①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

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The number of RGCs in ONID group retrogradely labeled by FluoroGold is more than that in non-ONID group.

視神經(jīng)超微結(jié)構(gòu)顯示日韩在线的第一页，ONID組髓鞘和軸突變性的程度明顯小于對(duì)照組。

The regenerated RGCs were labeled retrogradely by granular blue, and the changes in number of regenerating RGCs in each retina were observed under fluorescent microscope.

用粒藍(lán)逆行標(biāo)記再生的 RGCs好男人社区WWW资源，在熒光鏡下觀察視網(wǎng)膜平鋪片再生 RGCs的數(shù)量變化久久精品免费少妇高潮特黄。

The proportion of the number of double-labeled neurons with NOS/TMR was 55％(17/31)and 34％(17/49) in the whole population of the NOS positive neurons and retrogradely neurons,respectively.

計(jì)數(shù)顯不：NOS/TMR雙標(biāo)記細(xì)胞分別占NOS陽(yáng)性細(xì)胞總數(shù)的55％(17/31)和TMR逆行標(biāo)記細(xì)胞總數(shù)的34％(17/49)。

The regenerated RGCs were labeled retrogradely with FluoroGold,and the size of regenerating RGCs body in each retina and the shape of axon in ON sections were observed under fluorescent microscope.

用熒光金逆行標(biāo)記再生的軸突涩涩视频一区二区三区，在熒光鏡下觀察視網(wǎng)膜平鋪片中再生的視網(wǎng)膜節(jié)細(xì)胞大小及視神經(jīng)切片內(nèi)再生的軸突免费视频在线观看10000网站视频。

RESULTS: After injection of TMR into the PBN,the retrogradely labeled neurons,mostly small and medium-sized(below20 μm),were found in the INV ipsilateral to the injection side.

結(jié)果：逆行標(biāo)記細(xì)胞主要位于注射側(cè)的INV，大多屬20μm以下的中欧美视频在线、小型細(xì)胞国产+黄视频。

RESULTS: In the rats injected with FG into the PBN, many neurons retrogradely labeled with FG were observed in the ventrolateral subdivision at the caudal level of the PAG and in the DR.

結(jié)果： 將FG注入PBN后，在PAG的尾段腹外側(cè)區(qū)和DR觀察到許多FG逆標(biāo)神經(jīng)元美女免费视频一区；5人人鲁？？羞羞呦呦视频在线免费播放？人人操人人摸在线？

After the animals were killed, retrogradely labeled surviving RGCs were counted in the flat-mounted retinas and the mean RGC density was estimated.

于相應(yīng)存活時(shí)間點(diǎn)處死動(dòng)物久久久久亚洲AV无码专区电影，取出各組大鼠左側(cè)視網(wǎng)膜平鋪后計(jì)數(shù)存活RGC并得出RGC的平均密度。

After 12weeks, RGCs were retrogradely labeled with fluorogold and RGC density was analyzed.

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Neurons innerating the L5/6 interertebral discs, retrogradely labeled with fluoro-gold, were distributed throughout dorsal root ganglions from L1 to L6 in both groups.

結(jié)果台湾美女一级A片。兩組逆行熒光金示蹤顯示，支配L5/6椎間盤(pán)的神經(jīng)元分布在從L1到L6的DRG中国产小BBWBBWBBWvideos。