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Content of Chla,β-carotene and zeaxanthin of S. platensis grown in Se decreased, violaxathin, fucoxanthin, 19'-hexanoyloxyfucoxanthin and peridinin increased, Chla and Chlb of D. salina also decreased, but carotene increased, all pigments of D. zhanjiangensis increased except violaxanthin and 19'-hexanoyloxyfucoxanthin decreased. Effect of Ge on pigments of microalgaes were different in different algaes: Chla,β-carotene and zeaxanthin of S. platensis decreased, three other carotene increased, in D. salina, violaxanthin and lutein decreased, violaxathin, fucoxanthin and 19'-hexanoyloxyfucoxanthin increased, all pigments of D. zhanjiangensis increased except fucoxanthin and peridinin decreased; Chla,β-carotene and violaxanthin of Chlorella sp increased, but fucoxanthin and peridinin decreased.

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24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治療腦缺血12小時(shí)組缺血腦組織TGF-β1光密度值較缺血12小時(shí)模型組無(wú)顯著性差異(p>0.05)。本實(shí)驗(yàn)結(jié)果發(fā)現(xiàn)呦呦网址,與單純?nèi)毖?2小時(shí)美女黄色被插视频、24小時(shí)大鼠相比,藥物干預(yù)組缺血側(cè)大腦皮層TGF-β1免疫反應(yīng)明顯增強(qiáng)91黄色大片,計(jì)算機(jī)輔助圖像半定量分析兩組間缺血側(cè)大腦皮層TGF-β1免疫陽(yáng)性細(xì)胞數(shù)差異非常顯著男插女黄色视频在线观看网址。說(shuō)明藥物對(duì)局灶性腦缺血損傷可能有一定保護(hù)作用91AⅤ看片,其機(jī)制可能與誘導(dǎo)腦細(xì)胞TGF-β1合成增加有關(guān)。然而人人操人人艹人人射,藥物調(diào)控TGF-β1表達(dá)日本视频一区在线观看,其促TGF-β1表達(dá)增強(qiáng)的機(jī)制可能是這樣的:腦缺血時(shí)TGF-β1mRNA表達(dá)增加可能與缺氧誘導(dǎo)神經(jīng)和膠質(zhì)細(xì)胞刺激生長(zhǎng)因子表達(dá)有關(guān)。

12H treatment group compared with 12h model group: the density of light of HSP70 of the Gardenoside group is not different and which of it of TGFβ〓 increased with the model group; the density of light of the HSP70 of the Baicalin group in not different and which of the TGF-β〓 is no different with the model group, the density of HSP70 of the Concha Margatitifera Usta is not different and which of TGFβ〓 increased than the model group; the density of the HSP70 of the Cholic Acid group is no different and the density of TGF-β〓 increased than the model; the density of the light of HSP70 of the Hefang Group increased and which of TGF-β〓 increased than the model group.

合方治療腦缺血12小時(shí)組缺血腦組織TGF-β1光密度值較缺血12小時(shí)模型組明顯升高(p<0.01)精品黄色A片在线观看。大鼠局灶性腦缺血24小時(shí)模型組缺血腦組織TGF-β1光密度值含量較正常組明顯增高(p<0.01)女孩小穴自慰网站。珍珠母治療腦缺血24小時(shí)組缺血腦組織TGF-β1光密度值較缺血24小時(shí)模型組明顯升高(p<0.01)。膽酸治療腦缺血24小時(shí)組缺血腦組織TGF-β1光密度值較缺血24小時(shí)模型組明顯升高(p<0.01)国产一级特黄A片A视频。梔子苷治療腦缺血24小時(shí)組缺血腦組織TGF-β1光密度值較缺血24小時(shí)模型組無(wú)顯著性差異(p>0.05)免費一級毛片。黃芩苷治療腦缺血24小時(shí)組缺血腦組織TGF-β1光密度值較缺血24小時(shí)模型組明顯升高(p<0.01)。

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The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P<0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P<0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P>0.05). Enzymepreparation significantly increased (P<0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P>0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

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It is shown from the assessment results that (1) both the TN and TP fluxes discharged from the Changjiang River to the Changjiang River estuary sea area increased continuedly, the DIN concentration in the sea areas increased continuedly, and the Si concentration decreased continuedly, which resulted in the significant changes in N/P/Si ratios;(2) the chlorophyll a concentration in the sea areas increased continuedly, and the significant changes in community structure of phytoplankton occurred;(3) the species and biomass of benthos declined greatly, and the bottom oxygen-poor water area increased significantly;(4) the range and occurrence frequency of red tide events in the sea areas increased greatly, and the algal toxic infection events of mussel were frequently found in the sea areas.

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The Go moved backward with 1.35mm and upward with 1.90mm in group I, the gonial angle increased for 3.69°, and angle between palatal plane and mandibular plane was increased for 5.00°; In group II, the Go moved backward with 1.85mm and upward with 3.32mm , the gonial angle increased for 4.06°, and angle between palatal plane and mandibular plane was increased for 5.22°. Both in group I and II, there was a tread for clockwise rotation of mandibular corpus. The length of pterygomasseteric sling was mild increased due to the backward movement of Go.

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The increased amount of non--exchangeable NH4^+-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^+-N (P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively). NH4^+-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^+-N increased significantly when added (NH4)2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^+-N from (NH4)2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^+-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d (P=0.0434), but not significantly at 40 d and 60d (P=0.7378 and 0.5375). The increased amount of non-exchangeable NH4^+-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^+-N, the increased amount of non-exchangeable NH4^+-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用腦立體定位、核團(tuán)插管日韩黄色视频中文字幕、微量注射欧日一级黄片、多導(dǎo)睡眠描記等方法,研究丘腦網(wǎng)狀核(nucleus reticularis thalami自拍视频.com,RT)中γ-氨基丁酸(gamma-amino butyric acid 色色五月天社区,GABA)能神經(jīng)元、一氧化氮(nitrogen monoxidum操美国大逼,NO)能神經(jīng)元黄片看看、阿片肽類神經(jīng)遞質(zhì)、環(huán)一磷酸腺苷(cyclic adenosine monophosphate丁香欧美亚洲小说在线观看,cAMP)及中縫背核(nucleus raphes dorsalis26uuu欧美综合,DRN)至RT的5-羥色胺(5-hydroxytryptamine,5-HT)能神經(jīng)纖維投射對(duì)大鼠睡眠-覺(jué)醒周期的影響及其作用機(jī)制人人操大屁股。1 RT內(nèi)GABA能神經(jīng)元對(duì)大鼠睡眠-覺(jué)醒周期的影響1.1大鼠RT內(nèi)微量注射GABA合成關(guān)鍵酶抑制劑3-巰基丙酸(3-MP超碰青春娱乐网,5μg),注射當(dāng)天睡眠時(shí)間略有減少黄片免费进,第二日睡眠時(shí)間顯著減少一级视频a,覺(jué)醒時(shí)間明顯增多,第三亚洲色图欧美色图观看日本一道、四日睡眠和覺(jué)醒時(shí)間逐漸恢復(fù)至正常.精品国产首页,。1.2大鼠RT內(nèi)微量注射GABA受體激動(dòng)劑GABA( 1.0μg)后欧美A片观看视频,與生理鹽水組比較,睡眠時(shí)間增加在线视频播放A片,覺(jué)醒時(shí)間減少有没有网站免费播放黄色一级片;而RT內(nèi)微量注射L-谷氨酸(glutamic acid, L-Glu老女偷偷伦偷偷啪, 0.2μg)后日韩乱码一区二区三区,睡眠時(shí)間減少,覺(jué)醒時(shí)間增加国产激情自拍视频在线观看;RT內(nèi)微量注射GABAA受體阻斷劑荷包牡丹堿(bicuculline日韩成人网址播放器在线播放免费,BIC欧美老妇逼,1.0μg)后女生被操的喷水视频,睡眠時(shí)間減少,覺(jué)醒時(shí)間增加一级黄免费视频;RT內(nèi)微量注射GABAB受體激動(dòng)劑氯苯氨丁酸(baclofen不卡国产在线,BAC,1.0μg)后欧美黑白配性爱视频,產(chǎn)生了與GABA相似的促睡眠效果www欧美操逼视频。2 RT內(nèi)NO能神經(jīng)元對(duì)大鼠睡眠-覺(jué)醒周期的影響2.1大鼠RT內(nèi)微量注射NO的前體L-精氨酸(L-Arg,0.5μg)后美女黄片视频免费观看,與生理鹽水組對(duì)比国产综合精一区二区在线观看,睡眠時(shí)間略有減少,但無(wú)顯著性意義欧美精品热视频在线观看;而RT內(nèi)微量注射NO的供體硝普鈉(Sodium Nitroprusside黄片A片视频免费在线观看,SNP,0.2μg)后可明顯增加覺(jué)醒時(shí)間人看人爽人操人摸人日人碰,縮短睡眠時(shí)間最新黄色视频在线播放;微量注射一氧化氮合酶抑制劑L-硝基精氨酸(L-arginine,L-NNA欧美精品A∨欧洲精品,2.0μg)后妇女人人操人人干,引起睡眠時(shí)間增多,覺(jué)醒時(shí)間減少色噜噜午夜午伦视频一区二区。2.2大鼠RT內(nèi)同時(shí)微量注射L-NNA(2.0μg)和SNP(0.2μg)后與L-NNA組比較發(fā)現(xiàn)SNP逆轉(zhuǎn)了L-NNA的促睡眠作用欧美成性互相;RT內(nèi)同時(shí)微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,與L-NNA(2.0μg)組比較發(fā)現(xiàn)L-Arg可以增加覺(jué)醒而縮短睡眠一级做性色a爰片久久毛片体验区,其促覺(jué)醒作用未能被NOS的抑制劑L-NNA所逆轉(zhuǎn)色色网五月。3 RT內(nèi)阿片肽對(duì)大鼠睡眠-覺(jué)醒周期的影響3.1大鼠RT內(nèi)微量注射硫酸嗎啡(morphine sulfate,MOR亚洲人成A片在线观看尤物,1.0μg)后與生理鹽水組對(duì)比性一级录像视频网站免费大全,睡眠時(shí)間減少而覺(jué)醒時(shí)間增加欧美综合色婷婷欧美综合五月; RT內(nèi)微量注射阿片肽受體拮抗劑鹽酸納洛酮(naloxone hydrochloride,NALav黄色在线观看免费,1.0μg)后與生理鹽水組比較国产一级激情免费视频,睡眠時(shí)間增加而覺(jué)醒時(shí)間減少。3.2大鼠RT內(nèi)同時(shí)微量注射MOR(1.0μg)和NAL(1.0μg)后1024色婬免费视频,與生理鹽水組對(duì)比一级无遮挡真人毛片A片,原有的MOR促覺(jué)醒效果和NAL的促睡眠效果都沒(méi)有表現(xiàn)。4 RT內(nèi)環(huán)一磷酸腺苷信使對(duì)大鼠睡眠-覺(jué)醒周期的影響大鼠RT內(nèi)微量注射cAMP(1.0μg)后與NS(1.0μg)組比較A级黄色网站免费看,睡眠時(shí)間增多而覺(jué)醒時(shí)間減少国产操av;RT內(nèi)微量注射亞甲藍(lán)(methylene blue,MB亚洲欧美天堂在线,1.0μg)后丝袜AV观看一区,與NS組比較,睡眠時(shí)間增多而覺(jué)醒時(shí)間減少国产精品亚洲精品91。5中縫背核投射到丘腦網(wǎng)狀核的5-羥色胺能神經(jīng)纖維對(duì)大鼠睡眠-覺(jué)醒周期的影響5.1大鼠DRN內(nèi)微量注射L-Glu(0.2μg)国产免费黄片视频,同時(shí)在雙側(cè)RT內(nèi)微量注射NS (1.0μg)后,與對(duì)照組(DRN和雙側(cè)RT注射NS1313久久午夜精品片, 0.2μg)比較国产一级做,睡眠時(shí)間減少,覺(jué)醒時(shí)間增多www.bjct-laser.com;大鼠DRN內(nèi)微量注射L-Glu(0.2μg)人人摸,人人看,同時(shí)在雙側(cè)RT內(nèi)微量注射二甲基麥角新堿(methysergide, MS新超碰免费男人网站, 1.0μg )后亚洲日本欧美理论片在线观看,與對(duì)照組(DRN注射L-Glu 0.2μg,雙側(cè)RT注射NS 1.0μg)比較色欲天天婬色婬香综,睡眠時(shí)間增多欧美一级专区,覺(jué)醒時(shí)間減少。5.2大鼠DRN內(nèi)微量注射對(duì)氯苯丙氨酸(p-chlorophenylalanine黄色成人网站在线观看,PCPA国产A级高清视频毛片,10μg),同時(shí)在雙側(cè)RT內(nèi)微量注射NS (1.0μg)后国产精品区最新视频,與對(duì)照組(DRN和雙側(cè)RT注射NS国产盗摄一区, 1.0μg)比較,睡眠時(shí)間增多网址在线观看国产日韩视频,覺(jué)醒時(shí)間減少日韩乱伦字幕中文一区;大鼠DRN內(nèi)微量注射PCPA(10μg),產(chǎn)生睡眠增多效應(yīng)后,在雙側(cè)RT內(nèi)微量注射5-羥色胺酸(5-hydroxytryptaphan 色色色五月天久鬼久, 5-HTP日本很爽免费网站, 1.0μg )后,與對(duì)照組(DRN注射PCPA 10μg偷拍福利视频网站,雙側(cè)RT注射NS 1.0μg)比較欧美在线视频干美女,睡眠時(shí)間減少,覺(jué)醒時(shí)間增多free性video丝袜极品。

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