After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

將獲得的DC與用 E-花環(huán)分離臍血單個核細胞得到的T淋巴細胞共同培養(yǎng)3 d操操日日，然后分為兩組欧洲黄片毛片，實驗組2繼續(xù)與不同比例的HO9810細胞共同培養(yǎng)3 d后獲得細胞毒T淋巴細胞在线国产黄色网址，對照組2除加入HO9810外亚洲特黄毛片一级无码，還加入了無關(guān)細胞找点黄片看看听听叫床，用四甲基偶氮唑藍法檢測其對HO9810細胞的抑制作用中文字幕乱伦91。

Filtrate of cultured dermatophyte and candia albican was incubated with human keratinocyte,the level of growth factor and the effect were comparatively analysed.

取淺部真菌和白念珠菌培養(yǎng)濾液與原代培養(yǎng)人類角質(zhì)形成細胞孵育，比較表皮因子值及其對角質(zhì)形成細胞生長的作用国产iGAO在线。

Extensor digitorium longus and soleus muscles which represent two types of muscle fiber were studiedd. Results In the early postburn period as well as in septic states,the lactate production of muscle cells was significantly elevated as compared to the normal controls even though the muscles were incubated in a fully oxygenated media.The levels of aerobic glyoclysis,as well as its changes in postburn period and in septic states,vary depending on the difference in the fiber composition of the muscles.

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Every explant was pricked with a dissector at the center of one subregion before inoculated on MS+BA 1mg/L+NAA 4mg/L+2,4D 05mg/L medium and incubated in darkness for 7 days,then transfered to MS+BA 1mg/L medium.85% of the explants regenerated somatic embryos directly around the prick after 40 days.

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The two lobes of thyroid gland from each mouse were separated into two groups and incubated in Earle's medium.

在相同的培養(yǎng)條件下www.hljwlb.com，大鼠甲狀腺過氧化物酶活性，實驗組比對照組增高了約三倍少妇漫画爆乳同人志全彩。

The oxidative burst modulators were added and cells incubated for an optimized period of time prior to addition of the elicitor, unless stated differently.

氧化爆裂調(diào)制的補充和細胞的培養(yǎng)優(yōu)化之前很長一段時間国产亚洲无线一区二区，增加了 elicitor ，除非表示不同的看法欧美一区二。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通過構(gòu)建真核表達質(zhì)粒pCMVCD 亚洲中文字幕区，限制性內(nèi)切酶消化鑒定后，采用Lipofectamine 2000 脂質(zhì)體介導(dǎo)法轉(zhuǎn)染新生大鼠室管膜下區(qū)神經(jīng)干細胞(Neural stemcells 超污污网站， NSCs)日韩精品网站日韩在线观看免费视频，G418 篩選陽性克隆国产视频自拍视频，加入不同濃度的52氟胞嘧啶(52Flourocytosine ， 52FC)日韩欧美操逼视频，MTT比色法測定NSCs的生存率国产AV真人一区二区凹凸。

EPC were harvested and incubated with vehicle or insulin (0.1, 1, 10 nmol/L).

胰島素對于心血管的生理功能具有重要的調(diào)節(jié)作用，但其對EPC影響的相關(guān)報道較少亚洲视频精品一区。

Methods S.mutans were incubated respectively in 0.5%, 1%, 2%, 4%, 8%,12%, 16% erythritol or xylitol culture medium under anaerobic conditions. The A and pH value of the mediums were measured at 0, 2, 4, 6, 8, 10, 12, 18, 24 hours, following the profile plots by SPSS.

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Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基質(zhì)細胞（1×105·mL-1）三維膠原凝膠表面添加提純的不同濃度綠膿桿菌外毒素A（0.118禁黄网站禁片免费观看在线、1.0和10 mg·L-1）后免费黄色网址1，放入無血清MEM培養(yǎng)液中37℃培養(yǎng)24 h，采用光學(xué)顯微鏡觀察培養(yǎng)的兔角膜基質(zhì)細胞形態(tài)學(xué)變化色黄色免费在线，采用酶聯(lián)免疫吸附法檢測損傷的兔角膜基質(zhì)細胞釋放的乳酸脫氫酶含量国产黄色网站播放。