The cocultures were incubated at 37 in 5% CO2 in air in DMEM-LG medium supplemented with 10% FBS.

已知與單核系定向分化有關(guān)的細(xì)胞因子有GM一cSF和M一cSF。

METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10^8/L were incubated in 12-well plate with a coverslip.

全骨髓法體外分離培養(yǎng)黄页网址在线、純化大鼠骨髓間充質(zhì)干細(xì)胞免费网站在线观看人成视频，傳至第4代時(shí)日韩精品第四页在线观看视频，按4×10^8 L^(-1)密度接種于置有蓋玻片的12孔板內(nèi)亚洲你懂的在线视频，制備細(xì)胞爬片国内精品色。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法體外分離培養(yǎng)人骨髓間充質(zhì)干細(xì)胞，傳至第3代以1×108 L-1密度接種至預(yù)置蓋玻片的6孔培養(yǎng)板中特级特黄色视频，設(shè)立3組：成骨誘導(dǎo)組添加原代培養(yǎng)液后插入女生视频，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油丁香五月婷、50 mg/L維生素C的成骨誘導(dǎo)培養(yǎng)基国产欧美综合在线视频；成骨+瘦素誘導(dǎo)組添加成骨誘導(dǎo)培養(yǎng)基后，再加入50 nmol/L瘦素二区视频在线；空白對(duì)照組僅加入含體積分?jǐn)?shù)為10%胎牛血清的LG-DMEM培養(yǎng)液5060网全毛A片。

Cultured PC12 cells were incubated with Fluo 3-AM working solution (the final concentration of Fluo 3-AM is 5μmol/L) at 37℃for 45min, followed by 3 times washes with the culture medium. The cultured neurons grown on glass coverslips were placed in a cell chamber on the stage of an inverted microscope.

多種應(yīng)激刺激通過細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)途徑使CREB第133位的絲氨酸磷酸化而激活，活化的CREB直接或間接激活相關(guān)基因的轉(zhuǎn)錄又硬又黄又粗又大又爽的视频日韩，進(jìn)而表述某些蛋白分子以及某些神經(jīng)營養(yǎng)因子等欧美特黄色视频，進(jìn)而調(diào)節(jié)神經(jīng)元在應(yīng)激性損傷后再生、存活及修復(fù)等中文字幕欧洲有码无码。

Incubated with cytochalisin B for 4 hours, F-actin bunddles disrupted, confluent ECs monolayer has a complete detachment from the coverslips. So, there is a suggestion that the F-actin in ECs is essential for endothelial adherence under shear stress.

Northcrn雜交結(jié)果表明外源性纖維連接蛋白有促進(jìn)EC肌動(dòng)蛋白表達(dá)的作用A片一级免费视频，結(jié)合F-actin染色及FN受體染色結(jié)果，本文認(rèn)為用纖維連接蛋白作為細(xì)胞種植的促粘附物質(zhì)有助于內(nèi)皮細(xì)胞粘附結(jié)構(gòu)的形成狼友视频在线免费g观看。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

實(shí)驗(yàn)方法1分離、純化免费人成黄页在线观看、培養(yǎng)亚洲激情图片、鑒定兔BMSCs并以SPIO以25μg Fe/ml培養(yǎng)液濃度標(biāo)記，對(duì)標(biāo)記后不同時(shí)間的細(xì)胞行普魯士藍(lán)染色和臺(tái)盼藍(lán)拒染后顯微鏡觀察黄色网址手机在线观看亚洲无码。2將不同細(xì)胞濃度標(biāo)記細(xì)胞管(1×104/ml欧美一级高潮片在线观看、5×104/ml、1×105/mlwww中国黄色一级片a、5×105/ml怡红院wWw在线观看、1×106/ml美女张开腿让男生桶、5×106/ml)，以不同掃描序列T1WI欧美精品一级一区，T2WIaⅴ色图，T2*WI(GRE進(jìn)行MR成像，再選擇相同細(xì)胞濃度組(5×104/ml极品国产盛宴在线观看免费、1×105/ml)進(jìn)行不同時(shí)相MR成像色一级免费视频，并測(cè)量信號(hào)強(qiáng)度，進(jìn)行統(tǒng)計(jì)學(xué)分析毛片免费毛片一级JJJ毛片。3缺血再灌注腎損傷模型建立和處理在线AⅤ视频播放，然后將標(biāo)記和未標(biāo)記細(xì)胞(5×105個(gè))經(jīng)耳緣靜脈移植入家兔體內(nèi)(共16只：注入標(biāo)記細(xì)胞者10只，注入未標(biāo)記細(xì)胞者6只)18岁以下免费网站在线观看，兩組均于注射前黄色网站无码在线免费播放、注射后第0、1开心五月丁香婷婷国产在线A片、3美国无码精品、5、8天應(yīng)用MRI對(duì)移植細(xì)胞進(jìn)行活體示蹤并與腎臟組織切片對(duì)照欧美老人一级黄色视频，然后對(duì)收集的信號(hào)強(qiáng)度進(jìn)行統(tǒng)計(jì)學(xué)分析日韩在线观。

If tissue culture medium is incubated in dialyzers and then rat dorsal root ganglion cultures are exposed to this medium, characteristic changes occur.

EO殘留量主要是指EO滅菌后留在物品和包裝材料內(nèi)的EO和它的兩個(gè)副產(chǎn)品氯乙醇乙烷和乙二醇乙烷。

To test whether ethylene oxide retained in the dialyzers might be neurotoxic, tissue culture medium was incubated in the blood compartment of dialyzers.

如果組織培養(yǎng)基在透析器內(nèi)孵育a片在线视频免费，然后將鼠的背根神經(jīng)節(jié)（DRG培養(yǎng)物暴露于此培養(yǎng)基中国产在线观看无码免费Aa，則DRG的特異性變化會(huì)發(fā)生。

There were also conspicuous accumulations of membrane bound esicles, dense bodies, myeloid figures, and areas of esicular in Schwann cells. These changes were seen with dialyzer exposed medium or with medium containing patient serum but not with controls or with medium incubated in plate dialyzers.

這些變化見于使用了暴露于透析器的培養(yǎng)基的DRG禁止18岁黄色网站，或使用含有病人血清的培養(yǎng)基的DRG最新先锋最新网址，但變化不發(fā)生于使用對(duì)照用組織培養(yǎng)基或者在平板透析膜中孵育的DRG。

Electron microscope findings were compared between DRG grown in control medium containing 10% calf serum, DRG incubated in the same medium exposed to the blood compartment of capillary flow or plate dialyzers and DRG grown in medium supplemented with 10% dialysis patient serum.

對(duì)下列基質(zhì)中生長(zhǎng)的DRG的電鏡發(fā)現(xiàn)進(jìn)行比較：即DRG在含有10%牛血清的對(duì)照基質(zhì)中生長(zhǎng)欧美日韩你懂的、然后在暴露于毛細(xì)流血隔室或平板過濾膜（ blood compartment of capillary flow or plate dialyzers）的相同基質(zhì)中孵育日韩综艺精品视频在线；DRG生長(zhǎng)于加有10%血透病人血清的組織培養(yǎng)基內(nèi)。