Objective: To study the proper medium for tissue c ulture of the explants from Hypericum perforatum.

目的：探索貫葉連翹外植體組織培養(yǎng)的適合培養(yǎng)基配方。

Its hypocotyl and leaf of cotyledon were better explants for inducing calli on the MS medium with 0.1mg/L NAA .

幼苗的下胚軸和子葉葉片在MS+0.1mg／L NAA的培養(yǎng)基上可以獲得較好的愈傷組織无码精品日韩网站视频。

With the leaf and leafstalk of S. miltiorrhiza Bge. f. alba as explants, the different medium were used to induce clustered buds, strengthen regenerative bud and conduct rooting induction so as to establish a set of rapid propagation system.

方法］以白花丹參的幼嫩葉片和葉柄作為外植體欧美老妓女视频，接種至器官發(fā)生分化培養(yǎng)基上誘導(dǎo)叢生芽美女的私密秘网站，然后作壯苗培養(yǎng)日韩制服丝袜无码网站视频，繼而生根培養(yǎng)日韩视频在线一区第四页，建立一套快速再生體系。

The leaves, leafstalk, and stems from grapes were used as explants to induce calli and subculture in medium (100 mL B5 medium containing 3 g sucrose and 0.6 g agar) with different plant hormone combinations (6-BA, KT, IBA, 2, 4-D), and then the resveratol content from those calli was determinated by using HPLC.

以葡萄的葉黄色A片区全部免费、葉柄和莖分別作為外植體国产区精品第一页，在不同激素組合（6-卞氨基嘌呤(6-BA)、激動素、吲哚丁酸亚洲性爱一级、2，4-二氯苯氧乙酸(2欧美最爽的乱婬视频婬色A片，4-D)）的培養(yǎng)基（以B5培養(yǎng)基為基本培養(yǎng)基另类亚洲精品网站av，含30g/L蔗糖和6g/L瓊脂）上，進行愈傷組織的誘導(dǎo)和繼代培養(yǎng)在线欧美视频网址www，并利用高效液相色譜法對不同來源愈傷組織中的白藜蘆醇含量進行測定欧美黄频免费a。

About calli from different explants, there were significant differences in their resveratol content, and their medium of the highest content expressed were respectively: for leaves, B5 medium+6-BA 0.5 mg/L+IBA 0.2 mg/L; for leafstalk, B5 medium+6-BA 1.0 mg/L+IBA 0.2 mg/L; for stems, B5 medium+6-BA 0.5 mg/L+IBA 0.2 mg/L.

以葉、葉柄和莖為外植體誘導(dǎo)愈傷組織時成年人的视频在线观看，其白藜蘆醇含量最高的培養(yǎng)基分別為B5培養(yǎng)基+6-BA0.5mg/L+IBA0.2mg/L影音先锋综合网站、B5培養(yǎng)基+6-BA1.0mg/L+IBA0.2mg/L和B5培養(yǎng)基+6-BA0.5mg/L+IBA0.2mg/L。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

體細胞胚胎發(fā)生研究的結(jié)果表明：(1)成熟過程中的合子胚是誘導(dǎo)水曲柳體細胞胚胎發(fā)生的最佳外植體材料最黄欧美性爱黄图；(2)在所試驗到的MS日韩美女自拍视频宅男、MS1/2（將MS的所有成分均減半）、WPMwww.的黄色网站、DKW免费毛片A一级在线播放、B〓、SH等六種基本培養(yǎng)基中国产黄色网站免费在现观看，MS1/2是最適合誘導(dǎo)水曲柳體細胞胚胎發(fā)生的基本培養(yǎng)基国内一级婬片A片免费久久免费；(3)水曲柳的體細胞胚胎發(fā)生以直接發(fā)生為主，體細胞胚主要來自于從合子胚分離的完整子葉欧美一级特黄大片做受在线观看s；(4)培養(yǎng)基中的激素組合對水曲柳的體細胞胚胎發(fā)生有顯著影響日韩美女久久久无吗，誘導(dǎo)直接體細胞胚發(fā)生較好的激素組合有NAA 1mg/L+IBA 2，5婷婷操操b，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0疯狂欧美黑粗硬大插进去了，2mg/L+TDZ 0.05mg/L；(5)合子胚分離的下胚軸具有最強的愈傷組織誘導(dǎo)潛力激情另类小说，少數(shù)愈傷組織可以分化出體細胞胚欧美性爱人人操天天干；(6)愈傷組織的誘導(dǎo)也受培養(yǎng)基和激素配比的顯著影響，最適宜誘導(dǎo)的培養(yǎng)條件為MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2女生叉开腿让男生桶，5mg/L+TDZ0.05mg/L欧美A级中文字幕乱片；(7)采種時間對體細胞胚胎發(fā)生有顯著影響。7月末到8月中旬的合子胚具有較強的體細胞胚發(fā)生潛力欧美黑人巨大巨粗性XXXX，此時種子尚未成熟欧美另类69高清XXXXX，胚乳已呈固態(tài)，種胚的各個部分已分化完全看AV片网站，種胚體積占胚腔的大約2/3在线视频A∨；(8)在各自綜合的最適條件下，完整子葉的體細胞胚誘導(dǎo)率可達33.68%，下胚軸的愈傷組織誘導(dǎo)率可達90.7%欧美日韩www。

A regeneration system was established from hypocotyl explants of Medico go sativa cultivar Nongbao.

對苜蓿的下胚軸外植體進行離體培養(yǎng)建立了再生體系A片在线观看免费的网站。

The explants sterilized with 0.1% mercuric chloride for 5 min grew normally and their contamination rate was only 3.3%.

用0.1% HgCl2滅菌5 min的外植體生長正常，污染率僅為3.3%国内永久第一免费福利视频。

Efficient callus induction and regeneration procedure were established using growing tip of octoploid triticale 'h739' at glume differentiation stage as explants.Embryonic somaclones were selected.

利用八倍體小黑麥'h739'護穎分化期幼穗作外植體亚洲精品愉拍偷拍网址，成功地建立了愈傷組織誘導(dǎo)和再生程序，并獲得了長期保持胚性的無性系色战五月天天天天天。

Lateral shoots of Oncidium"Honey"were used as explants for tissue culture. The basic media, hormone combinations, pH, sucrose concentration and additives on protocorm proliferation were compared to develop techniques for raising strong plantlets and roots, transplanting, and hardening off.

以文心蘭盆栽品種'蜜糖'的側(cè)芽為實驗材料狼友视频在线观看的，研究基本培養(yǎng)基、激素配比欧美人牲交免费黄色一级片爱情就爱、pH值欧洲亚洲一级毛片一区二区高清视频、蔗糖濃度和添加物等對原球莖增殖的影響，探討文心蘭的生根壯苗與煉苗移栽技術(shù)国产一级婬片A片免费无码。