Therefore, the technique of gene SOEing is a simple and effective method for gene recombination and mutation in vitro, which is significant to study structurally and functional characteristic of gene in high plants.

因此1024亚洲免费视频，重疊PCR技術(shù)是一種簡單有效的進(jìn)行體外基因重組和突變的方法，對于研究高等植物功能基因的結(jié)構(gòu)功能特性具有重要的意義国产欧美很黄。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

對云南省10個(gè)地區(qū)的30余個(gè)縣市地區(qū)進(jìn)行了植原體病害的調(diào)查采集工作国产黄一级视频网站；采用植原體16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原體核糖體蛋白基因引物（rpF1/R1）對所采集到的病害標(biāo)樣總DNA進(jìn)行PCR擴(kuò)增，根據(jù)植原體特異擴(kuò)增條帶的出現(xiàn)免费在线观看欧美，共鑒定出泡桐叢枝国产一级免费无病毒黄片、苦楝叢枝、仙人掌叢枝一级欧洲性爱视频、蟹爪蘭叢枝来个毛片网站、柑桔小葉、矮牽牛扁莖等12種植原體病害日本三级片久久，初步明確了云南省植原體病害的種類6080yy午夜三级中文在线；通過RFLP技術(shù)、克隆及測序技術(shù)獲得其16SrRNA及部分核糖體蛋白基因的近全長序列（已在GenBank中共登錄近20余個(gè)相關(guān)序列）男人深夜天堂网站男人玩游戏女子想要，通過序列比較分析欧美日韩国产一区，明確了上述植原體株系中這兩個(gè)基因的特征并確定了云南省植原體株系的種類、分類地位及遺傳相關(guān)性精品亚洲黄色视频在线观看；對一些發(fā)生在草本植物上的植原體進(jìn)行了株系保存免费观看国产裸体美女网站；在國內(nèi)核心期刊及一般期刊發(fā)表研究論文 9 篇，申請技術(shù)發(fā)明專利1項(xiàng)p怕怕免费网站；初步建立了有關(guān)植原體病害的網(wǎng)頁99久久精品无码专区；培養(yǎng)研究生4名。

After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene. Moreover, the whole sequences of the two Wx genes of nonglutinous rice reported by others were also aligned, and another two pairs of dominant STS molecular markers were designed to discriminate Wxa from Wxb based on the special site between the two genotypes. Then, corresponding rice genotypes were identified by the established new markers.

通過對前人公布的糯性水稻蠟質(zhì)基因全序列測定結(jié)果的分析91干屄，根據(jù)糯性水稻與非糯性水稻蠟質(zhì)基因序列位點(diǎn)的差異亚州黄色一级无码，設(shè)計(jì)了兩個(gè)基于PCR擴(kuò)增反應(yīng)的可特異識別糯性水稻的顯性和共顯性STS分子標(biāo)記三级片精品视频；同時(shí)在前人公布的非糯性水稻蠟質(zhì)基因的不同類型的全序列比對分析的基礎(chǔ)上，選取了兩種基因型特定的差異位點(diǎn)多人乱P过瘾视频免费，設(shè)計(jì)了兩個(gè)基于PCR擴(kuò)增反應(yīng)用于特異識別這兩種Wx基因的顯性STS分子標(biāo)記chaopeng97，并用新建立的標(biāo)記對相應(yīng)的水稻基因型進(jìn)行了檢測。

The bioinformatics analysis showed that the gene had the highest homology with the gene of Penicillium janthinellum, which reached up to 76%. The first 26 amino acids might be a signal peptide sequence with the hydrophobicity of up to 2.63. The CBHⅠ gene consisted of the catalytic domain, convergence zone and cellulose-binding domain and its tertiary structure was mainly composed of 3-sheets.

分別擴(kuò)增到鵝源草酸青霉F67纖維素酶CBHⅠ基因片段A(EU574736)青青久操手机在线中文字幕、5'端序列B1(EU603295)国产一级A片AV在线、3'端序列B2(EU652768)及全長基因C(EU727171)，并成功構(gòu)建真核表達(dá)載體pPIC9K-CBHⅠ国产免费性交a片；生物信息學(xué)分析表明一a级黄片免费看，該基因蛋白序列與微紫青霉氨基酸序列同源性最高，達(dá)76%最新欧美操屄视频，前26個(gè)氨基酸為信號肽序列50熟妇，疏水性可達(dá)2.63，由催化功能域毛片黄色网站在线观看、銜接區(qū)和真菌性纖維素結(jié)合域構(gòu)成男人的天堂视频，其三級結(jié)構(gòu)主要為β-sheet。

From the calculation of two different similarity measures—Pearson correlation coefficient and Spearman correlation coefficient, we found that about 94 percent of gene expression regulatory pathways are correlatively expressed in more than 4(including 4) sets of expression profile data, and it presents additional evidence for the correlation between gene function and its expression in the view of gene expression regulatory pathways.

通過Pearson相關(guān)系數(shù)和Spearman相關(guān)系數(shù)兩種相似性測度的分析永久女性毛片免费看，我們發(fā)現(xiàn)有94%（15條）的基因表達(dá)調(diào)控通路內(nèi)的基因在大于等于4套的表達(dá)譜數(shù)據(jù)中是共表達(dá)的黄色网站黄色网址视频，以上結(jié)果從基因表達(dá)調(diào)控通路的角度，證實(shí)了基因功能與基因表達(dá)之間存在著一定的相關(guān)性2019人妻巨乳超碰。

The prolactin gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Single nucleotide polymorphisms of exon 1 to exon 5 of PRL gene were detected in high fecundity breeds and low fecundity breeds (Dorset, Texel and Corriedale sheep) with five pairs of primers by PCR-SSCP.

設(shè)計(jì)5對引物欧美天欧美天堂aⅴ在线，采用PCR-SSCP技術(shù)檢測催乳素（prolactin，PRL）基因外顯子1至外顯子5在高繁殖力綿羊品種和低繁殖力綿羊品種（多賽特理论午夜、特克塞爾和考力代）中的單核苷酸多態(tài)性美国一级强奸片，同時(shí)研究該基因?qū)π∥埠蚋叻敝沉Φ挠绊憽?/p>

The gonadotropin releasing hormone receptor gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 1, exon 2 and exon 3 of GnRHR gene in both high fecundity breeds and low fecundity breeds (South African Mutton Merino, Corriedale and Chinese Merino sheep) by PCR-SSCP. Only the products amplified by primers P4 and P7 displayed polymorphisms.

設(shè)計(jì)8對引物，應(yīng)用聚合酶鏈?zhǔn)椒磻?yīng)-單鏈構(gòu)象多態(tài)性技術(shù)檢測促性腺激素釋放激素受體（gonadotropin releasing hormone receptor国产操妓女的屄视频， GnRHR）基因外顯子1中文字幕永久在线、外顯子2和外顯子3在高繁殖力品種和低繁殖力品種（南非肉用美利奴、考力代和中國美利奴綿羊）中的單核苷酸多態(tài)性人人澡人人摸人人干，同時(shí)研究該基因?qū)π∥埠蚋叻敝沉Φ挠绊憽?/p>

Over the last 10 years, antisense transgenic plants have been used as tools to address this and have revealed some unexpected findings. In this article, chloroplast tpi gene from spinach and tandem genes of chloroplast tpi gene from spinach and cytoplast fba gene from rice were transferred into filamentous, heterocystour cyanobacterium Anabaena sp.

將菠菜葉綠體tpi和水稻胞質(zhì)fba的串聯(lián)基因連接在光誘導(dǎo)的高效啟動子PpsbA下游免费观看国产黄页网站，克隆入pDC-08中構(gòu)建了既能在大腸桿菌又能在藍(lán)藻中表達(dá)的穿梭表達(dá)載體pDC-AT，并通過三親接合轉(zhuǎn)移轉(zhuǎn)入魚腥藻7120a级线手机免费观看。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

結(jié)果：聚合酶鏈反應(yīng)擴(kuò)增結(jié)果與目的片段位置一致一级A片全网免费观看，測序結(jié)果表明為肝再生增強(qiáng)因子mRNA，基因全長378 bp欧美性爱在线综合，在人偷拍一级黄片操操操视频、牛、小鼠久久一级黄片羞羞、斑馬魚等物種中高度保守国产视频福利网站。

In order to understand insertion/delation polymorphism of the angiotensin-converting enzyme gene in pilots,and to explore the relationship between ACE gene I/D polymorphism and the perfomance of the pilots,the polymerase chain reaction was used to determine the genotypes for an I/D polymorphism in intron 16 of the ACE gene in 118 pilots and 96 healthy subjects as controls.

為了解飛行員血管緊張素轉(zhuǎn)換酶基因插入或缺失多態(tài)性情況在线视频自拍网，探討ACE基因多態(tài)性與飛行員耐力可能的關(guān)系，用聚合酶鏈反應(yīng)擴(kuò)增技術(shù)檢測118例飛行員和96例健康對照者的ACE基因I/D多態(tài)性一级黄片在线免费看。