The distribution of hybrid chlorosis Ch1 gene and the T-type cytoplasm fertility restoring genes in Chinese endemic wheats were studied using the testors QA1104 (T-type cytoplasm sterile line, with hybrid chlorosis Ch2 gene) and Khapli emmer (with hybrid chlorosis Ch1 gene).

本研究以T型不育系QA1104（具有雜種黃化基因Ch2）和Khapli（具有雜種黃化基因Ch1）為測驗種欧美日韩综合久久精品A片app，對中國特有小麥等六倍體小麥類型和一些四倍體小麥類型中的T型胞質(zhì)育性恢復(fù)基因和雜種黃化Ch1基因的分布進(jìn)行了研究久爱www人成视频在线观看免费。

Silencing gene is a kind of transferred gene or invaded virus or endogenesis gene.

發(fā)生沉默的基因可以是外源性轉(zhuǎn)移基因里番纯肉H网站在线，也可以是入侵的病毒或宿主內(nèi)源性基因人人澡人人摸人人干。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根據(jù)Bt殺蟲蛋白Cry1A和豇豆胰蛋白酶抑制劑的高級結(jié)構(gòu)、殺蟲機(jī)理及昆蟲體內(nèi)腸激酶的作用機(jī)理黄黄的在线网站，本著盡量不影響二者功能的原則av资源观看，設(shè)計出了構(gòu)建Cry1A和CpTI融合殺蟲基因CryCI的方案，即將CpTI基因的編碼序列連接到GFM Cry1A基因編碼區(qū)3'端18岁免费看黄片，并在其間用一段編碼腸激酶切割位點的序列相連A片勉费视频网站。

With the competent germinating epicotyl of cotton as receptor in exogenous gene transformation, NPTII (neomycin phosphotransferase Ⅱ) gene carrying selection marker and GFP reporter gene were mediated into cotton by agrobacterium-mediated transformation. The pre-culture time, bacterial-immersing time, co-culture time and antibiotics concn., etc of using agrobacterium-mediated transformation in the transformation of competent germinating epicotyl of cotton were studied.

方法］以棉花感受態(tài)萌動的上胚軸作為外源基因轉(zhuǎn)化的受體婷婷五月天激情综合网，用農(nóng)桿菌介導(dǎo)法將攜帶有篩選標(biāo)記NPTII基因及GFP報告基因?qū)朊藁ǎ芯苛宿r(nóng)桿菌介導(dǎo)法用于棉花感受態(tài)萌動上胚軸轉(zhuǎn)化的預(yù)培養(yǎng)時間精品一本无码久久，浸菌時間，共培養(yǎng)時間及抗生素處理濃度等无码少妇一区二区三区av。

Repens L. and E. intermedia Nevski by using SSR, in order to provide science theories basis and base to further study E. repens L. drought resistance gene molecular marker, discover excellent drought resistance gene source , fast and efficiently select with the molecular marker of drought resistance gene catena for E.

通過對20 份來源不同的偃麥草與中間偃麥草種質(zhì)材料在室內(nèi)模擬逆境和田間干旱條件下進(jìn)行抗旱材料的篩選和鑒定中国AAA片爽快免费，應(yīng)用SSR 技術(shù)對偃麥草抗旱分子標(biāo)記進(jìn)行引物篩選，為進(jìn)一步深入研究偃麥草與中間偃麥草抗旱基因分子標(biāo)記日本不卡的涩涩网站、發(fā)掘優(yōu)異抗旱基因源欧美韩一区，快速高效篩選出與偃麥草與中間偃麥草抗旱基因連鎖的分子標(biāo)記提供科學(xué)理論依據(jù)和基礎(chǔ)。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基礎(chǔ)上進(jìn)一步改造国产精品路边搭讪素人，構(gòu)建截去AIF基因線粒體定位信號人人色人人射人人操，F(xiàn)AD結(jié)合結(jié)構(gòu)域和NADH結(jié)合結(jié)構(gòu)域（1-400位氨基酸）編碼序列的AIF△1-400基因，將其克隆入pcDNA3和pIRES2EGFP真核表達(dá)載體久久只精品99品免费久苍井空，用脂質(zhì)體法轉(zhuǎn)染HeLa細(xì)胞熟妇天天干视频，通過熒光顯微鏡觀察，MTT檢測等方法檢測該基因在轉(zhuǎn)染細(xì)胞中的表達(dá)及其對腫瘤細(xì)胞的促凋亡活性91黄色大片在线观。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

結(jié)果用 PCR從酵母基因組DNA中擴(kuò)增出大小約1.5 kb的suc2基因片段人人操,人人舔人人干，序列測定表明除585位有一點突變外，所得suc2基因與文獻(xiàn)報道相同裸体女人的视频一级A片，構(gòu)建的定位突變載體導(dǎo)入酵母細(xì)胞后免费狼友，得到4株營養(yǎng)型符合的突變體，PCR表明這些突變體中均有suc2基因的同源重組一级黄网，取其中1株的PCR產(chǎn)物進(jìn)行序列測定證實了同源重組的發(fā)生99riAV日日久。

It can cause heavy losses of tomato production and friuts' quality. According to the researches of internal and external country, This review summarized the main symptoms of Fulvia fulva of tomato caused by cladosporium fulvum , the biological properties, including to form character, temperature and humidity of mycelium and spore, the outbreak of the disease, regularity of epidemic, race differentiation of different area, Maofen 802,Shuangkan 2No.should be chosen. In resisting disease ,mode of action, gene clone,gene structure,gene function and control methods of the disease.

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Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫鏈絲菌素抗性基因的pIJ903的單—BamHI位點天天操天天色天天射，同向插入了FR-008生物合成基因簇兩個最遠(yuǎn)端的4.0kb和1.5kb片段，并在二者之間插入了可在鏈霉菌FR-008和變鉛青鏈霉菌中表達(dá)的阿泊拉霉素抗性基因国产一区色，同時也插入了有助于將質(zhì)粒引入鏈霉菌的oriT片段欧美一区1区三区3区公司，從而構(gòu)建出了置換整個基因簇的基因置換質(zhì)粒pHZ691。

The DNA fingerprints of 4 biotypes of GM population from 7 locations were analyses by AFLP.Base on the fine mapping of resistance gene Gm6 against all 4 Chinese GM biotypes by RAPD and SSR methods respectively,the STS markers and SSR markers linked to gene Gm6 were used for breeding through marker assisted selection.Some new resistant germplasm with Gm6 gene were created.And 6 cultivars and 6 lines of two-line hybrid rice and 1 line of three-line hybrid rice against GM were bred and extended to the farmer.The technique system of MAS for resistant varieties against Asian rice gall midge was set up at Guangdong province of China.

用AFLP方法對從中國廣東省7個地點采集的4個生物型的DAN指紋進(jìn)行分析；在對用RAPD和SSR技術(shù)分別對抗中國4個稻癭蚊生物型的基因Gm6精細(xì)定位基礎(chǔ)上新赏网在线一区二区，用與Gm6緊密連鎖的STS和SSR標(biāo)記開展分子標(biāo)記輔助育種亚洲骚B视频在线，創(chuàng)造了一批抗稻癭蚊的新種質(zhì)，包括育成了6個栽培稻和6個二系雜交稻和1個三系雜交稻并在農(nóng)戶試種免费A片大大片，在中國廣東成功地建立了分子標(biāo)記輔助選育抗稻癭蚊品種的技術(shù)體系jizzwww。