The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌農(nóng)桿菌介導(dǎo)的芪合酶基因遺傳轉(zhuǎn)化的最佳條件為：將預(yù)培養(yǎng)2 d的煙草外植體男人的天堂综合网，用稀釋10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培養(yǎng)3 d日韩3级电影网，然后轉(zhuǎn)入含卡那霉素50 mg/L和羧芐青霉素500 mg/L的篩選分化培養(yǎng)基上誘導(dǎo)分化国产视频33区，待抗性芽長到2～3 cm后日韩av一级免费在线观看网站，轉(zhuǎn)入含Km 50 mg/L和Cb 500 mg/L的篩選生根培養(yǎng)基上進(jìn)行篩選，在以上最佳遺傳轉(zhuǎn)化條件下欧美成αⅴ人在线视频，初步篩選到54株轉(zhuǎn)化體www热色com，經(jīng)PCR和RT-PCR檢測獲得25株轉(zhuǎn)化煙草植株。

The three day old seedlings grown in medium supplemented with 5 mg/L 6-BA were used to prepare the cotyledonary-node explants. The cotyledonary-node explants had one cotyledon and half of the embryonic axis.

選用在高達(dá)5 mg/L 6-芐氨基嘌呤(6-BA)的種子萌發(fā)培養(yǎng)基上生長3 d的蒺藜苜蓿幼苗AV野狼，制備獲得由1片子葉和半個(gè)胚軸組成的子葉節(jié)外植體欧美大黑屄。

This paper reports something about it .The callus was induced and proliferated by using young stem , leaf and leafstalk as explants .which came out in early spring . In this paper , its effects on induction and proliferation were discussed by using different kinds of explants and hormone and different physical and chemical factors.The growth circle and some physiological and biochemical factors were studied during the culture of callus, a set of rapid propagation techniques were founded, which was rapid, efficient and steady.

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Cultured in MS medium with different concentration of calcium, explants of Luffa Cylindrica, which could not generate root in usual medium, generated roots with enhanced rates and amounts according to the concentration of supplemented calcium. Moreover, some explants show transformed root from either shoot or flower bud.

在正常Ca2+濃度下色欲aⅤ，僅激素處理不能生根的絲瓜外植體第1页欧美高清视频看片在线观看网站，處以不同Ca2+濃度處理時(shí)，隨Ca2+濃度升高www.玖玖爱免费视频，外植體生根數(shù)明顯加快并增多毛片总合网，且有少量植株葉腋處異化生根，在一定程度上改變了腋芽的發(fā)育方向china农村野外妇女BBw，這說明Ca2+改變了卷須芽或花芽的發(fā)育方向男插女激情免费网址在线观看，使其發(fā)育為根。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本實(shí)驗(yàn)以菊花的莖尖為外植體国产无套推油按摩女视频推油，研究了不同滅菌方法国产日韩欧美妖亚欧在线，光照強(qiáng)度，全光照在在线黄色视频、半光照和弱光處理以及不同6-BA和NAA濃度及配比對于試管苗增殖的影響日本sy一级黄色片，并研究了珍珠巖、蛭石和河砂等瓊脂替代物對于菊花生根的影響肏屄视频网站，結(jié)果表明：在初代培養(yǎng)物建立的過程中采用0.1％升汞進(jìn)行表面滅菌的效果好于飽和漂白粉的滅菌效果成一级在线视频；在全光照條件下外植體的增殖倍數(shù)最高；在試管苗增殖培養(yǎng)的過程中，以MS為基本培養(yǎng)基鲁一鲁在线观看精品视频，并在培養(yǎng)基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA在线黄色视频了,，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最適于外植體的分化和增殖，其分化率為100％无码又黄又湿又免费视频下载，增殖倍數(shù)為12免費看男女高潮又猛又爽。

On the media containing 0.02 and 0.04 g/L potassium sorbate, the survival rates of explants were 13% and 3% resp. On the other media, all the explants died.

在含0.02和0.04 g/L山梨酸鉀的培養(yǎng)基上外植體的存活率分別為13%和3%，其余培養(yǎng)基上的外植體全部死亡99精品热视频只有精品16。

Cardiac explants grew well in the collagen gel. Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.

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Agrobacterium tumefaciens-mediated transformation system of Medicago truncatula cotyledonary-node explants Abstract The three-day-old seedlings of Medicago truncatula, which geminated in medium containing up to 5mg/L 6-BA, were used to prepare the cotyledonary-node explants which contained one cotyledon and 1-2mm of split hypocotyl. High-efficiency regeneration system of Medicago truncarula was developed by axillary shoot organogenesis of cotyledonary-node explants.

論文第二部分 Medicago truncatula遺傳轉(zhuǎn)化和植株再生體系的研究第一章根癌農(nóng)桿菌介導(dǎo)的Medicago truncatula子葉節(jié)外植體轉(zhuǎn)化系統(tǒng)的研究本文利用在高達(dá)5mg／L 6-BA的萌發(fā)培養(yǎng)基上生長3天的Medicago truncatula幼苗，制備獲得由一片子葉和1-2mm切分的胚軸組成的子葉節(jié)外植體久久精品房事视频。

Bulb scale segments of Lilium longiflorum were used as original explants and shoot sections excised from shoot clusters arosed from the first explants as the secondary explants.

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Results For callus induction of leaf explants of C. orchioides, dark treatment gave better results compared to light treatment; among the media tested, the suitable phytohormone combinations were 2.0 mg/L 2, 4-D or 6-BA 1.5 mg/L 2, 4-D 2.5 mg/L, and 300 mg/L CH 0.2% AC was good for plantlet regeneration from leaf explants. The callus from leaf explants mainly originated from midrib. The parenchyma cells near epicuticle of midrib firstly were initiated to division. Then the parenchyma cells of vascular bundle sheath and mesophyll cells on each side of vascular bundle were also divided to form callus.

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