 1B, 5-HT 2A, 5-HT 2C receptor.

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