Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

結(jié)果表明：利用電融合方法制備的小鼠四倍體胚胎在體外培養(yǎng)體系中經(jīng)歷細(xì)胞質(zhì)融合激情文学小说区另类小说同性、細(xì)胞核融合及細(xì)胞繼續(xù)分裂發(fā)育直到囊胚期的過程国产三级香港三级人妇，在細(xì)胞質(zhì)融合的時候胚胎卵裂球同體內(nèi)體外培養(yǎng)二倍體胚胎一樣一级片一级片一级片网址，呈現(xiàn)高度甲基化狀態(tài)538视频在线观看；在細(xì)胞核開始融合的時候欧美性色生活片在线观看视频，甲基化水平急速下降91精品国产自产在线观看直，在細(xì)胞核完全融合的時候甲基化水平達到最低點；隨著胚胎繼續(xù)分裂免费国产黄网站在线观看视频，胚胎甲基化水平逐漸增加老熟妇操B视频，在桑葚胚期甲基化水平最高；但是囊胚期四倍體胚胎內(nèi)細(xì)胞團同滋養(yǎng)層細(xì)胞甲基化熒光信號沒有差別最新日韩精品第一页，這與體內(nèi)體外培養(yǎng)二倍體囊胚內(nèi)細(xì)胞團細(xì)胞甲基化熒光強度高于滋養(yǎng)層細(xì)胞甲基化熒光強度不同免费精品国产自产拍在线观看。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

結(jié)果表明：利用電融合方法制備的小鼠四倍體胚胎在體外培養(yǎng)體系中經(jīng)歷細(xì)胞質(zhì)融合、細(xì)胞核融合及細(xì)胞繼續(xù)分裂發(fā)育直到囊胚期的過程中文自拍日韩视频在线观看，在細(xì)胞質(zhì)融合的時候胚胎卵裂球同體內(nèi)體外培養(yǎng)二倍體胚胎一樣免费看无码视频进入口，呈現(xiàn)高度甲基化狀態(tài)；在細(xì)胞核開始融合的時候丁香花在线观看视频在线，甲基化水平急速下降午夜性色一区二区免费视频，在細(xì)胞核完全融合的時候甲基化水平達到最低點；隨著胚胎繼續(xù)分裂99看黄，胚胎甲基化水平逐漸增加性爱视频人妻，在桑葚胚期甲基化水平最高；但是囊胚期四倍體胚胎內(nèi)細(xì)胞團同滋養(yǎng)層細(xì)胞甲基化熒光信號沒有差別红牛黄色视频，這與體內(nèi)體外培養(yǎng)二倍體囊胚內(nèi)細(xì)胞團細(xì)胞甲基化熒光強度高于滋養(yǎng)層細(xì)胞甲基化熒光強度不同性感美女毛片视频。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

結(jié)果：酪絲亮肽能顯著延長腹水型肝癌H_(22)小鼠的生存時間，給藥劑量為80μg／kg／d時療效最顯著男女干事网站，達到69.24％日日摸夜夜摸人人看亚洲综合，在20μg／kg／d-80μg／kg／d劑量范圍內(nèi)生命延長率為50-70％，給藥劑量與荷瘤鼠生存時間呈現(xiàn)一定量效關(guān)系www.亚洲综合；酪絲亮肽能顯著抑制人肝癌BEL-7402移植瘤裸鼠的腫瘤生長大线香蕉在线视频网站观看，給藥劑量為160μg／kg／d時療效最顯著，抑制率為44.03％欧美一级婬片A片护士，并且在40-320μg／kg／d劑量范圍內(nèi)抑制率為25-50％日韩综合无码视频一区二区三区，給藥劑量與腫瘤抑制率呈現(xiàn)一定量效關(guān)系轻点肏我在线观看91；酪絲亮肽體外對人肝癌BEL-7402細(xì)胞生長有一定的抑制作用，在作用72hrs時各濃度酪絲亮肽對腫瘤細(xì)胞的抑制作用較24hrs亚洲欧美日本一区二区综合、48hrs三级片AAA、96hrs明顯，其中濃度為100μg／ml時抑制率達19.36％中文字幕中日韩欧美一区；酪絲亮肽體內(nèi)外均能增強小鼠腹腔巨噬細(xì)胞對腫瘤細(xì)胞的殺傷：體外作用中巨噬細(xì)胞對BEL-7402精品在线视频99、B16-F10的殺傷功能明顯增強，與效應(yīng)細(xì)胞對照組相比有顯著性差異(P＜0.05)殺傷率分別達到35.58％特级欧美AAAAAA视、61.2％av中文在线一级片；體內(nèi)作用中巨噬細(xì)胞對BEL-7402、B16-F10的殺傷功能明顯增強欧美激情亚洲日韩专区，與生理鹽水對照組相比有顯著性差異(P 国产精品一级黄色毛片。05)，殺傷率分別達到21.39%A片免费观看在线播放、47.63%淫荡少妇一级性爱视频；裸鼠腹腔巨噬細(xì)胞經(jīng)酪絲亮膚作用后對BEL一7402、B 16一F10殺傷功能明顯增強亚洲黄色12级视频，與生理鹽水對照組相比有顯著性差異(P.05)伊甸园永久地址在线2022，最高殺傷率分別達到32.86%、73.07%国内黄片免费看；酪絲亮膚能增強單核巨噬細(xì)胞系統(tǒng)的吞噬功能亚洲欧美性爱一区，吞噬指數(shù)與生理鹽水組比較有顯著性差異(P.05)；酪絲亮膚體外作用能促進小鼠腹腔巨噬細(xì)胞分泌合成細(xì)胞毒效應(yīng)分子IL一lp欧美性爱肛交在线观看、TNF一Q和NO可以直接免费看黄色视频的网址，與效應(yīng)細(xì)胞對照組相比有顯著性差異(P.05)；酪絲亮膚體內(nèi)作用能促進小鼠腹腔巨噬細(xì)胞分泌合成細(xì)胞毒效應(yīng)分子IL一lp强奸人人操、TNF一Q和NO免费欧洲毛片在线视频，與生理鹽水對照組相比有顯著性差異(P.05)；酪絲亮膚能促進鼠巨噬細(xì)胞株R戌W264.7分泌合成IL一1p和NO国内精品久久久麻豆精品，IL一1日日韩色情国内自拍网页、NO水平分別在酪絲亮膚作用24hrs、12hrs時達到高峰国产三级片插逼视频，酪絲亮膚單獨應(yīng)用能提高巨噬細(xì)胞的分泌合成功能毛片黄色网站视频在线播放，而且酪絲亮膚能與LPS協(xié)同作用刺激巨噬細(xì)胞的細(xì)胞毒效應(yīng)分子分泌合成。

Considering the merits of in vivo studies in biotransformation i. e. activation or inactivation, protein-binding such as sex hormone binding protein , toxicokinetics, and more evaluative in hazard identification and risk assessment, we selected a battery of short-term and long-term in vivo studies, including uterotrophic assay in weaning mice and ovariectomized adult mice, an in vivo multiple endpoints assay, and a gestational and lactational exposure assay to identify the estrogenic effects of endosulfan on the offspring.

考慮到體內(nèi)試驗在代謝轉(zhuǎn)化黄色a级片又大又爽又黄又好看的、血漿蛋白如性激素結(jié)合蛋白的結(jié)合色哟哟在线精品、代謝動力學(xué)等方面比體外試驗優(yōu)越，在危害鑒定和危險度評定中更有價值欧美在线观看精品一区二区三区，我們選擇了多項體內(nèi)試驗亚洲资源一区二区在线观看，包括對幼年小鼠和卵巢切除成年小鼠的子宮增殖作用国产片免费、對成年去勢小鼠的體內(nèi)多終點觀察以及大鼠孕期和哺乳期接觸對雄性子代生殖系統(tǒng)結(jié)構(gòu)和功能的改變，即去雄性化或雌性化作用等国产三级片一区,二区在线，來驗證硫丹的體內(nèi)雌激素樣作用超碰免费在线站长工具。

Here we investigate the effect of 370G on the metabolism of bone in vivo and in vitro. 3 week-old S.D. male rats were used. Implantation of cannula (22G) was done from the posteriolaterial side into the proximal tibial metaphysis in limbs of the rats.

本論文則探討370G對於骨骼代謝的影響激情亚洲小说网。in vivo方面只久久久久久久久久久，本篇實驗使用3周大的公鼠，利用兩種動物模式來評估370G對其骨骼代謝的影響欧美中韩一区二区。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本實驗主要由以下三部分組成：一日韩系列精品自拍视频在线观看、n-HA/PA66 復(fù)合材料在動物體內(nèi)、體外的生物相容性及生物安全性評價二人妻人操人人操人人插、n-HA/PA66 復(fù)合材料植入動物體內(nèi)的生物活性及近期對機體鈣欧美熟女性爱图、磷代謝影響的實驗研究三、網(wǎng)孔 n-HA/PA66 復(fù)合材料作為支架修復(fù)兔橈骨節(jié)段缺損的動物實驗研究主要研究目標(biāo)及方法如下：參照 GB/T16886.5-1997-ISO 10993-5：1992《醫(yī)療器械生物學(xué)評價細(xì)胞毒性試驗體外法》之評價標(biāo)準(zhǔn)和要求日韩成人精品视频自拍，采用規(guī)定的 L929 細(xì)胞(小鼠結(jié)締組織成纖維細(xì)胞)欧美一区二区91，分別經(jīng)直接接觸和材料浸提液與細(xì)胞共培養(yǎng)等方式對 n-HA/PA66 復(fù)合材料進行細(xì)胞毒性測試，采用細(xì)胞形態(tài)觀察法觀察兩種細(xì)胞各組在 24h亚洲一级毛片免费看、48h精品a在线不卡、72h、5 天后各時相點的細(xì)胞形態(tài)學(xué)變化国产精品一级黄录像，并在顯微鏡下照相www欧美黄色网站，從而對細(xì)胞與材料的生物相容性進行定性評價；同時采用細(xì)胞生長抑制法小黄片在线播放情侣，以酶標(biāo)儀定量測定評價各組 1A片ねんね视频，2，426uuu亚洲日韩欧美，7 天 L929 細(xì)胞的相對增殖率91无码精品xyz，以定量測定并判別材料對細(xì)胞的毒性程度。

There were six themes in this study. First of all, the geometry of in-vivo human pinna and ear canal was measured non-invasively. The 3D model of pinna and ear canal was reconstructed and then measured based on the image processed by HRCT. Second, the 2D curvature of in-vivo human ear canal was measured non-invasively. The angles and curvatures of superior and inferior walls of first and second bends were measured. The 2D curvature of ear canal was computed by sine and cosine laws. Third, the in-vivo human ear canal was reconstructed by RE. The distribution of sound field was analyzed by FEM. The data obtained in this study can be the reference for clinicians and audiologists. Fourth, the 3D model of earmold of hearing aids, including the ear shell and the housing, was reconstructed by RE based on the image of in-vivo human ear canal obtained by CT.

本研究主題其四在线观看黄A片免费网站免费，對於聽力受損之患者往往皆須選配助聽器幫助其放大聲音黄色轻点疼视频网站，其中耳模是助聽器的外殼，也是接觸到耳朵的儀器国产青榴视频A片在线观看不卡成，而耳道型助聽器系位於耳甲艇及耳甲腔延伸至耳道第一彎道處男女视频在线免费看，在制作耳模必須利用灌模方式來取得緊配之耳模，其中耳模在助聽器的使用過程中欧美刺激a片影院，常因耳模松脫无码亚洲电影在线看，無法與人耳道緊密配合而造成漏音，因此A级黄色片在线免费观看，利用活體人外耳道電腦斷層影像A片vi视频，重建耳道型助聽器耳模之三維立體模型影像国产综合亚洲欧美日韩一区，其中耳模包括了耳殼及耳模內(nèi)部空間，并進行幾何外型量測精品国产亚洲，探討耳道型助聽器之體容積大小999国产在线视频，也提供了制作耳模時快速、精確度高成视频年人黄网站免费视频高潮、減少翻模及修模次數(shù)玖玖草视频，可減少誤差率之方法。

The test groups were drenched orally with different dosages of compound Radix scutellariae granules. After being drenched orally for ten days, their thymus-index and spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 of serum were defermined, and experiments in vivo were done. The mice-spleen's lymphocytes which were cultured in vivo were added with different concentrations of compound Radix scutellariae granules. The lymphocytes translation stimulate indexes were measured. The ability of licking up neutral red of the peritoneal macrophages cultured in vivo was measured in the groups.

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In vivo footprinting is a method of studying DNA-protein interaction. It can reflect the authentic status of DNA-protein in vivo, and can also detect the change of DNA conformation. The introduction of Ligation-Mediated PCR greatly improves the sensitivity and specificity of in vivo footprinting study, and has facilitated the executation of in vivo footprinting in the regulation of eucaryotic gene expression.

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To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

為了解豬皮膚成纖維細(xì)胞PERV在體外和體內(nèi)的感染性，通過建立豬皮膚成纖維細(xì)胞系wwwww在线国产，將所建細(xì)胞系與人胚胎腎293細(xì)胞體外共培養(yǎng)国产精品熟女30p，并移植于嚴(yán)重聯(lián)合免疫缺陷鼠皮下進行豬皮膚成纖維細(xì)胞PERV的體外和體內(nèi)感染性實驗，結(jié)果表明欧美在线视频一区18p，豬皮膚成纖維細(xì)胞與人胚胎腎細(xì)胞共培養(yǎng)過程中99riav6，豬內(nèi)源性逆轉(zhuǎn)錄病霉感染人胚胎腎細(xì)胞，進一步證實和拓寬了豬細(xì)胞PERV感染人細(xì)胞的范疇国产精品99久久久久久久www；豬皮膚成纖維細(xì)胞移植SCID鼠皮下后尤物网站，導(dǎo)致SCID鼠發(fā)生豬細(xì)胞微嵌合(78.57%)和PERV在體內(nèi)感染(85.71%)并且波及遠(yuǎn)離移植部位的多種組織或器官，但是并未檢測出SCID鼠組織中表達PERV env RNAA级黄色视频免费。