The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

將兔抗IgG 作用于Alexa 488金黃色葡萄球菌的活性顆粒Aa黄色网址，后與實驗細胞(上述4種)孵育1小時无码作爱高潮视频，再用Alexa 633羊抗兔IgG定位日韩中文激情无码迅雷下载，以區(qū)別活性顆粒是被吞噬的還是位于細胞外的在线国产91小视频。

The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

將兔抗IgG 作用于Alexa 488金黃色葡萄球菌的活性顆粒，后與NTM細胞孵育1小時天天日天天爽天天要Av，再用Alexa 633羊抗兔IgG定位日日操天天干天天，以區(qū)別所吞噬的位于細胞外的活性顆粒。

Fibroblasts were passaged with 0.25% trypsin.After 24 and 48 hours incubation,MTT was used to measure the cell proliferation.

取第3代MCL細胞可以进的黄网站，培養(yǎng)24免费一级黄色操B网站、48 h后，采用二甲基噻唑二苯基四唑溴鹽法測定細胞增殖欧美精品综合第一页。

METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry.

日本大耳兔骨髓間充質(zhì)干細胞進行體外培養(yǎng)擴增后欧美日韩羞羞视频，通過pCDNA3.1質(zhì)粒將堿性成纖維細胞生長因子基因轉(zhuǎn)染至生長狀態(tài)良好的骨髓間充質(zhì)干細胞，并將轉(zhuǎn)染后的細胞接種于制備好的豬小腸黏膜下層上91自产自拍国际，進行體外聯(lián)合培養(yǎng)2022www.黄色网，構(gòu)建組織工程皮膚。

Objective To know if MCF-7 breast cancer cell line has breast cancer stem cell,and change of stem cell pool in diverse incubation circumstance.

以及在不同的培養(yǎng)條件下人人操国产在线，干細胞池的變化情況日本黄色片AAAAAA。

Methods The chemical hypoxia was induced by incubation of U251 cells with cobalt chloride.

以氯化鈷模擬缺氧，采用RT-PCR欧美一级A片在线播放器、免疫細胞化學(xué)方法分別檢測缺氧誘導(dǎo)因子-1α和血管內(nèi)皮生長因子mRNA和蛋白的表達国产第一页久久亚洲欧古代黄片，雞胚絨毛尿囊膜模型檢測缺氧時膠質(zhì)瘤細胞血管生成情況。

The results showed that the amount of nitrogen mineralized from the green manure added to soil was corelated with the effective cumulative temperature i.e.y=k[(T—15)D]~n in which y is the amount of nitrogen mineralized, D the number of days, T the actual temperature during incubation and k and n constants.

結(jié)果表明99综合色，在20—30℃淹水培養(yǎng)條件下一级一级一级一级XXXX，加入土壤中的綠肥氮礦化率與有效積溫(T—15)D的關(guān)系，符合y=K[(T一15)D]~n經(jīng)驗公式的規(guī)律盗摄偷窥。

Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株為試驗菌株免费小黄片无码，當培養(yǎng)基中甘油初始濃度為1.5%，并且在發(fā)酵60h時補加1.0%的甘油亚洲白浆一区二区三区四区，搖瓶發(fā)酵的效價可達2668μg/ml黄色免费cnm，較出發(fā)菌株(318μg/ml)提高了7.4倍。

After 72 hours incubation, the chondrocytes were digested into single cell suspension. Then they were incubated in the 96-well culture plate with the density of 1×10^8 L^(-1), 100 μL per well and six wells for per group.

白細胞介素1β加入量為5μg/L日本h纯肉无遮掩3d动漫，培養(yǎng)72h將實驗用細胞消化傳代后制成單細胞懸液一级女人18片毛片免费视频辶，以1×10^8L^的密度接種于96孔培養(yǎng)板，100μL/孔在线一级毛片免费视频，每組6孔国产A级强奸黄片。

Biodegradation and degradation kinetics of anion-surfactant in the anaerobic water of a representative inlet of Lake Dianchi under different incubation conditions were studied by the'river die-away'test method.

LAS 的結(jié)構(gòu)與生物降解性的關(guān)系[10 国产人碰人摸人爱视频，11 ] ；②利用馴化培養(yǎng)的高效菌株來降解廢水中高濃度的LAS[12～14 ] 啊啊啊无码网站在线观看；③ LAS 在水處理系統(tǒng)中的生物降解[15～17 ] 久久99国产综合精品第二页。