In this study, what had been used inculding CBF1 and CBF3 of CBF family, its downstream COR15a gene , regulating element CRT/DRE in promoter of COR15a gene, Cloning CBF1 and CBF3 from Arabidopsis thaliana, and Cloning ApCOR15a gene from Arabis pumila of xinjiang. On the basis of plasmid pBI121,being used CaMV35S promoter and SAR iductivity promoter synthesized by PCR, six plant expression vectors had been constructed: pCB111, pCB111-1, pCB112,pCB112-1,pCB113, pCB113-1, and then transformed to Agrobacterium GV3101 by electics.

本研究選用了CaMV35S組成型啟動(dòng)子和人工合成的由水楊酸誘導(dǎo)的啟動(dòng)子SAR美女黄网站国产免费久99、擬南芥CBFs家族中的CBF1和CBF3先锋每日资源涩涩、新疆小擬南芥COR15a基因及其CRT/DRE順式作用元件構(gòu)建了不同組合的六個(gè)植物表達(dá)載體：pCB111免费超碰在线免费在线观看、pCB111-1人人操日日插、pCB112免费黄片一级、pCB112-1、pCB113和pCB113-1欧美一级粗暴又裸又黄，并采用農(nóng)桿菌介導(dǎo)的葉盤(pán)轉(zhuǎn)化法轉(zhuǎn)化煙草黄色国产精品自拍视频网站，獲得了大量的抗性植株。

The cowpea trypsin inhibitor gene and the bar gene discovered in recent years are found to be used for insect-resistant and anti -herbicide respectively because of their broad spectrum and are used in gene improvement of many crops widely now. Using the peanut Arachis hypogaea L. cv. Shanyou 523 and Arachis hypogaea L.

在多種抗植物蟲(chóng)害青草青草久久伊人热、耐除草劑基因中jizz国产视频，豇豆蛋白酶抑制劑基因(cowpea trypsin inhibitor，CpTI)和耐除草劑基因因其抗蟲(chóng)和耐除草劑的廣譜性而成為效果最為理想的外源基因久久精品国产精品久久96，被廣泛地應(yīng)用于農(nóng)作物抗蟲(chóng)aA电影黄色电影网、耐除草劑基因的改良上。

Upon the optimized Agrobacterum-mediated transformation systems mentioned above, the herbicide-resistant gene bar has proved to be successfully incorporated into genomes of the Cynodon dactylon after PCR assay of hpt gene and bar gene, GUS histochemical assay and hpt resistance evaluation.

經(jīng)潮霉素抗性基因hpt和除草劑抗性基因bar的PCR分析亚洲中文字幕乱码在线精品、GUS組織化學(xué)染色和潮霉素抗性鑒定日韩黄页无码精品视频，證實(shí)除草劑抗性基因bar已成功整合到狗牙根基因組。

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：為建立一種利用多重pcr技術(shù)檢測(cè)和鑒定沙門(mén)氏菌韩国日本在线精品网站、單核細(xì)胞增生李斯特菌人人人人人艹、金黃色葡萄球菌和蠟樣芽孢桿菌的方法，根據(jù)沙門(mén)氏菌侵襲力蛋白a基因啊啊啊啊www、單核細(xì)胞增生李斯特菌蛋白轉(zhuǎn)錄調(diào)控基因国产三级激情在线视频、金黃色葡萄球菌自溶素基因、蠟樣芽孢桿菌的促旋酶b亞單位基因設(shè)計(jì)引物久草巨乳，進(jìn)行多重pcr擴(kuò)增久久精品中文字幕丝袜，并對(duì)多重pcr反應(yīng)體系進(jìn)行優(yōu)化。

Domestic Violence in Pregnancy; Psychosocial Characteristics; Postpartum Depression; Risk Factors; Neonate; Glutamate; r-aminobutyric acid; plasma cortisol; COMT gene; 5-HT gene; Single nucleotide polymorphism; Temperament; Mental Development Index; Physical Development Index; Gene-Environment Interaction; Neural Network; Biomathematic Model

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The results showed that the organization and gene order of the mitochondrial genomes of these bivalve species studied were different from each other. The size, organization, gene numbers, and gene order of mitochondrial genomes in bivalves at different taxa were different.

結(jié)果發(fā)現(xiàn)，雙殼貝類(lèi)線(xiàn)粒體的基因組結(jié)構(gòu)日韩中文字幕在线大全视频、基因排列順序均互不相同考屄视频；不同目、科和屬之間線(xiàn)粒體基因組的大小免费播看完整大片40分钟、基因排列方式以及基因種類(lèi)也存在明顯的差異外国在线播放黄色视频，尤其是基因排列方式?jīng)]有明顯的規(guī)律。

For the futher research of the change in gene level,we used microarrary analysis to study gene expression in the olfactory blub in the model of AD,for the aim to analyse the relationship between olfactory and AD in the level of gene.

在得到的811個(gè)差異表達(dá)基因中進(jìn)行第一步初篩啪啪流水视频，挑選出23個(gè)表達(dá)差異明顯的基因亚洲黄色免费在线，這些基因包括與神經(jīng)退行性疾病發(fā)病機(jī)制、細(xì)胞凋亡途徑国产黄色网页、鈣離子信號(hào)通路等相關(guān)的基因欧美色色日韩。

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本實(shí)驗(yàn)從抗除草劑轉(zhuǎn)基因Bobwhite小麥中，利用PCR克隆的方法擴(kuò)增出bar基因全長(zhǎng)欧美性爱影视在线观看，并在原核表達(dá)系統(tǒng)中表達(dá)福利网在线观看亚洲AV，鑒定表達(dá)蛋白的活性，將能夠正確編碼PPT乙酰轉(zhuǎn)移酶的bar基因片段三级片免费一区，經(jīng)過(guò)適當(dāng)?shù)男揎棙?gòu)建入真核表達(dá)載體日本强伦姧人妻免费无。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通過(guò)GenBank中的中華絨鰲蟹線(xiàn)粒體16S rDNA序列設(shè)計(jì)引物，利用PCR擴(kuò)增方法韩国三级2022最新中字幕在线观看，以beta-actin做內(nèi)參18岁以下禁止浏览的黄色网站，檢測(cè)了中華絨螯蟹肌肉、鰓99热在线精品播放、精巢和精子中線(xiàn)粒體16S rDNA擴(kuò)增情況欧美性爱视频亚州，發(fā)現(xiàn)各組織或細(xì)胞beta-actin擴(kuò)增片段大小、條帶寬度和亮度基本一致学生妹网址，表明各模板DNA量基本一致www精品；而在同一DNA濃度下超碰插，16S rDNA的擴(kuò)增片段長(zhǎng)度雖一致，但其產(chǎn)物量存在明顯差異男女免费视频一区二区三区，精子16s rDNA產(chǎn)物量顯著低于其他3種組織羞羞无遮掩免费视频在线观看，其條帶寬度和亮度很弱；16S rDNA擴(kuò)增產(chǎn)物經(jīng)測(cè)序分析及比對(duì)證明與已有序列完全吻合亚洲女人色色。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通過(guò)RFLP及微衛(wèi)星多態(tài)性分析久草A片在线视频，應(yīng)用兩位點(diǎn)及多位點(diǎn)連鎖軟件，建立了中國(guó)人WD基因在D13q14.2-3區(qū)域的精細(xì)遺傳連鎖圖譜sese亚洲图片，從而首次對(duì)中國(guó)人WD基因進(jìn)行了精確定位第一福利视频精品；②WD基因突變研究：應(yīng)用PCR-SSCP及DNA測(cè)序技術(shù)，對(duì)39個(gè)家系45名WD患者進(jìn)行該致病基因的21個(gè)外顯子突變篩選国产av无码影音先锋，發(fā)現(xiàn)WD基因5號(hào)外顯子存在新的T插入突變强奸熟女的视频，并證實(shí)中國(guó)人WD基因的突變熱點(diǎn)為8號(hào)外顯子，突變形式為Arg778Leu色色五月天A，其頻率為22.8%国产性爱免费在线；③WD的癥狀前診斷和雜合子檢出：應(yīng)用DNA重組技術(shù)對(duì)79個(gè)家系進(jìn)行基因診斷，成功地進(jìn)行了WD的癥狀前診斷和雜合子檢出www.在线黄片.com，并建立了WD的基因篩選的PCR-Msp 1酶切方法一级片免费播放。