The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后射射射干干，研究固定化酶降解甲基對(duì)硫磷，從不同材料的膜載體固定有機(jī)磷水解酶的比較中国产专区欧美精品激情，發(fā)現(xiàn)親水膜比疏水膜適合作固定化酶載體饥渴老熟妇女人乱伦视频；在此基礎(chǔ)上，將有機(jī)磷水解酶固定在聚醚砜微濾膜上黄色视频中文字幕，并制成酶膜生物反應(yīng)器学生妹网站，用于降解甲基對(duì)硫磷；戊二醛化學(xué)交聯(lián)法固定時(shí)欧美日一级大片，酶液與10%牛血清白蛋白溶液的比例在3：1~2：1亚洲欧美性爱视频日韩字幕，與交聯(lián)劑的比例在7：2~7：4范圍內(nèi)，固定化效果較好在线看成年人视频，并發(fā)現(xiàn)牛血清白蛋白和膜載體有降低酶活損失的作用久草新日韩；將固定化的酶膜裝于酶膜反應(yīng)器降解甲基對(duì)硫磷，實(shí)驗(yàn)結(jié)果表明：反應(yīng)器的降解速率與固定化酶量呈正比免费在线综合精品观看，也隨底物濃度的增加而加快av免费体验区试看，當(dāng)流速低時(shí)，降解速率隨流速增大而加快亚洲av人妻不卡，到7ml/min以上時(shí)www黄视频在线观看，降解速率不隨流速腦黽傭洌到獾淖畹團(tuán)ǘ任？

The results indicates that enzyme combination diluent is suited for the production of HCV antibody enzyme linked immunosorbent diagnostic reagents,if the enzyme combination diluent regards PBST as buffer liquid and BSA and Casein irrelated albumen as stability .

結(jié)論以PBST為緩沖液黄色性爱视频亚洲可以看的，以BSA和C as ein無關(guān)蛋白作穩(wěn)定劑組成酶結(jié)合物稀釋液適用于HCV抗體酶聯(lián)免疫診斷試劑的生產(chǎn)视频A黄。

In this study, we investigate the heat-induced inhibition of drug-metabolizing enzyme activity in mice fed with freeze-dried, heated freeze-dried or half amount autolyzed freeze-dried Brussels sprouts powder. 0.5% ethoxyquin diet was fed to another group of mice as a positive control. The study mice, four per group, were fed a diet consisting of 20% Brussels sprouts for 7 days. Each group had its own pair-fed group. We found significantly greater inductions of glutathione S- transferase, quinine reductase, and phase Ⅱ drug-metabolizing enzyme activities in groups fed within freeze-dried and heated freeze-dried Brussels sprouts than their pair-fed groups. These findings indicate that intestinal microflora still can hydrolyze glucosinolates without plant myrosinase activity, though the alteration of cytochrome- P-450 1A (CYP1A, a phase Ⅰ drug-metabolizing enzyme) induction was only 1.4-fold.

本實(shí)驗(yàn)欲進(jìn)一步探討加熱處理之抱子甘藍(lán)對(duì)phase Ⅰ與phase Ⅱ解毒酵素活性之影響差異，實(shí)驗(yàn)分三組免费a片视频网站，抱子甘藍(lán)(1)冷凍乾燥組（glucosinolate未裂解）(2)加熱冷凍乾燥組(3)半量之裂解冷凍乾燥組（glucosinolate已裂解）2022中文字幕在线观看，(4)and 0.5% ethoxyquin diet was used as a positive control以2%重量混合成飼料，餵以小白鼠（4只／組）7天国产毛片精品全部视频，結(jié)果發(fā)現(xiàn)一级黄片免费看视频，在第(2)組小白鼠GST phase Ⅱ酵素）活性顯著上升，顯示在植物裂解酵素不存在之狀況下欧美性XXXXX极品视频，腸胃道之微生物仍可促進(jìn)裂解反應(yīng)疯狂伦交成人网站视频在线观看，但由CYP1A活性增加不顯著（僅1.4倍）看來，根據(jù)定義：?jiǎn)为?dú)phase Ⅱ酵素活性增加国产免费丝袜激情视频，為monofunctional induction/inducer官方免费av网站，可加速致癌物之代謝人人搞人人操超碰91。

Compared with thermozyme,the first-class structure change of the psychrophilic enzyme can lead to space structure change and the structure of the enzyme is more flexible.Thus,this enzyme can catalyze with less energy in low temperature.

與嗜溫酶相比，嗜冷酶一級(jí)結(jié)構(gòu)的變化導(dǎo)致空間結(jié)構(gòu)的變化国产精a成品人v在线播放，使酶分子的構(gòu)象具有更大的柔性国产免费的黄网站在线观看视频，同時(shí)也有利于這類酶在低溫條件下需要更少的能量而發(fā)揮催化作用。

Study on 6 soil enzyme activities and 7 siol fertility factors of 12 different planting modes,analyse the dates with the correlation analysis and the canonical correalation analysis,In order to reveal the relations between the soil enzyme activities and soil fertilities in the low hill lands along Yantze River , The result of the correlation analysis showed that they had a great relation between the soil enzyme activities and siol fertility factors.

對(duì)12種不同種植模式的6個(gè)土壤酶活性和7個(gè)土壤肥力因子進(jìn)行測(cè)定福利大片，運(yùn)用相關(guān)分析和典型相關(guān)分析方法對(duì)數(shù)據(jù)進(jìn)行分析经典在线视频日韩精品免费，以期闡明沿江丘陵地區(qū)土壤酶活性與土壤肥力因子的關(guān)系。相關(guān)分析的結(jié)果表明土壤酶活性與土壤肥力因子密切相關(guān)污污污污污www免费网站，多數(shù)呈極顯著正相關(guān)九九插美女视频。

Studied the stachydrine dealing with the Oncomelania hupensis's esterase、the glycogen and the total protein. The result showed when dealt with the Oncomelania hupensis for 1~2 days, the figures of enzyme are higher than the compared group; when dealt for 3~4 days, the figures of enzyme reached the highest level; when dealt for 5 days, the figures of enzyme remarkably reduced.

用益母草中水蘇堿處理釘螺導(dǎo)致其酯酶同工酶www.av小日本、糖原及總蛋白變化的實(shí)驗(yàn)結(jié)果表明：處理1~2d后釘螺的酶活高于清水對(duì)照組最新精品黄色网站久爱，處理3~4d后釘螺的酶活最強(qiáng)，而處理5d后釘螺樣品的酶活則大大減弱二区农村乱沦视频。

The pH stability of enzyme was 8 and the temperature stability of enzyme was 30℃. Bap-degrading enzyme catalyzed the oxidation of veratryl alcohol to veratraldehyde without H2O2 and the oxidation of Mn2+ by H2O2 to Mn3+.

酵素在將veratryl alcohol氧化生成veratraldehyde時(shí)是不需要H2O2協(xié)助催化亚洲综合日韩Av无码毛片天堂，然而在對(duì)Mn2+離子氧化生成Mn3+離子時(shí)若有H2O2協(xié)助催化下可以加快酵素催化的速度。

Enzyme technology has been used in oil and fats research for years, such as enzyme degumming for oils, enzyme modification for lecithins and biodiesel productions.

酶反應(yīng)技術(shù)在油脂研究中已經(jīng)得到了一定的應(yīng)用亚洲福利一区黑人，如酶法脫膠精品久久中文字幕久久av、磷脂酶法改性、生物燃料的研究等等亚洲中文久久精品无码国产精品。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus亚洲综合综合网，F(xiàn)MDV)China99株結(jié)構(gòu)蛋白基因及多個(gè)非結(jié)構(gòu)蛋白基因的DNA疫苗，本研究通過定點(diǎn)突變方法和PCR擴(kuò)增方法亚洲视频在线免费公开，獲得包含有FMDV China99株結(jié)構(gòu)蛋白P118禁毛片永久免费观看、非結(jié)構(gòu)蛋白2A、3C以及部分2B久久八视频、3B編碼基因的片段P12X3C和包含有FMDV China99株結(jié)構(gòu)蛋白P1、非結(jié)構(gòu)蛋白2A午夜性生活网站、3C偷拍性爱网站、3D以及部分2B、3B編碼基因的片段P12X3C3D黑人性爱黄片，將獲得的基因片段直接/酶切后與同樣處理的真核表達(dá)質(zhì)粒連接超碰国产人人操，分別得到重組質(zhì)粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D免费看黄色片视频；對(duì)重組質(zhì)粒進(jìn)行序列測(cè)定日本精品久久、分析，并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細(xì)胞草久草网，通過雙抗體夾心ELISA方法和間接免疫熒光標(biāo)記方法檢測(cè)細(xì)胞中FMDV抗原的表達(dá)国产免费AA视频，用電子顯微鏡觀察病毒空衣殼的組裝；為評(píng)價(jià)重組質(zhì)粒作為DNA疫苗對(duì)實(shí)驗(yàn)動(dòng)物及本動(dòng)物的免疫效果，將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠乱伦色一区二区，并與酵母表達(dá)的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達(dá)質(zhì)粒pcDNA3.1/IFN分別/同時(shí)免疫香港经典a毛片久久免费观看2，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；隨后將質(zhì)粒pcDNA3.1/P12X3C98超碰人人与人人欧美、pcDNA3.1/P12X3C3D與帶有牛α干擾素的真核表達(dá)質(zhì)粒pcDNA3.1/IFN同時(shí)免疫牛先锋久久中文，三周后經(jīng)牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus偷自拍另类亚洲清纯唯美，F(xiàn)MDV)China99株結(jié)構(gòu)蛋白基因及多個(gè)非結(jié)構(gòu)蛋白基因的DNA疫曲爱爱视频在哪里考，本研究通過定點(diǎn)突變方法和PCR擴(kuò)增方法，獲得包含有FMDV China99株結(jié)構(gòu)蛋白P1久久性生活视频免费、非結(jié)構(gòu)蛋白2A黑人一级婬片A片免费无码、3C以及部分2B、3B編碼基因的片段P12X3C和包含有FMDV China99株結(jié)構(gòu)蛋白P1欧美性交农夫导航、非結(jié)構(gòu)蛋白2A欧美日韩国产无线码一区、3C、3D以及部分2B国模视频、3B編碼基因的片段P12X3C3D性无码专区一级，將獲得的基因片段直接／酶切后與同樣處理的真核表達(dá)質(zhì)粒連接，分別得到重組質(zhì)粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D国产免费性生活视频、pTARGET／P12X3C3D又色又爽又黄的黄网站18禁；對(duì)重組質(zhì)粒進(jìn)行序列測(cè)定、分析欧美日韩操，并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細(xì)胞欧美日韩国产啊啊啊，通過雙抗體夾心ELISA方法和間接免疫熒光標(biāo)記方法檢測(cè)細(xì)胞中FMDV抗原的表達(dá)，用電子顯微鏡觀察病毒空衣殼的組裝来部一级毛片；為評(píng)價(jià)重組質(zhì)粒作為DNA疫苗對(duì)實(shí)驗(yàn)動(dòng)物及本動(dòng)物的免疫效果91在线精彩，將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠，并與酵母表達(dá)的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達(dá)質(zhì)粒pcDNA3.1／IFN分別／同時(shí)免疫国产普通话对白精品视频，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：隨后將質(zhì)粒pcDNA3.1／P12X3C亚洲最新的黄色网站、pcDNA3.1／P12X3C3D與帶有牛α干擾素的真核表達(dá)質(zhì)粒pcDNA3.1／IFN同時(shí)免疫牛，三周后經(jīng)牛舌皮攻以10~4ID_(50)的O型FMDV China99株A片免费首页在线观看。