When the inoculative density of MSCs is l×l0~5cells/ml and the microcarrier Cytodex-3 concentration is 10mg/ml, MSCs cultivated in RWV for five days can expand more than nine times but only four times in T-fiask in one generation. Moreover, the culture parameters show that the media pH value in RWV is 7.4-7.6, and the glucose content is lower but the lactic acid content is higher in RWV than in T-flask.

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Meanwhile, the optimal use level of auxiliary rice is 37％; pH5.2～pH5.4 for the best saccharification, pH6.0～pH6.3 for the best gelatinization; 90 minutes for boiling; addition of 2kg hops the first time, 9kg the second time, and 7kg the third time; inoculative yeast 7％; the highest fermentation temperature 12℃ and the highest pressure 0.12MPa.

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