Analyzing the change of DH, PCL and active calcium, and selecting each of best method. The results:(1) Dealing with the bone under high pressure ①High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;②Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ①High content of calcium: neutral enzyme, 1500u/L, 6h;②High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

結(jié)果表明：A、高壓①骨粉及骨湯蛋白質(zhì)含量最高：壓力為0.05MPa妓女精品视频在线播放，高壓2.0h，骨水比為1：2；②骨粉中氨態(tài)氮最低：壓力0.13MPa欧美三级网站在线免费观看，高壓4.0h，骨水比為1：3超碰公开在线久；骨湯中氨態(tài)氮最低：0.10MPa亚洲h版在线，3.0h，骨水比1：4黄色视频图片一区；B国产AV一区网站、酶解①游離鈣含量最高：中性蛋白酶，酶濃度1500u/L色色射射色，酶解6h一级做a午爰片久久毛片A片午；②水解度最高：胰蛋白酶，酶濃度1：100欧美大黑吊性交，酶解6h精品爱99视频在线；③PCL值最低：胰蛋白酶，酶濃度1：125激情区小说区图片区另类，酶解6h黄片免费观看无码视频；PCL值最高：堿性蛋白酶，酶濃度1500u/L色哟哟自拍一区二区，酶解8h免费爱爱网址。

The results:(1) Dealing with the bone under high pressure ① High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;② Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ① High content of calcium: neutral enzyme, 1500u/L, 6h;② High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

結(jié)果表明： A 、高壓①骨粉及骨湯蛋白質(zhì)含量最高：壓力為0.05MPa 国产福利影院在线观看，高壓2.0h 国产国际精品福利色噜噜，骨水比為1：2；②骨粉中氨態(tài)氮最低：壓力0.13MPa 私人18禁午夜福利网站，高壓4.0h 高清黄色网址在线观看，骨水比為1：3；骨湯中氨態(tài)氮最低：0.10MPa 亚洲黄片电影免费在线观看，3.0h 十八禁止网址，骨水比1：4激情国产Av做激情国产爱； B 、酶解①游離鈣含量最高：中性蛋白酶欧美一级黄片直播，酶濃度1500u/L 欧美18xxxx，酶解6h ；②水解度最高：胰蛋白酶夜夜草AV，酶濃度1：100色色色色免费，酶解6h ；③ PCL 值最低：胰蛋白酶免费的黄色视频91，酶濃度1：125国产人人操人人摸富，酶解6h ； PCL 值最高：堿性蛋白酶国产一级免费在线黄色视频，酶濃度1500u/L 性福利网站，酶解8h 。

The result of study showed:1 The effect of some inorganic urea inhibitor enzyme indicated boracic acid inhibits the activeness of urea enzyme in soil well, delayed the speed of urea hydrolysis in the soil and decreased the volatilization of ammonia , boracic acid,can be chosen as a inorganic urea enzyme inhibitor.2 The effect of some organic urea inhibitor enzyme showed that the dissociative Humic Acids of turfy soil and weathered coal inhibit the activeness of urea enzyme in soil well.

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The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,300 IU/g of KDN pectinase,35℃、6 h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 500 IU/g of TZ-888 compound enzyme,45 ℃,6 h.

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The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on dry ramie degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,20 IU/g of KDN pectinase,4℃、4h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 300 IU/g of TZ-888 compound enzyme,40 ℃,5 h.

摘 要：本研究根據(jù)苧麻膠質(zhì)的主要成分為果膠野花社区大全免费观看3超碰、半纖維素和木質(zhì)素国产精品区在线，用高活性的KDN果膠酶和TZ-888復(fù)合酶（主要是木聚糖酶和甘露聚糖酶）從pH值、浴比久久夜色精品、酶用量欧美精品-精品国内自产拍在线、金屬離子、溫度国产又黄又湿又色的免费网站、脫膠時(shí)間等方面進(jìn)行單因素試驗(yàn)欧洲无码在线免费，然后選取影響顯著的浴比、酶用量欧美老妇交乱视频69式、溫度亚洲日韩视频第一页，時(shí)間進(jìn)行四因素三水平的脫膠試驗(yàn)，結(jié)果表明干苧麻采用KDN果膠酶脫膠最佳組合為浴比1：12欧美熟线视频免费精品，pH 8.6久久综合精品一区无码， 1 mmoL/L Mg2+，酶用量200 IU/g亚日日日日日视频，溫度45 ℃欧美黄色网站视频、時(shí)間4 h時(shí)處理，TZ-888復(fù)合酶脫膠最佳組為浴比1：18偷拍自偷拍，pH 4.0国产熟女大声叫受不了视频， 1 mmoL/L Ca2+，酶用量300 IU/g色哟在线视，溫度40 ℃亚洲日本网，時(shí)間5h進(jìn)行脫膠為優(yōu)化的試驗(yàn)條件。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表達(dá)載體 pET-20b 和 pET-28a，構(gòu)建重組質(zhì)粒 pET-20b-bglA 和 pET-28a-bglA，然后轉(zhuǎn)化不同大腸桿菌 E.coli 宿主日韩精品火爆视频在线观看网站，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中獲得高效表達(dá)国产精品第一页福利在线，重組蛋白的表達(dá)量為 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)无码视少妇视频一区二区三区，可溶性比例為 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)欧美大一片免费网站，比酶活達(dá) 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，這些結(jié)果表明www.爱99，E.coli BL21-CodonPlus(DE3)-RIL 宿主帶有的 argU tRNA三级片网站视频免费看、ileY tRNA 和 leuW tRNA 基因，分別增強(qiáng)對(duì)稀有密碼子 AGA/AGG精品国产看高清国产AV毛片、AUA 和 CUA 的識(shí)別亚洲国产毛片在线看，有助于提高該酶在 E.coli 中的表達(dá)；進(jìn)一步優(yōu)化誘導(dǎo)條件美女午夜大片免费视频，重組 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下誘導(dǎo)培養(yǎng)男人舔女人天堂网站，IPTG 濃度由 1000 μM 降至 25 μM，目的蛋白可溶性表達(dá)由 12.3%增至 32.8%人人摸人人操589，提高 1.7 倍黄色视频了88，16 ℃下低溫誘導(dǎo)實(shí)現(xiàn)目的蛋白 70.6%的可溶性表達(dá)，較 37 ℃下誘導(dǎo)培養(yǎng)提高 1.2 倍91P0RN专干大屁股。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①I(mǎi)n the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我們根據(jù)在脲或鹽酸胍存在下的底物反應(yīng)動(dòng)力學(xué)方程求得游離酶和酶底物二元开心激情婷婷色、三元復(fù)合物的微觀動(dòng)力學(xué)常數(shù)，結(jié)果表明：①酶-底物二元欧美性交网、三元復(fù)合物的失活速度明顯慢于游離酶欧美图片视频小说，說(shuō)明兩個(gè)底物二氫葉酸和NADPH對(duì)酶的失活都具有一定程度的保護(hù)作用；②在脲作用下先锋影音av鲁色资源站，酶的失活和構(gòu)象變化均為單指數(shù)項(xiàng)過(guò)程爱爱午夜福利视频，而且酶的活力喪失和三級(jí)結(jié)構(gòu)變化是同時(shí)發(fā)生的，說(shuō)明二氫葉酸還原酶的脲變性可能是一個(gè)"全或無(wú)"的兩態(tài)過(guò)程欧美熟女性高潮视频；③在鹽酸胍作用下，酶的構(gòu)象變化為兩相過(guò)程天堂AV综合久久天堂，而失活則是單指數(shù)項(xiàng)過(guò)程黄色视频在线三区，酶分子構(gòu)象變化的快相速度常數(shù)與失活速度常數(shù)基本一致，因此我們認(rèn)為二氫葉酸還原酶的鹽酸胍變性過(guò)程中存在一個(gè)沒(méi)有活力免费.黄色.视频.网址、但仍具備一定空間結(jié)構(gòu)的變性中間體内射亚洲美女视频在线搞。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P＜0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P＜0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P＞0.05). Enzymepreparation significantly increased (P＜0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P＞0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

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Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

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Furthermore, the effects of different metallic salts,nitrogen sources,carbon sources,temperature,pH,water content and fermentation time on the enzyme activity and the yield of the acid protease were studied and the results were as follows: both MnCl2 and CuSO4 could activate the protease while other metallic salts inhibited the protease on different levels; addition of 1 % bean flour and 1 % glucose could increase enzyme yield and enzyme activity by 71 % and 31 % respectively; the maximal yield of enzyme was achieved under the conditions of temperature at 40 ℃ and pH as 7.0; water content and fermentation time would also influence enzyme yield on different levels.

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