The result indicated that 237 combinations amplified a total of 13 079 AFLP fragments with an average of 55 fragments, the average of 1.30 markers per 1 000 AFLP fragments were found to be linked to clubroot resistant gene; the amplified polymorphic fragments were 3 167 between the two parents, account for 24 percent of polymorphic frequency; the number of amplified fragments and the number of polymorphic fragments was in positive linear correlation; GC content of AFLP primer combinations significantly affects the number of amplified fragments and the number of polymorphic fragments, indicating negative linear correlation between them.

結果表明：平均每個組合檢測到55條帶中文字幕高清不卡超碰，共擴增13079條帶毛级黄片视频XXXX，平均每1000條帶檢測到1.30個根腫病抗性基因連鎖標記；多態(tài)性帶數(shù)為3167條欧美性交黄色一级片，雙親間多態(tài)性頻率約為0.24国产青榴视频网；擴增帶數(shù)與多態(tài)性帶數(shù)呈線性正相關，引物組合中的不同GC含量與擴增帶數(shù)和多態(tài)性帶數(shù)存在顯著性差異九一在线国内精品视频，并表現(xiàn)出線性負相關久久毛片免费全部播放无码。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

結果發(fā)現(xiàn)2個在胚芽和胚根中表達量都很高的cDNA片段代表的是編碼玉米β-D-葡糖苷酶的基因；一個在胚芽中表達而在胚根中不表達的片段代表的是編碼線粒體分子伴侶HSP60的基因久久riav25.co；片段MFl2的大部分克隆測序結果是與葉綠體基因組中編碼核糖體小亞基蛋白prp22的基因同源亚洲A∨无码乱码在线观着性色，但其中有一個克隆測定的cDNA片段序列推測的氨基酸序列與CDC5家族成員有較高的同源性；片段MF17的大部分克隆測序結果與葉綠體基因組中編碼核糖體蛋白prp19的基因同源国内精品视频久久，而有一克隆測定的cDNA片段序列推測的氨基酸序列與參與信號轉導的膜結合蛋白VAP27和VAMP有較高的同源性一区二区免费视频在线观看；另有3個優(yōu)先在胚芽中表達的cDNA片段未查詢到同源序列。

According to the experiment results of degradation in field, degradation mechanism of the biodegradable mulch film can include four stages: the first, the material surface appeared deficiency while the surface area and interstice increased of material in the action of natural environment and microorganism; the second, along with the surface area and interstice of material increased and the gap strengthened, the chemical chain of the high polymer's principal chain broke as a result of the hydrolysis or the enzymatic effect in the action of hydration, causing the molecular weight and mechanics function descended; the third, the molecular of high polymer split the low polymer after its physical mechanical function descended, the material decomposed the fragments, the mass defect intensified; the fourth, the low polymer of fragments form in the natural environment, the microorganism, the biological enzyme became the smaller fragments, thus absorbed by the phagocytes, or further turn into CO2 and H2O due to hydrolysis.

根據(jù)田間降解試驗結果推測视频在线精品，生物全降解地膜的降解機理可能包括四個階段：第一日韩精品免费人成视频观看，在自然環(huán)境、微生物作用下超粉嫩000福利视频合集，材料表面出現(xiàn)缺陷92在线精品午夜视频在线观看，同時表面積增大、空隙增多黄网站在线视频免费观看；第二久久精品视频无，隨著材料表面積的增大、空隙的增多色多多艹逼干炮，吸水性增強久久久久9999国产精品，在自水合作用下，高聚物主鏈由于水解或酶解而使化學鏈斷裂午夜毛a片，導致分子量和力學性能下降日韩无码黄片免费高清；第三，在其物理機械性能下降后欧美特黄性爱视屏，高聚物分子鏈斷裂為低聚物分子黄色网址求，材料裂解為碎片狀，質量損失加勁访廊蘸缫咕贰亚洲欧洲日韩综合色一二区；第四，碎片狀低聚物在自然環(huán)境、微生物美女黄片毛片在线看、生物酶作用下進一步水解變成尺寸更小的碎片日韩精品无码一区二区三区99，從而被吞噬細胞吸收，或進一步水解日黄特一级黄色A片，生成CO2和H2O色在线观看黄视频。

Four fragments -the cDNA and DNA fragments of Lectin gene,the cDNA and DNA fragments of Pectate Lyase gene were obtained from the fruit of Musa Acuminata AAA SPP.

本研究通過RT-PCR和PCR擴增的方法，從巴西香蕉Musa Acuminata AAA国产黄A一级毛片视频。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物學軟件DNAStar對COR和BBE基因的cDNA序列同源性進行分析比較一级一级一级一级毛片，分別從各基因中篩選和克隆了一段同源性極低、約400～500 bp的片段在线免费看一级片；并應用重疊PCR法將其拼接成744 bp的融合基因BC国产三级片黑免费，以中間載體pHANNIBAL和植物表達載體pART27為基礎，構建了以CaMV 35S啟動子驅動的含有"正向BC融合片段- pdk內含子-反向BC融合片段"的ihRNAi植物表達載體久久久国产一级AV片，通過轉化野生罌粟免费视频网站嗯啊轻点视频，初步研究了以COR和BBE基因為靶標的RNAi對內源嗎啡合成的抑制效果，為進一步培育低嗎啡高蒂巴因的罌粟種質提供了依據(jù)99亚洲国产精品二产精品。

The results showed that 17 gene fragments were expressed exclusively,26 gene fragments expressed increasingly,and 20 gene fragments expressed decreasingly in incompatible combination compared with either in compatible combination or in control.

結果表明亚洲无码激情，非親和性互作中特有的表達片段7條，較親和性互作及對照表達明顯增強的片段21條最新精品国偷一区二区，表達明顯減弱的18條可在线观看网站91。

We have investigated the conformational features of four short peptide fragments of Staphylococcal nuclease by homonuclear two-dimensional NMR and circular dichroism with a view to examining the early events in the folding process of SNase.

中文題名金黃色葡萄球菌核酸酶折疊起始位點：N-末端短肽段的NMR研究副題名外文題名 Folding initiation sites of staphylococcal nuclease：exploring the solution conformation of N-terminal fragments by nuclear magnetic resonance 論文作者戴繼勛導師王金鳳研究員學科專業(yè)分子生物學研究領域\研究方向學位級別博士學位授予單位中國科學院生物物理研究所學位授予日期2001 論文頁碼總數(shù)68頁關鍵詞金黃色葡萄球菌核酸酶蛋白質折疊核磁共振館藏號BSLW /2001 /Q71 /89 本論文工作用四個金黃色葡萄球菌核酸酶N-末端短片段作為新生肽鏈折疊的模型，用同核二維核磁共振譜和遠紫外圓二色譜研究了它們的溶液構象特征未满十八禁止免费网址。

One sunny afternoon, as half the world enjoyed the beach and the other half imbibed the red-wine spritzer known as tinto de verano on various terraces across town, 42-year-old Pere Bell s discussed his own joint exhibit "Dels Fragments al Conjunt" at the Galeria Marges-U, a space run by the artists Gustavo Carb Berthold and his wife, Nobuko Kihira.

一個陽光明媚的下午黄色色色色色色色，一部分人正在享受著海灘的恩惠，另一部分人正在市郊的田園里歡度這個品嘗葡萄酒操逼污视频网站，稱作tinto de verano的節(jié)日黄页免费视频观看。四十二歲的貝爾談論了他自己的展覽。&Dels Fragments al Conjunt&在Galeria Marges-U国产一级A片手机在线观看视频，在一塊由古斯塔沃和他的妻子經(jīng)營的一塊空地上日韩情侣中文自拍视频网站。

One sunny afternoon, as half the world enjoyed the beach and the other half imbibed the red-wine spritzer known as tinto de verano on various terraces across town, 42-year-old Pere Bell鑣 discussed his own joint exhibit "Dels Fragments al Conjunt" at the Galeria Marges-U, a space run by the artists Gustavo Carb?

一個陽光明媚的下午，很多人都下到海灘上嬉戲去了91人妻最真实的绿帽，其他人散落小鎮(zhèn)各處角落的大小廣場，陶醉在范拉諾紅葡萄酒之中欧美黑粗硬大一级A。42歲的貝利-貝勒斯此時正在馬基斯-U展覽館里討論他的聯(lián)展《重拾碎片》（&Dels Fragments al Conjunt&）乱色欧美激惰午夜。

Five pairs of primers were designed for PCR according to five fragments of the putative virulent genes acquired by suppression subtractive hybridization between virulent strain HA9801 and avirulent strain 12 of Streptococcus suis type 2 （ SS2 ）. The five fragments were transcriptional regulator (15# and 154# fragments), amino acid permease (210# fragments), ABC transporter (9# fragments) and surface anchored protein (187# fragments).

通過豬鏈球菌 2 型（ Streptococcus suis type 2， SS2 ）毒力菌株 HA9801 與無毒力菌株 12#之間的抑制性差減雜交實驗日韩精品网站网址在线看视频，獲得了可能與 SS2 菌株毒力相關的 5 個基因片段色色图片综合一区，分別是轉錄調節(jié)子（ 15#，和 154#片段），氨基酸通透酶（ 210#片段）美女打开双腿扒开屁股眼让男人桶， ABC 轉運子（ 9#片段）及表面錨定蛋白（ 187#片段）欧美性逼。