Selected the best cultivate condition by cultivated on four different media under different conditions; Observated morphology perperties; Studied on biology perperties by germ tube testing, Carbohydrate fermentation testing, Tween testing and Esculin dissociation; lysis testing.

選擇四種不同的培養(yǎng)基分別在不同溫度條件下培養(yǎng)，選出最優(yōu)培養(yǎng)條件；通過(guò)光學(xué)顯微鏡、電子顯微鏡觀察菌落及菌體的形態(tài)學(xué)特點(diǎn)；通過(guò)芽管試驗(yàn)三级片手机免费看中文视频、碳水化合物發(fā)酵試驗(yàn)、碳水化合物同化試驗(yàn)18紧网站、沙堡培養(yǎng)基生長(zhǎng)試驗(yàn)欧美黄色午夜、過(guò)氧化氫酶試驗(yàn)、七葉苷分解試驗(yàn)无遮无码免费裸交视频暖暖、吐溫試驗(yàn)研究該菌的生理色色黄片、生化學(xué)特征；應(yīng)用PCR技術(shù)通過(guò)特異性的引物擴(kuò)增得到菌屬特異性的片段精精国产XXXX视频在线my，最后測(cè)定擴(kuò)增片段的核苷酸序列美女下面网站，從而進(jìn)行菌種的分析、鑒定538免费精品视频。

Methods: The time of primany fermentation of the negtive fermentation tube which is neither aicd nor gas was prolonged from 1824 h which. Is stipulated by nation criterion detection of coliform bacteria to 3648 h and 358 samples of 7 categories were investigated.

依據(jù)國(guó)標(biāo)法檢測(cè)大腸菌群數(shù)国产一国产一级毛片视频在线，將初發(fā)酵培養(yǎng)18~24 h未產(chǎn)酸產(chǎn)氣的陰性管樣品繼續(xù)培養(yǎng)至36~48 h，對(duì)7類358份樣品進(jìn)行大腸菌群數(shù)的檢測(cè)日日摸日日碰日日添夜夜爽。

B order to make full use of Ta1, it is essential to improve the investigation to industrial production scale by drawing assistance from high cell density fermentation. Pilot test of tube culture and flask shaking culture were done to get optimized culture conditions. Finally, we reached the goal of high cell density fermentation and high level expression of recombinant human Ta1 E.

在經(jīng)過(guò)試管以及三角搖瓶水平對(duì)培養(yǎng)條件進(jìn)行摸索后野外免费视频毛片，我們成功地實(shí)現(xiàn)了該工程菌的高密度發(fā)酵，3乙菌液獲得濕菌體工4口丁蛋白表達(dá)水平與試管水平相當(dāng)五月丁香六月综合欧美在线观看。

MUG single tube quantitative assay method was introduced to Escherichia coli assay for bacterial suspensions,natural seawater,and fishery product samples.The detected data was analyzed and compared with multiple tube fermentation method.

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The results showed: no significant difference was found between the results of single tube quantitative assay method and multiple tube fermentation technique;single tube quantitative assay method showed better accurateness,precision,and reproducibility than multiple tube fermentation technique;single tube quantitative assay method was quicker and more economical.

結(jié)果表明：MUG單管定量檢測(cè)法與大腸埃希氏菌多管發(fā)酵法對(duì)菌懸液www.手机无码视频、天然海水综合久久本道中文字幕www、水產(chǎn)品的檢測(cè)結(jié)果沒(méi)有統(tǒng)計(jì)學(xué)差異；MUG單管定量檢測(cè)法檢測(cè)數(shù)據(jù)的精確性高于大腸埃希氏菌多管發(fā)酵法美女裸体网站18禁免费看，而且更為快速中文字幕日韩专区最新、經(jīng)濟(jì)。

This experiment on the fermentation of multiple water samples for coliform determination of three phases: the beginning of the fermentation test, flat panel to verify the separation and recovery fermentation, fermentation of the final restoration coliform test there exist, then in accordance with the beginning of the fermentation tube tests positive the greatest possible number of investigations the number of coliforms coliform report the number of keys.

本實(shí)驗(yàn)中闡述的多管發(fā)酵法對(duì)水樣中大腸菌群的測(cè)定包括三個(gè)階段：初發(fā)酵試驗(yàn)久久热首页，平板分離和復(fù)發(fā)酵驗(yàn)證国产性色AV网址无毒不卡，經(jīng)最后復(fù)發(fā)酵試驗(yàn)驗(yàn)證實(shí)有大腸菌群存在后，再根據(jù)初發(fā)酵試驗(yàn)的陽(yáng)性管數(shù)查大腸菌群最大可能數(shù)檢索表報(bào)告大腸菌群數(shù)。

One L-isoleucine high-producing strain ISW330 with genetic marker was screened through shake tube primary screening, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation This strain could produce L-isoleucine 9.18g/L after fermentation under the fermentation condition without being optimized.

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One L-glutamic acid high-producing strain TN6319 with genetic maker (FEA~r+KMA~r+SG~r ) was screened through shake tube primary screening ,shake flask second screening,genetic marker test and single clone isolation and continuous passage cultibation.this strain could produce L-glutamic 72.9g/L after fermentation 42h under the fermentation without being optimized.

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The main products: large acid-base tank, stainless steel fermentation tank, concentrated liquor cans, seeds, cans, nitrogen tank, condensate, water, automatic air pressure to water tanks, Jinqi cans, vacuum furnace, distillation columns, vacuum tower, the reactor , Condenser, deaerator, air filters, buffers, water collection and bellows heat exchanger, U-tube heat exchanger, such as titanium tetrachloride heater.

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