Known differential expression gene: In the 46 points of known differential expression gene, there were down - regulate gene expression 41 points and up- regulate gene expression 5 points; twenty - eight genes related to hypoxia/reoxygenation had not been reported and 18 genes had been reported.

已知差異表達基因：在46個已知差異表達基因中色伊人欧美，表達下調的基因有41個，表達上調的基因有5個，未見報道的與缺氧/復氧處理相關基因23個18禁黄色网站免费，已報道的有18個。

The interaction between M. grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence gene and the host resistance gene.

稻瘟病菌與水稻的互作符合經(jīng)典的基因對基因學說日韩播放，二者的不親和互作是無毒基因與抗病基因相互作用的結果人人人人色人人人人人人。

The interaction between M.grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence gene and the host resistance gene.

由Magnaporthegrisea(無性世代為Pyricularia grisea或Pyculariaoryzae)引起的稻瘟病是世界農業(yè)生產(chǎn)上危害很嚴重的病害之一[1]。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

為了克隆微小牛蜱Bm86 基因及構建該基因的表達載體，以微小牛蜱饑餓幼蜱的破解物提取的總RNA為模板免费观看未18禁止网站国产，參照已發(fā)表的微小牛蜱Bm86基因的核苷酸序列操操日日日操，設計了1對引物一级黄色一级黄色一级黄色一级黄色a级毛片，采用RT-PCR技術獲得微小牛蜱Bm86基因黄片AAAAAAAAAAAAA；將Bm86基因克隆入載體，并進行序列分析国产一级A婬片试看26分钟，結果證明，克隆的Bm86基因序列與GenBank上登錄的Bm86基因序列的同源性達97.4%日韩亚洲一区二区三区，而且該序列包含完整的開放閱讀框国产69a片免费看。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

為了從分子水平闡明十字花科植物間的親緣進化關系，給植物種質資源的創(chuàng)建提供理論依據(jù)，試驗根據(jù)課題組已報道的CYP86MF基因編碼的氨基酸保守區(qū)域設計特異引物，運用PCR技術分別從十字花科6個屬11個物種中分離克隆到了CYP86MF基因的同源序列美女被操逼，經(jīng)比較分析国产无码一级视频，結果表明：這些同源序列的相似性達80%以上免费在线视频网站，所推導的氨基酸序列相似性達70%以上，且兩者種間差異分別為1.0%~ 5.7%和2.6%~ 7.3%，屬間差異分別是5.6%~ 22.5%和7.3%~ 31.2%久久久久亚洲AV无码专区电影；由氨基酸序列構建的分子系統(tǒng)樹可知，在親緣進化關系上蕓薹屬與蘿卜屬較近，其他依次為蔊菜屬婷婷色网址导航、擬南芥屬、薺菜屬久久精品无码一级毛片，而與諸葛菜屬最遠日本中文字幕在线视频二区。

Small interference RNA homolog to silenced gene in sequenceis indued into cell to clearage the mRNA of target gene. Many studies havedemonstrated that RNAi is an effective tool for gene diagnosis and gene therapy.

我們根據(jù)文獻報道選擇EZH2基因cDNA的461～481堿基為靶序列，構建EZH2-siRNA表達質粒欧美成年性开放视频在线播放，并研究其對人膀胱癌EJ細胞EZH2基因表達及生長抑制作用久久精品青春五月天综合网。

Objective: To colone SOD gene, construct combinant of retrovirus SOD gene, make a basis of gene transfection and over-expression of gene therapy.

目的 通過克隆SOD基因，構建逆轉錄病毒-SOD真核表達載體轉染復合體作爱综合，為基因轉染及SOD的過表達提供基礎国内免费合计自拍偷拍小视频，為將來的基因治療進行準備，也為深入研究SOD功能及SOD的開發(fā)創(chuàng)造條件日本中文在线中文幕。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鯉科魚類Beta-肌動蛋白基因序列国产精品黄色视频，采用長片段PCR技術亚洲无码啪啪啪视频，克隆了青魚β-actin基因DNA片段，并構建了青魚β-actin基因5'啟動調控區(qū)綠色熒光蛋白表達載體，通過顯微注射泥鰍胚胎和COS-7細胞轉染實驗亚洲欧洲成人在线观看，證明了所克隆的青魚β-actin基因5'啟動調控區(qū)具有啟動調控功能，并用之來構建&全魚&基因的啟動子部分久久国产精品电影网。

Five plasmids for the expression of doxA gene, which were designated as pYG57, pYG502, pYG503, pYG505 and pYG506 were constructed so that the cloned doxA gene were under the control of either promoter of melanin gene or erythromycin resistant gene or their tandem promoters, and a fd terminator downstream.

構建了五個doxA基因鏈霉菌表達質粒pYG57、pYG502、pYG503久久av一区、pYG505和pYG506久久免费色色视频，使得doxA基因在上游的Pmel啟動子、紅霉素抗性基因啟動子或者兩者的串聯(lián)啟動子99偷拍精品视频，以及下游的fd終止子控制之下男女做爽爽爽视频免费网站。

We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.

根據(jù)國內外最新文獻報道，篩選出對β-內酰胺類抗生素耐藥的mecA基因及輔助因子femA亚洲国产精品色一区二区三区在线；對大環(huán)內酯類及林可霉素類抗生素耐藥的ermA、ermC、msrA基因日韩免费午夜短片在线，對喹諾酮類抗菌藥物耐藥的norAA片亚洲、欧美视频、grlA和gyrA基因及對四環(huán)素類抗生素耐藥的tetM基因作為擬選的主要耐藥基因，分別設計PCR擴增的特異性引物色酷色视频，進行克隆。