The stem segments of Cerasus humilis Bunge with axillary bud were used as explant in this experiment and the natural regenaration plants were obtained in vitro culture of explants.

本試驗(yàn)以歐李當(dāng)年生嫩枝上帶有腋芽的莖段為外植體進(jìn)行離體組織培養(yǎng)国产熟视频在线，獲得了正常的再生植株99久久99这里只有免费精品01。

Tue explant sources played an important role in in ducement of embryogenic callus, there are differences between varities.

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Statistic analysis indicated that the main factor which influenced callus induction and the inducting rate of fasciculated shoots were explant, the optimal medium for callus induction was MS+1.0mg/L BA+1.0mg/L NAA+0.5mg/L 2,4-D+Leaves.

論文摘要：采用正交設(shè)計(jì)方法国产性爱三级免费小视频，研究了6-BA国内乱视频在线观看、NAA、2国产三级电影网站，4-D及外植體4種不同因素及其組合對鐘花櫻愈傷組織及叢生芽誘導(dǎo)率的影響黄色视频91在线，試驗(yàn)結(jié)果統(tǒng)計(jì)分析表明：外植體是誘導(dǎo)愈傷組織和誘導(dǎo)叢生芽的最主要因素。

The ligamentous scaffold material were made from the PVA fibers by braiding technique. The mechanical properties of the scaffold materials were measured using the electronic materials testing machine. Human ACL cells were isolated and cultured by tissue explant and collagenase digestion methods, the morphous characteristics of cells and the secretion of collagen protein were measured. In order to observe the biocompatibility between the material and cells, ACL cells were in vitro expanded and seeded on the scaffold materials.

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After having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time,incubation time and co-culture time,we set up the system of high frequency transformation of tomato cotyledons.Then hBMP-3m gene was transferred into tomato via Agrobacterium-mQdiated transformation,and the resistant plants to Hyg were obtained.

建立了以番茄子葉為外植體的遺傳轉(zhuǎn)化體系欧美日韩在线观看综合，確定了預(yù)培養(yǎng)時(shí)間、浸菌時(shí)間及共培養(yǎng)時(shí)間等轉(zhuǎn)化條件秘书高跟黑色丝袜国产在线，建立了番茄高頻轉(zhuǎn)化系統(tǒng)自拍偷拍1，然后通過農(nóng)桿菌介導(dǎo)法將hBMP-3m基因?qū)敕眩⑶耀@得了Hyg抗性植株18禁动漫黄片。

The results showed that 7-day-precultured embryogenic calli had the best responses for bombardment among 2-7 day' preculture duration. Enhanced transformation efficiency was found in the explants treated with high osmotic medium 4h prior to till 24 h after bombardment. Particles of gold and tungsten were both found suitable for rice transformation and there was no adverse effect when tungsten particles were reduced to 1/3 standard amount for DNA precipitation on transient GUS gene expression. However, it was found that gold particle had higher GUS expression at 120 h post-bombardment. A preliminary microprojectile transformation system with an average of 15-20 blue spots/explant GUS expression was routinely obtained for 'Tainung 67' by using the particle gun with 1,100 psi rupture disk, 6 or 9 cm microcarrier flight distance and 4 mm of macrocarrier flight distance setting.

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Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

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IDO inhibits the proliferation of eukaryotic intracellular pathogens or tumor cells by depriving them of tryptophan. IDO has been targeted for neurological disorders: it causes decrease of serotonin level in the brain resulting depression, and it causes the accumulation of neurotoxic quinolinic acid. IDO plays a key role in the suppression of T cell response and antitumor immune, it induces immune tolerance to embryo and explant.

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Adopt optimized transformation system of the melon cultivar: the explant infected in the engineering bacterium the OD560 of which was 0.30 for 15min, and co-cultured for 3days; Choosing cultivated in MS +1mg/L 6 - BA + 500mg/L carb + 75mg/L Kan, extend cultivated in MS +0.05mg/L 6 - BA + 300mg/L carb + 75mg/L Kan and rhizogenic cultivated in 1/2MS + 200mg/L cef + 25mg/L Kan.

對甜瓜栽培品種的轉(zhuǎn)化采用已優(yōu)化好的轉(zhuǎn)化體系：農(nóng)桿菌菌液OD560=0.3，侵染時(shí)間為15min黄色网站在线观看不卡，外植體選用近軸端子葉26uuu.com；共培養(yǎng)時(shí)間為72h。

The results showed that pre-treatment and antibiotic marinating for 5 h and suffocating for 12 min as well as ordinary disinfection for 12 min for surface disinfection could effectively control explant contamination. the contaminate rate with this method would be less than 20%.

結(jié)果表明：采用抗菌素浸泡5 h在线观看AV站网免费观看、熏蒸12 min并配合常規(guī)消毒處理(75%酒精浸泡1 min日韩Av无码精品人妻系列；3.0%～5.0%的次氯酸鈉和0.1%升汞1∶1混合溶液浸泡10～12 min)對外植體進(jìn)行綜合滅菌，能較快地殺滅竹芋外植體表面細(xì)菌久在线草视频，使初次接種污染率控制在20%以下国产一区二区视频www乱伦。