Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本論文的關(guān)鍵和難點，我們對文獻(xiàn)方法進(jìn)行改進(jìn)：文獻(xiàn)方法用氫氧化鈉與3，5-二甲基-4-羥基苯甲酸反應(yīng)制得3在线日韩素人自拍网站免费观看视频，5-二甲基-4-羥基苯甲酸鈉，再用3一级黄色网站毛片，5-二甲基-4-羥基苯甲酸鈉與芐氯作用制備3无码午夜视频，5-二甲基-4-羥基苯甲酸芐酯，我們用氫氧化鉀代替氫氧化鈉，使合成取得成功国产白嫩白丝护士在线播放；在合成2'一级黄色生活视频一区二区，6'-二甲基-4'-苯基-吖啶-9-甲酸酯時，文獻(xiàn)方法對粗產(chǎn)品進(jìn)行兩次硅膠柱層析純化日韩s8精品无码久久久久影院，第一次柱層析用氯仿／乙酸乙酯(4∶1黄色另类视频，V∶V)作溶劑和淋洗劑，第二次柱層析用己烷／丙酮(2∶1特级婬片欧美高清视频,，V∶V)作溶劑和淋洗劑非洲一二三区激情视频，按照文獻(xiàn)方法得到的不是所需要的化合物，我們只進(jìn)行第一次柱層析純化鸥美成视频，然后用己烷／丙酮(2∶1老黄视频欧美狂野，V∶V)進(jìn)行研磨变态视频的在线看网站，過濾www在线观看视频一级，洗滌，除去溶于己烷／丙酮(2∶1h爱视频，V∶V)的部分白白看看首页永久免费观看视频，得到所需要的產(chǎn)品。

The research results indicated that the first cross-linking between the HPAM and Cr3+ in ground condition was propitious to reduce leaking of blocking agent in oil layer, and the second crosslink action between acylamino of HPAM and organic phenolic aldehyde can form the high intensity gel and it can endure high temperature.

研究結(jié)果表明欧美午夜三级缴情性影院，HPAM羧基與Cr~(3+)離子在地面條件下的一次交聯(lián)作用有利于減少堵劑在油層的漏失量手机日本黄色电影网站，HPAM酰胺基與有機(jī)酚醛在地層條件下的二次交聯(lián)作用，能夠形成耐溫高強(qiáng)度凝膠女生精黄色网站。

Objective: To explore the feasibility of the combination of psuedo-ligand affinitive chromatography and gel chromatography in purifying RIP from bitter gourd and the cytotoxicity of the RIP.

結(jié)果： 運(yùn)用硫酸銨分級沉淀盗摄网址、Blue Sepharose CL-6B假配基親和層析、Sephadex-G75凝膠層析等方法提取a毛片网站全部播放、純化得到了高純度的苦瓜RIP羞羞网在线，并且能夠獲得較高的得率；一定作用劑量的苦瓜RIP不僅能夠抑制HL-60細(xì)胞的增殖活性黄色视频网址在线播放，還可以誘導(dǎo)其發(fā)生凋亡。

Using ammonium sulfate precipitation, Blue Sepharose CL-6B psuedo-ligand affinitive chromatography and Sephadex G-75 gel chromatography, we obtained the typeⅠRIP with high purity, the feasibility of this method has been approved by SDS-PAGE results.

結(jié)果顯示：1在线视频观看欧美。運(yùn)用硫酸銨分級沉淀、Blue Sepharose CL-6B假配基親和層析中国精品jizzjizz、Sephadex-G75凝膠層析等方法提取www.youjizz.com日本、純化得到了高純度的苦瓜RIP，并且本方法提取效率較高成人日韩欧美一区二区免费，所獲得的RIP在SDS-PAGE上呈現(xiàn)為單一條帶欧美性爱视频欧美。

The carboxyl groups of HPAM replace the H〓O ligands of AlCA, which results in the formation of HPAM-AlCA gel.

首次用實驗證明了：HPAM大分子中與AlCA反應(yīng)的基團(tuán)為羧基日韩三级视频免费，酰胺基不參與交聯(lián)反應(yīng)。

Dynamic viscoelasticity of curvielan,a novel gel-forming exopolysaccharide produced by soil bacterium Alcaligenes sp.Q9415 was studied.

對產(chǎn)堿桿菌Q9415胞外多糖的動態(tài)粘彈性進(jìn)行了研究久久免费高清视频。

39 Qinghai Alectoris graeca were used to study isozyme pattern of serum amylase and activity with vertical polypropylene gel electrophoresis and spectrophotometry, respectively.

方法］采用聚丙烯酰胺凝膠垂直平板電泳法對39只青海石雞血清淀粉酶同工酶酶譜進(jìn)行研究久久影院无码专区人妻，并利用比色法時其淀粉酶活性進(jìn)行測定。

The characters of the composition, which is double component sealant made from 1-3% alginic acid sodium and 20% CaCl2 solution, is best in the biological experiment. Before new medical glue appeared in clinical, this gel is hopeful to be applied in clinic and provide theory basis for clinical anastomosis.

質(zhì)量分?jǐn)?shù)為1%~3%的海藻酸鈉水溶液與質(zhì)量分?jǐn)?shù)為20%的CaCl2組成的雙組分密封膠欧美一级黄色淫秽肏屄视频，生物實驗中各項性能良好wwwav免费在线，在新的醫(yī)用膠面世以前黄色网页免费看，可以作為血管粘接吻合密封劑，有望在臨床中實驗推廣欧美视频免费在线，為臨床血管粘接吻合應(yīng)用提供理論依據(jù)。

Using normal alkalic shower gel will destroy the weak acidic environment and weaken the protective action.

但使用一般堿性沐浴露黄色工厂网久久综合，會破壞私處的弱酸環(huán)境午夜电影网理论片，減弱保護(hù)作用。

The porous TiO2 anatase nanometer thin films were prepared from alkoxide solutions containing polyethylene glycol by sol-gel method.

銳鈦礦型多孔TiO2納米薄膜可以從含聚乙二醇的鈦酸鹽溶膠前驅(qū)體中通過溶膠-凝膠法制備91久久高清国产自产拍。