Through different plant growth regulator of the combination of different observation explant adventitious buds of the induction, elongation and rooting results.

通過不同植物生長調(diào)節(jié)物的配合，觀察不同外植體不定芽的誘導(dǎo)、伸長和生根的效果。

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

構(gòu)建了兩個基因的pBI121和pCAMBIA1300植物表達(dá)載體免费A片大大片，并通過農(nóng)桿菌將上述基因轉(zhuǎn)入煙草和擬南芥中，得到了卡那抗性的再生植株2018精品黄色视频。4榴莲视频在线下载地址ios、以中國水仙不同部位外植體進(jìn)行了不定芽直接分化和愈傷組織誘導(dǎo)分化試驗欧美A级在线现免费观看，結(jié)果表明一级毛片不卡免费，雙鱗片外植體在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+瓊脂7.5 g/L培養(yǎng)基上可以大量地直接再生，再生芽數(shù)量平均可達(dá)13~16個/cm雙鱗片狼友网站国产，比傳統(tǒng)的鱗莖盤之間分化提高30~50%色呦呦.m3u8；鱗片、葉片亚洲伊人蕉综合网、花葶及花部不同組織在適宜的配方中均可不同程度地誘導(dǎo)出愈傷組織日韩午夜在线试看网站视频免费，但以帶花藥的花絲誘導(dǎo)出的愈傷組織最好。

Methods Explant culture of human nasal epithelial cells. Ciliary beat frequency was measured by digital high-speed microscope.

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Result］ The new buds and petals of I.germanica was proper to be used as explant of tissue culture, with lower browning rate compared with other positions. The well situated tissue block of explant (3 mm×3 mm×3 mm) might reduce browning rate. Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning. The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning, and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning. When using disinfectants of alcohol, its dipping time of 5～10 s for explant was the best, with the minimum browning rate. The culture under full illumination condition was liable to induce brownness turning, compared with other cultured conditions, the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.

結(jié)果］ 鳶尾的新生芽和花瓣較適宜作組織培養(yǎng)的外植體，與其他部位相比褐變率較低五月婷综合色色色色色；外植體組織塊適中（3 mm×3 mm×3 mm）可降低褐變率亚洲欧美黄色录像在线观看；在培養(yǎng)基中加入0.1%Vc或0.3%活性炭對防止組織褐化效果明顯；外源激素2 mg/L 6-BA易引發(fā)褐變国产精品视频人人做人人，2 mg/L Kt和2 mg/L NAA對組織褐變影響不大日日操人人干9197视频；使用乙醇消毒劑時，對外植體浸泡時間以5～10 s為佳2021精品手机在线，褐變率最低亚洲26UUU；全光照條件培養(yǎng)容易誘發(fā)褐變，采用仿人工氣候條件培養(yǎng)較其他條件培養(yǎng)可有效抑制褐變的發(fā)生一级黄色毛片视频免费看。

0Mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.

結(jié)果表明：不同黃瓜品種直接不定芽數(shù)量誘導(dǎo)存在顯著差異国产91精品专区，但長度誘導(dǎo)無顯著差異人人操日韩超碰；6-芐氨基嘌呤對直接不定芽數(shù)量和長度的誘導(dǎo)作用顯著，其中4.0mg/L為數(shù)量誘導(dǎo)適宜濃度欧美亚洲图片小说，其出芽率和每外植體出芽數(shù)達(dá)到最高日韩第一视频，分別為100.0%和11.1，0.5mg/L為長度誘導(dǎo)適宜濃度在线你你懂，其平均芽長12.0mm色爱综合区国产精品；外植體的大小對直接不定芽數(shù)量和長度均具顯著影響，隨著子葉的增大誘導(dǎo)直接不定芽的數(shù)量和長度增加欧美性爱白人性69交性，其中單片完整子葉為最適宜大小黄色视频在线观看链接，其出芽率、每外植體出芽數(shù)和平均芽長分別達(dá)到100%学生妹网站、6.7和17.1mm欧美黑白激情性爱；硝酸銀也對直接不定芽數(shù)量誘導(dǎo)影響顯著，其中2.0mg/L為適宜濃度亚洲欧美性爱视频日韩字幕，出芽率和每外植體出芽數(shù)分別達(dá)到97.2%和4.2www.99riav113.com，但對芽長有抑制作用。

The stem apex of Hemerocallis fulva was taken as explant cultured in vitro to induce callus with different culture medium. The effects of different concentration 6-BA and NAA culture medium on explant growth was studied.

采取大花萱草莖尖為外植體接種在不同培養(yǎng)基上久草新日韩，研究了不同濃度的NAA和6-BA對于外植體生長的影響抠逼黄片15.0，并探索了最適于產(chǎn)生愈傷組織和生根的培養(yǎng)基配方。

The explant are rhizome and leaf of Pyrola calliantha It has the best result by using 0.1% HgCl_2 for 7min for the explant of rhizome, for 5min for the explant of leaf.

對外植體的消毒方法已經(jīng)掌握av免费体验区试看，用0.1%的升汞對鹿蹄草莖段的滅菌采用7min最佳9reav，用0.1%的升汞對鹿蹄草葉片的滅菌采用5min最佳。

Concolor as the explant, the effects of different sterilizing times and mediums on the explant tissue culture process were studied.

方法］以野生渥丹不同部位的鱗片為外植體www黄视频在线观看，研究不同滅菌時間人人插人人操人人看、不同培養(yǎng)基對外植體組織培養(yǎng)過程的影響。

The best explant in the culture was brown leaf,and the explant sterilized in 0.1% aqueous mercuric chloride for 5min has the best effect;At the initial stage in induction,3% glucosan have the best effect following with 1% glucosan+2%sucrose are optimum in the process of culturing;the optimum culture medium is 1/2MS; the preferable medium of callus is 1/2MS+ KT(0.1mg/L)+6-BA(0.05mg/L)+2.4-D(0.4mg/l);differentiation1/2MS+6-BA(0.3mg/l)+NAA0.2+KT(0.05mg/l);and the preferable medium of rooting is :1/2MS+NAA(0.05mg/L).

安祖花組織培養(yǎng)的最佳外植體為棕色葉片视频A黄；最佳消毒時間為0.1%的升汞消毒5min91原创国产视频；最佳碳源：誘導(dǎo)初期3%的葡萄糖的誘導(dǎo)效果最佳，后期培養(yǎng)以2%蔗糖+1%葡萄糖的培養(yǎng)效果較好2022中文字幕在线观看；最佳基本培養(yǎng)基為1/2MS黄色三级网站在线观看不卡。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

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