The first demonstration of the formation of adventive emtryos from carrot tissue explants can be traced to the work of Reinert.

由胡羅卜組織外植體來(lái)的可定胚形成的最初證實(shí)可追溯到Reinert的工作瑟网站。

The half-seed method system of Agrobacterium-mediated soybean transformation was researched applying healthy explants rates, resistant tufty shoots and elongated shoots as targets. Explants of soybean grow well in environments with abundant light and oxygen in cocultivation.

以農(nóng)桿菌介導(dǎo)的半種法大豆遺傳轉(zhuǎn)化系統(tǒng)為基礎(chǔ)黑人A片特级又粗又大，對(duì)目的基因進(jìn)行轉(zhuǎn)化五月草视频；以健康外植體獲得率激情文学综合区图片区小说区、抗性叢生芽獲得率和抗性芽伸長(zhǎng)比率為指標(biāo)，對(duì)轉(zhuǎn)化系統(tǒng)條件進(jìn)行優(yōu)化91色色视屏。

Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.

在三維膠原中，心臟組織塊生長(zhǎng)良好亚洲鲁在视频在线观看，遷出的細(xì)胞將相鄰組織塊連接起來(lái)AAA亚洲无码高清，組織塊有自發(fā)的搏動(dòng)。

Well developed shoots were successfully obtained after 45 days of culture of shoot tip explants on MS medium supplemented with 4-8 mg/l Benzylaminopurine and 0.5 mg/l α-Naphthaleneacetic acid. Addition of 0.01-0.5 mg/l Thidiazuron in the medium was found to be beneficial for rapid growth of shoot tip explants.

培植體培養(yǎng)於MS基本鹽類(lèi)并添加4-8mg/l BA和0.5mg/l NAA的培養(yǎng)基45天后俄国av在线播放，對(duì)促進(jìn)新莖節(jié)萌發(fā)效果良好一级免费SV黄片；而接種於含0.01-0.5mg/l TDZ固體培養(yǎng)之莖頂培植體，也顯示有促進(jìn)莖節(jié)快速生長(zhǎng)的作用日本一本之道高清不卡免。

The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

結(jié)果表明：草毒葉片外植體對(duì)卡那霉素較為敏感www..cao，適宜的篩選濃度為20mg/L，可明顯抑制非轉(zhuǎn)化組織的生長(zhǎng)男人的天堂最新网址；300mg/L的羧吩青霉素可有效抑菌人人上人人操超碰，對(duì)外植體生長(zhǎng)影響也較腥巳嗽弧；預(yù)培養(yǎng)2-4d有利于轉(zhuǎn)化欧美乱交牲交乱频；共培養(yǎng)2-3d有利于提高轉(zhuǎn)化頻率并避免了農(nóng)桿菌的過(guò)度生長(zhǎng)黄页网在线观看免费视频；OD(下標(biāo) 600nm)值為0.4的菌液侵染10min效果最佳。

Orexin A(100 and 1000 nM) stimulated the release of GnRH from hypothalamic explants harvested from prepuberal swine.However,only 1000 nM orexin B could stimulated the release of GnRH from the hypothalamic explants harvested from prepuberal swine.

五国产拍精品、Orexin對(duì)性成熟青年母豬下丘腦組織-垂體-卵巢軸作用的體外研究為探討orexin在生殖調(diào)節(jié)中直接作用的靶位點(diǎn)男女互插在线观看，我們?cè)隗w外培養(yǎng)了豬下丘腦腦片、垂體和卵巢細(xì)胞人人摸日日干，研究了orexin A和orexin B在體外對(duì)下丘腦欧美日韩久荜、腺垂體和卵巢GnRH、LH和E2釋放的影響国产免费一级二级AV。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin組合濃度處理者国产wwwWwW性性，具有顯著降低污染率的效果分別為60%及50%；克服內(nèi)生菌污染而建立初代無(wú)菌培養(yǎng)之腋芽培植體欧美黄色性爱综合网A片，則以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin處理者91av超碰在线观看，芽體再生率最高70%-75%，惟平均每一腋芽培植體芽體增殖數(shù)僅1.2-1.4個(gè)亚州一级毛片干带视频；另在醣種類(lèi)及濃度與抗生素試驗(yàn)毛片啪啪片A片，顯示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素濃度組合添加至含3g/L半乳糖的培養(yǎng)基，顯著降低污染率至20%黄网免费观看在线网站；但未污染之培植體黄页网站免视频9，形態(tài)發(fā)生潛能低至40%以?xún)?nèi)，可反應(yīng)生長(zhǎng)之培植體芽體形成數(shù)也僅達(dá)1-1.2個(gè)之間亚洲免费福利视频，再生芽體之生長(zhǎng)也顯現(xiàn)抑制現(xiàn)象日欧美女人。

The small bulbs induced were full and the shapes of them were regular. The explants incubating in other seasons were not suitable. By comparison different incision methods, we found it' was better taking stem plate carrying bulb leaves as explants than any other treatments.

通過(guò)對(duì)不同季節(jié)的水仙鱗莖外植體進(jìn)行組培觀察，發(fā)現(xiàn)8月份至次年5月份接種的水仙外植體欧美午夜艳，小鱗莖的誘導(dǎo)率幾乎達(dá)到100%五月丁香综合在线视频；并且，小鱗莖比較飽滿亚洲三级片视频，形態(tài)比較規(guī)則caoporen永久免费公开视频，其它時(shí)間接種的水仙外植體的組培效果均不理想。

The calli from two explants of three genotypes have shownsignificant difference in regeneration.Genotypic variationin culture response was also significant.The maximumregeneration was achieved in Basmati 198 followed by Basmati 370and KS 282 in both explants.

三個(gè)基因型的兩種不同外植體的愈傷組織表現(xiàn)出顯著不同的再生能力一区二区强奸，基因型的差異對(duì)組織培養(yǎng)的差異也很明顯色噜噜网，Basmati 198在繼Basmati 370和KS 282的兩種外植體之后達(dá)到7最大的再生能力。

The tissue culture and rapid propagation of Mozzie buster were studied by using tips and leaves as explants.The results showed that: The optimum disinfect time for the explants with Hgcl2 was 5~7 min.

以蚊凈香草的葉片和莖段作為外植體欧美一级俄罗斯a片，用0.1%的升汞作為消毒劑中文字字幕在线播放，通過(guò)試驗(yàn)擬找出最佳的消毒時(shí)間及適于外植體的誘導(dǎo)、增殖和生根的培養(yǎng)基。