Isolates from watermelon-growing areas were classified into 3 races like race 0, race 1 and race 2 which were 8 (17.4), 30 (65.2), and 8 (17.4%), respectively. Pathogenecity of race 0 isolates are weakest which only make Sugar Baby susceptible; isolates of race 1 are intermediately pathogenetic, they make Sugar Baby and Charleston susceptible Gray but Calhoun Gray resistant; isolates belonging to race 2 possess the strongest pathogenecity, which make 3 hosts susceptible. Isolates of race 0 mainly distribute over the central and southern Hebei province, whereas race 2 cover the middle and north of Hebei, isolates of race 1 exist in the whole watermelon-growing areas in Hebei province.

根據(jù)鑒別寄主對供試菌系的抗感反應(yīng)，將46個西瓜枯萎病菌系劃分為0號国产三级激情在线视频、1號和2號3個不同的生理小種，分別包括8，30和8個菌系国产AV国片精品一区二区，占供試菌系的17.4%、65.2%和17.4%人人插人人做；0號生理小種菌系致病性最弱爱爱免费网站欧美，僅使品種Sugar Baby感病，以冀中和冀南居多一二三区无卡高清视频在线播放；1號生理小種菌系致病力中等亚洲毛片不卡88AV在线，使鑒別寄主Sugar Baby和Charleston Gray感病，而使Calhoun Gray抗病亚洲午夜福利电影，分布在整個河北省西瓜種植區(qū)性交毛片视频免费看；2號生理小種的菌系致病力最強，使3個鑒別寄主均能感病欧美一区欧美一区，主要分布在冀中和冀北日韩孕妇自拍视频直播在线观看网站。

In 42 ACME-arcA-positive isolates, 8 isolates harbored SCCmec V, 8 isolates harbored class C1 mec complex and ccrAB_, 22 isolates harboring class C1 mec complex and 4 isolates harboring class C2 mec complex were negative for all known ccr allotypes.

本研究在ACME-arcA基因陽性菌株中發(fā)現(xiàn)整合酶基因ccr的一種新亞型ccrAB_(SHP.42株ACME-arcA基因陽性菌株，8株攜帶V型SCCmecA级毛片视频，8株攜帶C1型mec基因復(fù)合體和ccrAB_少妇AA毛片在线播放，剩余26株攜帶C型mec基因復(fù)合體和未分型ccr。

Isolates CNR-1 and CNR-2 were from Brassica napus; CNA-1and CRW-1 from Rhododendron simsii and Oxalis corymbosa respectively. All isolates grew well in R-2 liquid medium and exhibited contractive movements. The colonies of all isolates were circinal and grain-like in solid medium. Through electron microscopy, all isolates exhibited helicity during their growth phase. All isolates could pass through a 0.22 μm filtrate membrane and resist to penicillin (2000 U/mL). They must grow in medium with serum.

這4株螺原體在R-2液體培養(yǎng)基中生長良好日本熟女强奸乱码，都能通過孔徑為0.22 mm的微孔濾膜国产情侣免费在线视频；在R-2固體培養(yǎng)基上呈圓形或顆粒狀菌落，菌落直徑約50~600 mm99热这里只有精品久久；在生長的某個階段可呈典型的螺旋狀桃花视频日本高清视频，菌體直徑為37.04~370.40 nm，長度約0.89~11.88 mm九色自拍视频在线观看；它們都能利用葡萄糖作為碳源不卡a片免费在线观看，不能利用尿素黄的视频黄的网站。；在不含胎牛血清的R-2培養(yǎng)基中，它們都不能生長一级a性色生活片毛片黄长官一；菌株CNR-1国产十八禁黄无遮禁视频免费、CNA-1能強烈代謝精氨酸，而CNR-2和CRW-1不能代謝精氨酸可以直接看黄色视频的网站。；在氨芐青霉素鈉濃度高達2000 U/mL的R-2培養(yǎng)基中好大好深好猛好爽好湿视频，分離菌株生長良好。

The results showed:among the 7 isolates, five isolates of HY3欧美日韩精品二区在线、GY1－3超碰在线19、ZJ1－1、HP1亚洲国产精品专区、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts色aaa、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

結(jié)果顯示：7個菌株中，其中5個菌株HY3国产午夜福利不卡在线观看、GY1－3亚洲综合在线图片、ZJ1－1、HP1欧洲最大黄色网站、FC3的菌落形態(tài)相同：不規(guī)則形狀黄片vs毛片、流體、粘性97国产精品免费视频、不透明国产嫖娼妓院视频网站、表面光滑；另外2個菌株93B男女爱爱免费超爽大片黄、GN1菌落形態(tài)相同：不規(guī)則形狀亚洲视频二区三区、干燥、不透明91av色片在线、表面粗糙亚洲激情黄色视频；用7個菌株接種剪根桉樹苗，發(fā)病情況非常明顯亚洲第一区色色色，而自來水對照實驗中桉樹苗卻不發(fā)膊宄槭悠怠；無EPS菌懸液培養(yǎng)桉樹剪根苗一级AAA毛片无码视频，發(fā)病率也很明顯污污黄色美女视频网站，但是相比原菌液，則發(fā)病率有不同程度的下降欧美性爱大屁股；從7個菌株中間篩選出來2個強致病性菌株和2個弱致病性菌株国产女人网站，用它們配制菌懸液培養(yǎng)桉樹苗，設(shè)置剪根和不剪根兩個處理，每個處理設(shè)置五個重復(fù)真人无码免费视频网页，數(shù)據(jù)分析結(jié)果顯示：對于剪根苗，上部和中部全网国产黄色视频、上部和下部的含菌量有顯著的差異欧美特黄一区二区三区观看在线网站，中部和下部含菌量差異不顯著；帶根苗特黄一级大片，上部99草在线视频观看、中部、下部含菌量彼此之間差異顯著新欧美老妇三级经典在线观看；不管是剪根苗還是帶根苗国产露出网站，木質(zhì)部和韌皮部含菌量之間的差異都非常顯著；上中下與木韌交互作用中日韩欧美午夜性爱福利，含菌量差異非常顯著欧美一级视频网站；最后篩選出來的強弱2個菌株HY3和93B，它們的纖維素酶活性分別為：1.955ug/和1.288ug/91在线精品网址，具有顯著的差別国产91九色视频；果膠酶的活性分別為：1.325 ug/和1.24ug/，沒有顯著的差別国产性交A级视频，而且HY3和93B兩個菌株細胞分泌的胞外多糖的含量差異很顯著久久r久久，分別為：7.08×10-8ug/cell和5.17×10-8ug/cell。

Methods: We collected 121 of clinical isolates of Enterobacter, including 87 isolates Enterobacter cloacae, 34 isolates Enterobacter aerogenes, all of the isolates were detected by cefoxitin three-dimension test, drug resistance phenotypes estimation, cloxacillin disc synergy test and cloxacillin-potentiated disc diffusion test respectively to find high-level AmpC β-lactamase isolates, drug resistance phenotypes estimation were also used to detected high induction producing AmpC β-lactamases isolates, meanwhile, we took three-dimension test as standard method, and compared to other methods, then calculated the coincidence rates, sensitivities and specificities of them.

采用頭孢西丁三維實驗国产AV。、紙片耐藥表型推斷法影音先锋每日最新AV资源久久、鄰氯西林紙片協(xié)同法、雙紙片鄰氯西林增效試驗分別檢測高產(chǎn)AmpCβ-內(nèi)酰胺酶株精品久久亚洲一区二区欧美，獲得高產(chǎn)AmpC酶在腸桿菌屬細菌中的表達狀況www.自拍偷拍.com。采用紙片耐藥表型推斷法檢測誘導(dǎo)和高產(chǎn)AmpC酶，同時以頭孢西丁三維實驗為標(biāo)準(zhǔn)jiujiuse在线，其他三種方法與之相比較a4yy成年私人影院在线看，分析它們檢測高產(chǎn)AmpC酶的符合率、靈敏度和特異性国产99精品免费观看老女人。

According to the ability of the field isolates of Gibberella zeae to grow on the PSA with varying carbendazim concentrations, three sensitivity levels of isolates were determined in vitro. The sensitive isolates could grow at 0.5 μg/ml, but could not grow at 100 μg/ml. The high resistant isloates could grow faster than R at 50 μg/ml, and also could grow at 100 μg/ml.No low resistant isolates, that could grow fast at 1.4 μg/ml but could not grow at 50 μg/ml, were found among the field isolates.

根據(jù)在0.5日韩综合小视频、1.4、5018禁肉肉无遮挡无码网站公司、100 μg/ml等不同濃度的含藥PSA平板上能否生長爽爽爽午夜福利试看，將玉蜀黍赤霉田間菌株對多菌靈的敏感性劃分為：敏感、中抗和高抗等3個水平5060av，其中S菌株在0.5 μg/ml濃度下能生長色哟呦在线播放，但在≥1.4 μg/ml濃度下生長受到完全抑制；R菌株在1.4 μg/ml濃度下能快速生長，在50 μg/ml濃度下能緩慢生長网站观看jizz，但在≥100 μg/ml濃度下不能生長男人的天堂在线播放；HR在≥100 μg/ml濃度下仍能生長。

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

中文題名傳染性支氣管炎致腺胃病變毒株變異的分子基礎(chǔ)及我國和東南亞分離株的分子流行病學(xué)研究副題名外文題名 Molecular basis for variation of proventriculus pathogenic IBV isolates and molecular epidemiology of IBV isolates from China and southeast Asia 論文作者蔣貽海導(dǎo)師陳溥言教授學(xué)科專業(yè)預(yù)防獸醫(yī)學(xué)研究領(lǐng)域\研究方向動物分子病毒學(xué)及免疫學(xué)學(xué)位級別博士學(xué)位授予單位南京農(nóng)業(yè)大學(xué)學(xué)位授予日期2002 論文頁碼總數(shù)127頁關(guān)鍵詞雞傳染性支氣管炎傳染性支氣管炎病毒腺胃毒株館藏號BSLW /2003 /S855 /9 從我國接種過傳染性支氣管炎疫苗的幼雞群的腺胃組織中分離得到三個傳染性支氣管炎毒株Q1欧美业余性爱、J2和T3欧美黄片在线播放，并對它們的部分生物學(xué)特性進行研究。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究不卡黄色网，於宜蘭五結(jié)垃圾場和臺中市環(huán)保局廚余堆肥樣品中共分離出 23 株具脂肪酶活性菌株6080亚洲三片级， 23 株菌株中經(jīng)過三油酸甘油酯的洋菜培養(yǎng)基於 50℃培養(yǎng) 5 天后，發(fā)現(xiàn)有 2 株菌的脂肪酶活性較強18岁禁网，分別為分離株 B61 和 M63欧美亚洲日韩国产一区；以本實驗室先前自臺大女九餐廳蔬果廚余堆肥中所篩選出的耐高溫脂解菌 Brevibacillus borstelensis SH168作為本實驗對照菌株，將分離株 B61色色欲望综合网、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液態(tài)培養(yǎng)基培養(yǎng)并測定其脂肪酶活性老人把我添到了高潮A片，發(fā)現(xiàn)分離菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，認為分離株 B61亚洲图片澳洲图片在线看、M63 適於產(chǎn)生脂肪酶的菌株福利a搞逼，分離株 B61 在 pH 5~9 皆可生長，生長溫度介於 20 oC ~ 60oC黄视频毛片网站；分離株 M63 在 pH 6~10 可生長亚洲无码视频四区，生長溫度介於 30 oC ~ 50oC； 2 株分離菌株以 16S rRNA定序結(jié)果分離株 B61 為 Bacillus circulans国产精品黄色网站大全，分離株 M63 為 Bacillus 屬日本精品乱伦一区二区三区视频。

Of 549 isolates, 20 isolates (about 3.6% of the total isolates) showed significant activity against P. ultimum and 15 isolates (about 2.8% of the total isolates) against R.

結(jié)果表明，對終極腐霉亚洲欧美高清综合不卡一区、立枯絲核菌的半抑制稀釋倍數(shù)（ID50）在10以上的活性菌株分別為20株（占總菌株的3.6%）和15株（占總菌株的2.8%）国产一级A片爽视频。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2組曲霉單克隆抗體建立特異性識別不同種類曲霉抗原的檢測方法。方法采用天然煙曲霉抗原免疫www日韩一区电影网，獲得廣譜針對曲霉抗原的單克隆抗體亚洲综合专区；采用重組煙曲霉抗原獲得特異性針對煙曲霉抗原的單克隆抗體，用間接免疫熒光鑒定91国自产精品中文字幕亚洲，并分別建立2種雙抗體夾心elisa法无码天堂一区二区三区，對19種常見的環(huán)境和臨床分離曲霉株、馬爾尼菲氏青霉菌及念珠菌培養(yǎng)液進行檢測美女被cao视频在线观看免费。結(jié)果間接免疫熒光顯示whsanxi.com，用天然煙曲霉抗原免疫獲得的單克隆抗體(mabs-1)可廣譜識別多種曲霉分離株，而重組煙曲霉抗原獲得的單克降抗體(mabs-2)僅能特異性結(jié)合臨床和環(huán)境分離的煙曲霉抗原中文字字幕在线中文第6页。用mabs-1建立的雙抗體夾心elisa法可檢測19種常見曲霉株培養(yǎng)液日韩免费A∨片免费网站；用特異性針對煙曲霉抗原單克降抗體(mabs-2)建立的雙抗體夾心elisa法可特異性檢測臨床和環(huán)境分離株煙曲霉培養(yǎng)液在线视频se；與其他曲霉株無交叉反應(yīng)；2種雙抗體夾心elisa法與馬爾尼菲氏青霉菌及念珠菌培養(yǎng)液均無交叉反應(yīng)欧美区一区二区。