In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本課題中欧美性交一级大片，在第一部分實(shí)驗(yàn)中采用以CD133為標(biāo)志的免疫磁珠法從人腦膠質(zhì)瘤組織和細(xì)胞株中分離腦膠質(zhì)瘤干細(xì)胞并進(jìn)行體外培養(yǎng)，通過免疫熒光技術(shù)檢測(cè)干細(xì)胞標(biāo)志物CD133亚洲色图欧美在线、Nestin欧美精品v在线视频，誘導(dǎo)分化后檢查分化細(xì)胞標(biāo)志物MAP2、GFAP毛片在线视频免费观看下载大全、MBP以及電鏡超微結(jié)構(gòu)觀察和移植SCID鼠致瘤實(shí)驗(yàn)iGAO视频为爱寻找激情swag，對(duì)其干細(xì)胞特性加以鑒定，得到如下結(jié)果：不同病理分級(jí)的新鮮膠質(zhì)瘤標(biāo)本和膠質(zhì)瘤細(xì)胞株中存在一小部分CD133+的膠質(zhì)瘤細(xì)胞国产日韩区，能表達(dá)干細(xì)胞的標(biāo)志物CD133和Nestin草久草久，符合干細(xì)胞的超微結(jié)構(gòu)特點(diǎn)，體外培養(yǎng)能連續(xù)傳代三轮美女毛片美女毛片美女；具有多向分化潛能：誘導(dǎo)分化后能產(chǎn)生MAP2iGao视频入口、β-TubulinⅢ、GFAP日本超碰在线免费观看一级、MBP染色陽性的細(xì)胞女人16一毛片；移植SCID鼠后能形成與親本腫瘤表型一致的移植瘤。