Results showed that the frequencies of shoot regeneration of cotyledons explants from 4 d old in vitro grown seedlings were markedly increased from 34% to 46% via a preculture procedure of 2 days period using the media containing the hormone combinations of 2,4 D 0.5-1.2 g/L and 6 BA 0.2 mg/L.

結(jié)果表明黄页网站免费观看大全，苗齡4d的幼苗子葉于含2初黄色视频在线免费观看，4－D0.5－1.2mg／L和6－BA0.2mg／L的培養(yǎng)基培養(yǎng)2或6d后再轉(zhuǎn)到分化培養(yǎng)基上培養(yǎng)免费在线观看一级黄色视频网址免费在线观看，芽再生率提高34％－46％亚洲免费va在线播放。

For L. barbarum transformation, the optimized procedure was using 3-week-old leaf explants, preculture for 3-4 days, infection with 1∶1 dilution of an overnight culture of EHA101 (pIG121Hm) suspension with AS and co-culture for 3 days at 24℃ in the dark.

通過農(nóng)桿菌轉(zhuǎn)化條件的優(yōu)化你懂得在线网站，將AS活化的過夜培養(yǎng)EHA101（pIG121Hm）菌液稀釋一倍后感染預(yù)培養(yǎng)3—4天的三周齡葉外植體亚色91，并在24℃黑暗條件下共培養(yǎng)3天，可以獲得較為理想的轉(zhuǎn)化效率（62.5％）国产拍线一区观看。

The results Showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 2 d, the explants were in fected 3 min with 50 mg/L acetosyringone and OD600=0.5 Agrobacterium culture liquid, and then co-cultivated for 2 d. Resistant shoots from leaf disks were screened on MS0.4 mg/L NAA + 100 mg/L Cef medium supplemented with 10 mg/L Km.

結(jié)果表明：2 d的預(yù)培養(yǎng)亚洲美女黄色，OD600值約為0.5的農(nóng)桿菌菌液添加50 mg/L AS，侵染時間3 min人人操人人草人人爱，2 d的共培養(yǎng)黑人乱伦，從分化培養(yǎng)基獲得的抗性芽在生根培養(yǎng)基MS+0.4 mg/L NAA+10 mg/L Km+100 mg/L Cef上進(jìn)行延遲篩選，有利于獲得較高的轉(zhuǎn)化效率人人干人人插视频。

The best time for preculture was 3d. The explants treated with sonication for 20-30 seconds before co-cultivating with Agrobacterium achieved the highest PCR positive frequency.

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The tested parameters included: the effect of preculture duration for explants, the type of microcarriers, density of microcarrier particles used for DNA preparation, the effects of osmotic treatments, and plasmids with various constructions. Parameters of biolistic device including the flight distance of microcarriers (6 and 9 cm) and macrocarriers (4, 8, and 12 mm) were investigated.

評估之因子包括：培植體預(yù)培養(yǎng)天數(shù)α片一区、微載體種類及用量、高滲透壓處理及轉(zhuǎn)殖質(zhì)體對轉(zhuǎn)殖之影響一级A片高潮喷免费观看；基因鎗測試之條件有：微載體至射擊目標(biāo)之飛行距離(6永久免费AV网站可以直接看的、9 cm)，大載片至擋片之距離（4、8及12 mm）思思欧美另类自拍。

Cotyledon explants culture ; preculture procedure ; high frequency shoot regeneration ; Brasscia napus ; Brassica campestris

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The results showed that, using transient GUS expression, the frequency of resistant calli and shoot differentiation as a criteria, wheat mature embryos were cultured on the preculture medium of CM4C1P for 14 days, and immersed in inoculation suspension Ⅰ for 3 hours, then the explants were co-cultured under desiccation conditions for 3 days, which was optimal conditions for Agrobacterium-mediated transformation of wheat mature embryos.

為了進(jìn)一步完善根癌農(nóng)桿菌介導(dǎo)的小麥遺傳轉(zhuǎn)化體系，以小麥成熟胚為轉(zhuǎn)化受體顶级大樵片，研究了根癌農(nóng)桿菌介導(dǎo)法轉(zhuǎn)化小麥的主要影響因素婷婷六月丁香综合，結(jié)果表明，以gus基因瞬時表達(dá)率亚洲欧洲av官网、抗性愈傷組織獲得率和分化率為轉(zhuǎn)化指標(biāo)国产欧美日韩操逼视频，小麥成熟胚在預(yù)培養(yǎng)基CM4C1P上預(yù)培養(yǎng)14d后，用侵染培養(yǎng)液Ⅰ侵染3h4444免费，再經(jīng)干燥共培養(yǎng)方式處理3d色色色色色色女内，是根癌農(nóng)桿菌介導(dǎo)成熟胚轉(zhuǎn)化的合適條件。

The results showed that 7-day-precultured embryogenic calli had the best responses for bombardment among 2-7 day' preculture duration. Enhanced transformation efficiency was found in the explants treated with high osmotic medium 4h prior to till 24 h after bombardment. Particles of gold and tungsten were both found suitable for rice transformation and there was no adverse effect when tungsten particles were reduced to 1/3 standard amount for DNA precipitation on transient GUS gene expression. However, it was found that gold particle had higher GUS expression at 120 h post-bombardment. A preliminary microprojectile transformation system with an average of 15-20 blue spots/explant GUS expression was routinely obtained for 'Tainung 67' by using the particle gun with 1,100 psi rupture disk, 6 or 9 cm microcarrier flight distance and 4 mm of macrocarrier flight distance setting.

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The results show that the single-layered neural tube fails to form and the neural crest cells remain at the lateral regions in the explants with NTDs. However, the patterning of the neural tube is not affected as indicated by the normal expression of the preneural genes.

結(jié)果顯示神經(jīng)管閉合缺陷的移植體不能形成單層化的神經(jīng)管亚洲国产精品激情一区二区，并且神經(jīng)晴細(xì)胞滯留在側(cè)面區(qū)域不能向背側(cè)中線遷移人人操人人爱97，而對神經(jīng)前體標(biāo)記基因的檢測顯示神經(jīng)管的背腹圖式形成并未受到影響。

In explants of W5, DNA and RNA content increased in both ovules and parenchyma. DNAcontent in proligerous cells decreased after 6 days of culture. Amylose increased inparenchyma gradually and big starch grains appeared in proligerous cells.

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