It includes hardware equipments and foundational theory of the image digitization. This paper expatiates on some digital image processing technologies that the inspecting system may apply to the research and analysis of the collected glass-crack image. Using neighborhood average method and median filter retrains the image noises and make the image edge smooth. Then protract the histogram of the gray image, get the threshold of this image, utilize the threshold method and gray-level slice method to get a binary image, and detect the image edge. Finally calculate the area and perimeter of the glass-crack image, and use the circularity to judge whether this image is a glass-crack image.

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Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

結(jié)果： ①松果體摘除后大鼠下丘腦室旁核CRH 原位雜交和免疫組化的結(jié)果與假手術(shù)對照組相比均顯示陽性細(xì)胞數(shù)增多黑人AAAAAAAA片免费、陽性面積增加、平均灰度降低日韩人妻巨乳自慰，提示松果體摘除后下丘腦CRH 神經(jīng)元的表達(dá)增強(qiáng)18禁午夜老色批福利院；②松果體摘除大鼠補(bǔ)充褪黑素后国产一级淫片A片AA片男，下丘腦CRH 原位雜交和免疫組化染色增強(qiáng)呈劑量依賴性恢復(fù)，不過不論是低劑量組還是高劑量組国产在线精选，也不論是術(shù)后4 周還是術(shù)后8周，均不能恢復(fù)至正常日韩一级欧美一级二级在线观看视频；③松果體摘除后神經(jīng)垂體正中隆起CRH 免疫組化結(jié)果顯示陽性面積增加欧美一A片网站、平均灰度降低，提示下丘腦CRH 神經(jīng)元釋放CRH 增加轮奸网站；④ELISA 法檢測血清CRH結(jié)果示各組血清CRH 濃度差異沒有顯著性国产精品免费观看26uuu，表明松果體摘除及補(bǔ)充褪黑素對血清CRH 水平?jīng)]有顯著影響。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

結(jié)果： ①松果體摘除后大鼠下丘腦室旁核CRH 原位雜交和免疫組化的結(jié)果與假手術(shù)對照組相比均顯示陽性細(xì)胞數(shù)增多久草久草久草、陽性面積增加亚洲啪啪黄片、平均灰度降低，提示松果體摘除后下丘腦CRH 神經(jīng)元的表達(dá)增強(qiáng)日韩伊人自拍视频网站在线；②松果體摘除大鼠補(bǔ)充褪黑素后国产浓毛熟女免费视频，下丘腦CRH 原位雜交和免疫組化染色增強(qiáng)呈劑量依賴性恢復(fù)，不過不論是低劑量組還是高劑量組2019av亚洲天堂，也不論是術(shù)后4 周還是術(shù)后8周A片三集片在线hu免费，均不能恢復(fù)至正常；③松果體摘除后神經(jīng)垂體正中隆起CRH 免疫組化結(jié)果顯示陽性面積增加av网站直接看、平均灰度降低毛片视频免费看，提示下丘腦CRH 神經(jīng)元釋放CRH 增加；④ELISA 法檢測血清CRH結(jié)果示各組血清CRH 濃度差異沒有顯著性日韩性色自拍视频在线观看，表明松果體摘除及補(bǔ)充褪黑素對血清CRH 水平?jīng)]有顯著影響国产全黄A级A片一免费。

The characters of different illumination conditions are studied and the corresponding algorithms of gray image edge detection are proposed. In normal condition, the Sobel operator can be used to get the image edge. When the illumination is faint, the image is first enhanced by the MSR, and then the variance threshold algorithm is employed. While the light is strong, the image edge can be got by gray expand. At last, the instance image is detected.

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Finally, according to the characters that the inner and outer edge of double-layer eyelid are approximate parallel and the gray distribution changes sharply on the edge of inner eyelid, take the fitting curve acquired in first eyelid location as a template, and shift it down parallel to detect the position where gray value changes abruptly.

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The group disposed by Acetylspiramycin doesn"t show parallel results; The strip gray scale of exotoxin A decreased gradually with the increasing dosages of Azithromycin and Roxithromycin, which doesn"t show in group disposed by Acetylspiramycin and group PAO-JP2; The strip gray scale of lasR, detected by RT-PCR, decreased gradually with the increasing dosages of Azithromycin, which doesnt show in group PAO-JP2.Conclusions: There were obvious differences between PAO1 and PAO-JP2 in biology characteristics in vitro.

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