RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

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The Panasonic WV-NP1004 is a high quality, high efficiency, and high reliability color network camera.

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A high-performance modular exponentiation coprocessor, SEA, is presented here, and three novel ways are employed. First, a parallel binary modular exponentiation algorithm is used to decrease cycles, and a high radix Montgomery modular multiplication algorithm is modified to the radix based high radix Montgomery modular multiplication algorithm to increase the frequency; second when mapping algorithms to a systolic array, modular square and modular multiplication are alternatively computed to cover up the dependencies between iterations in the RBHRMMM algorithm and the bypass is used to eliminate the dependencies in the PBME algorithm; third, multipliers are split first, and then accumulations are compressed as partial products to decrease carry propagation delay in the critical path.

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But after ultra-high pressure treatment, the all free amino acid can be measured except alanine and cystine and the content of asparagines is lower. The content of free amino acid is the highest in ultra-high pressure sterilize milk, the pasteurism milk is second, the ultra high temperature sterilized milk is lowest.

但是牛乳經(jīng)過(guò)超高壓殺菌處理以后，除丙氨酸和胱氨酸未檢出外国性交狂操视频，且超高壓殺菌處理以后的天門(mén)冬氨酸的含量略低以外欧美激情高潮老外a一级，其余游離氨基酸均可檢出，而超高壓殺菌牛乳中游離氨基酸的含量最高黄色片黄色网站黄色片，巴氏殺菌乳中的含量次之色天堂综合，超高溫瞬時(shí)殺菌乳中的含量最低。

The soft soil has characteristics of high water content and low anti-shears strength and high compression coefficient and low penetrability and high sensitivity.

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The multiyear pentad mean data are used to analyze the mid_summer 100 hPa level circulation patterns and it is found that there are two balancing modes of the South Asian High: the Tibetan High and the Iranian High.

利用多年逐候平均資料，分析得到盛夏南亞高壓的兩類(lèi)平衡態(tài)-青藏高壓和伊朗高壓女高中生第一次破处，它們又各可分為東西部型第一页国产。

High performance concrete is high quality and high perdurable concrete .

高效混凝土是具有高質(zhì)量和高耐久性的混凝土。

All facilities of machinery have been imported from Korea, at the same time that they are blessed with high precision of production capacity, according to perfective technological process, high precision of test method, scientific manage system and so on that strickly make all produce process had high quality.

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The qualities of the ANN, high- nonlinear ,high- permissibility of error and high- robustness, self- adaptability, online work, and so on, are adequately used in the research.

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