The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文庫總DNA91欧美精品激情在线，用BamHⅠ和HindⅢ完全酶切電泳欧美超碰视屏，用低熔點(diǎn)瓊脂糖回收長約0.5-1.5kb的DNA片段国产毛片爽爽影院无码；連接到pUC18上欧日XX片，pUC DNA先用BamHⅠ和HindⅢ酶切亚洲欧美日韩精品视频一区，后用堿性磷酸酯酶脫磷无码国产原创日韩制服AV。

In this study, the cDNA fragments encoding α subunit of nicotinic acetylcholine receptor was cloned from the diamond back moth, Plutella xylostella with RT-PCR techniques. The amplified fragments are 425 nucleotides in length, from which 141 amino acids were deduced.

測序結(jié)果顯示，擴(kuò)增的目的基因片段包含了nAChRα亞基的三種亞型亚洲视频一,一二一，分別命名為Pxal超碰网站在线、Pxa2、Pxa3福利影院最新地址发布页，都具有典型的α亞基的結(jié)構(gòu)：兩個相鄰的半胱氨酸狠狠操美女vs亚洲；形成胞外環(huán)的15個氨基酸；與煙堿和α銀環(huán)蛇毒素結(jié)合有關(guān)的氨基酸殘基色色亚洲色图一区。

Mitochondrial 16S rRNA gene and COⅠ gene fragments were amplified and sequenced from 4 wild populations of Portunus trituberculatus. Respectively, 524bp and 658bp long partial gene fragments of the 16S rRNA gene and the COⅠ gene were obtained. A high percentage of A+T base composition in the two sequences was observed, which was similar to the results of studies on drosophila, shrimp, crab and other invertebrates.

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Methods A retrospective analysis of refixation of fractured skull bone fragments was made. From January 2001 to November 2001, we operated on 52 patients with fractured bone fragments.

2 0 0 1年 1～ 11月行開顱手術(shù)的頭部外傷患者中有 5 2例骨瓣碎裂呈多塊免费观看国产强奸视频，其中 2 4例應(yīng)用顱骨鈦鉚釘回復(fù)固定顱骨骨折片，分析其術(shù)中操作国产在线精品二区、術(shù)后臨床觀察及CT檢查等情況欧美老人黄色一级视频。

And different kinds of curves also reflected different composes of sedimentary fragments and conditions of sediment: the curve in the shape of normality acute kurtosis reflects the sedimentary condition of sand which were blown by wind; the curve in the shape of skewness broad kurtosis means fragments formed by river; and the curve in the shape of saddlelike kurtosis shows a relationship with the condition of deep water.

不同類型的曲線反映了不同的碎屑物源組成和不同的沉積環(huán)境：在該剖面正態(tài)尖峰代表風(fēng)沙物源，偏態(tài)寬峰代表河水物源亚洲一黄级，正態(tài)尖峰和偏態(tài)寬峰的疊加代表風(fēng)沙物源與河水物源的混合国产缴情久久久久影院老熟女，鞍狀寬峰可能與深水環(huán)境有關(guān)。

Results:Fractures were demonstrated definitely with transaxial imaging,SSD and MPR in all patients.The length,shape,direction and displacement of fractures,the appearance,size and space location of detached bone fragments,and dislocation of joint were displayed stereoscopically by SSD.Joint disruption and detached bone fragments within joint could be best viewed by MPR.

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To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重疊延伸PCR方法將三個毒素基因国产精品日韩在线观看最大，即副溶血性弧菌的耐熱型直接溶血素基因tdh中文字幕5页、創(chuàng)傷弧菌的溶細(xì)胞毒素基因vvhA和擬態(tài)弧菌的熱不穩(wěn)定型溶血素基因vmhA的成熟蛋白基因片段進(jìn)行串聯(lián)，得到三聯(lián)融合毒素基因tdh-vvhA-vmhA亚洲男女精品视频网站。

Chapter 3, 4 and 5 report studies on several partially folded fragments, two denatured fragments and a folded fragment, respectively

第三免费人成网站在线观看不要钱、四、五章分別報告了對核酸酶的部分折疊片段91精彩国产，變性態(tài)和一個折疊的小片段的研究黄片在线www。摘要如下

He saw the moon regathering itself insidiously, saw the heart of the rose interwining vigorously and blindly, calling back the scattered fragments, winning home the fragments, in a pulse and in effort of return.

他看到月亮偷偷的重新聚集起來，看到它一如玫瑰的花心一般日韩免费一区二区三区免费视频道，在摸索中有力地交織著96在线播放，召喚著那些四散的碎片，脈動著努力說服它們回到家的懷抱五月婷丁香综合网。

Sequences were aligned by BLAST method. Results showed that 309bp fragments shared high similarity with orfl38 in Ogu CMS radish and Brassica cybrids of Ogu CMS radish, which 172 nucleotide sequences and 58 amino acids were same among them. And 689bp fragments shared high similarity (100%) with Pol orf224 in B.napus, which 677 nucleotide sequences and 225 amino acids were same among them.

同源性分析結(jié)果表明色欲黄色视频免费，利用orf138引物所獲得的309bP大白菜mtDNA特異片段均與蘿卜Ogu CMS、甘藍(lán)型油菜Ogu CMS蘿卜體細(xì)胞雜種所具有的Ogu orf138高度同源日韩一级无码AV毛片免费，二者有172個核苷酸完全相同韩日精品高肏操穴视频免费，有58個氨基酸完全相同；orf224引物所獲得的689bP大白菜mtDNA特異片段與甘藍(lán)型油菜的Pol orf224高度同源亚洲一区偷情视频，二者有677個核苷酸完全相同插插插插插爽，有225個氨基酸完全相同，同源性均達(dá)到100%黄色视频一级免费观看视频在线。