Starch esterification was proceeded with native cassava starch and three saturated free fatty acids: capric acid (C10:0), myristic acid (C14:0) and stearic acid (C18:0). The fatty acid modified starch was prepared by using lipase PS as the catalyst.

中文摘要本研究以天然樹(shù)薯淀粉為反應(yīng)基質(zhì)男人的天堂网最新网址，與三種不同鏈長(zhǎng)飽和脂肪酸羊蠟酸(C10：0又大又长的黄色视频、肉豆蔻酸(C14：0)與硬脂酸(C18：0)分別於水相及有機(jī)相行酯化反應(yīng)91自拍偷拍论坛欧美性爱，并且以工業(yè)中常用的脂肪水解酶lipase PS催化酯鍵合成，可得脂肪酸修飾淀粉(fatty acid modified starchwww.18岁禁止网页， FAMS)亚洲视频小说之无码视频。

It was observed after 24hrs of reaction that this lipase could compete very well with lipase from Candida antartica (93. 0％ for isoamyl butyrate synthesis and 95. 0％ for ethyl caproate synthesis) and lipase from Mucor miehei (75. 0％ for isoamyl butyrate synthesis and 93. 64％ for ethyl caproate synthesis).

反應(yīng)24h后，來(lái)自Candida antartica脂肪酶合成丁酸異戊酯和己酸乙酯的轉(zhuǎn)化率分別為93.0％和95.0％aaa视频色，來(lái)自Mucor miehei脂肪酶合成丁酸異戊酯和己酸乙酯的轉(zhuǎn)化率分別為75.0％和93.64％97热在线视频免费播放器。

The different factors involved in the process of the folding and activation of the microbial lipase are reviewed in this article,including lipase-specific foldase,lipase activation factor,prosequence,calcium ion and disulphide bond.

對(duì)微生物脂肪酶活性構(gòu)像的形成和激活機(jī)制進(jìn)行深入研究，有助于我們更好地了解脂肪酶的結(jié)構(gòu)和功能久久久久A级毛片免费看，為脂肪酶基因的大量表達(dá)和體外定向進(jìn)化奠定基礎(chǔ)黄色a片视频中文字幕在线观看一级。本文主要綜述了作為脂肪酶結(jié)構(gòu)成分的多種因子在脂肪酶活性構(gòu)像形成、激活及穩(wěn)定中的作用www.xxxx.xom。具體催化反應(yīng)體

This finding demonstrates that the regional codon optimization of the lip2 gene fragment at the 5'end can significantly enhance the expression level of recombinant Candida rugosa lipase 2 in the Pichia pastoris system.

這個(gè)發(fā)現(xiàn)同時(shí)也證實(shí)了本研究所采用的靠近5'端的局部密碼最優(yōu)化lip2基因片段最新91地址永久入口，對(duì)於基因重組 Candida rugosa lipase 2 在Pichia pastoris 系統(tǒng)中的蛋白質(zhì)表現(xiàn)量具有明顯的提升效果。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

與野生型相比日本免费的一级AAA片，突變體脂肪酶的酶活很低h动漫视频，不能在橄欖油平板上形成透明圈www.屌，但可以在以三丁酸甘油酯為底物的平板上形成透明圈看黄色录像一级片国产的。，表明第227位絲氨酸可能對(duì)脂肪酶酶酶活具有重要影響。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究免费一级操逼毛片，於宜蘭五結(jié)垃圾場(chǎng)和臺(tái)中市環(huán)保局廚余堆肥樣品中共分離出 23 株具脂肪酶活性菌株国产精品影院在线， 23 株菌株中經(jīng)過(guò)三油酸甘油酯的洋菜培養(yǎng)基於 50℃培養(yǎng) 5 天后，發(fā)現(xiàn)有 2 株菌的脂肪酶活性較強(qiáng)A片黄色视频免费，分別為分離株 B61 和 M63久久免费五月黄片；以本實(shí)驗(yàn)室先前自臺(tái)大女九餐廳蔬果廚余堆肥中所篩選出的耐高溫脂解菌 Brevibacillus borstelensis SH168作為本實(shí)驗(yàn)對(duì)照菌株，將分離株 B61不卡免费A片观看在线播放、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液態(tài)培養(yǎng)基培養(yǎng)并測(cè)定其脂肪酶活性有A片网站吗?，發(fā)現(xiàn)分離菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，認(rèn)為分離株 B61禁止理伦18、M63 適於產(chǎn)生脂肪酶的菌株成人精品视频在线日韩免费观看网址，分離株 B61 在 pH 5~9 皆可生長(zhǎng)，生長(zhǎng)溫度介於 20 oC ~ 60oC欧美一区黄片视频；分離株 M63 在 pH 6~10 可生長(zhǎng)国产又粗又猛又爽在线观看，生長(zhǎng)溫度介於 30 oC ~ 50oC； 2 株分離菌株以 16S rRNA定序結(jié)果分離株 B61 為 Bacillus circulans日韩国产欧美精品，分離株 M63 為 Bacillus 屬精品福利在线视频。

The thesis mentioned that the conformation of lipase molecular can be induced by substrate to be an activated one in organic phase through constructing a water-in-oil micro-emulsion system. The activated conformation was recorded using high cross linking degree polymer. The effect of inducement on the activity of recorded lipase polymer was studied, the catalyzed property of recorded lipase polymer in water phase was investigated, and the fluorescence spectrum of lipase solution and recorded lipase polymer under different kinds of denaturalized circumstance were analyzed.

本文提出通過(guò)構(gòu)建油包水型微乳體系，使酶分子能夠在有機(jī)相中被反應(yīng)底物誘導(dǎo)出活性構(gòu)象精品日韩手机版视频在线观看，利用高交聯(lián)度聚合物對(duì)該構(gòu)象進(jìn)行刻錄天天日天天色天天干，比較了底物誘導(dǎo)對(duì)刻錄酶活性的影響人人揉人人操人人爽，考察了刻錄酶在水相中催化特性，并對(duì)脂肪酶溶液和刻錄酶聚合物在各種變性條件下的熒光光譜進(jìn)行分析久久这里只有欧美精品。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本實(shí)驗(yàn)利用Prep Cell純化玉米萌芽谷粒和大豆子葉中的beta-淀粉酶狼人av在线，分別將兩種不同物種的beta-淀粉酶與胰脂解酶預(yù)先反應(yīng)后，再加入脂解酶反應(yīng)液中觀察活性的變化迷晕灌醉在线播放精品，結(jié)果發(fā)現(xiàn)脂解酶的活性未如預(yù)期www.xxxxx欧美，沒(méi)有抑制的效果產(chǎn)生，將甘薯beta-淀粉酶以同樣方法與胰脂解酶作用亚洲日韩深夜福利第一页，結(jié)果同上述兩種beta淀粉酶依舊沒(méi)有抑制的現(xiàn)象欧美在线激情丁香。

Lipase is one of the most widely used enzymes in industries such as leather, food and bio-diesel production.

脂酶(Lipase， EC3.1.1.3)是普遍應(yīng)用于皮革色呦呦观看、飼料及生物柴油工業(yè)的工業(yè)酶制劑人人摸人人做人人操，具有廣泛的應(yīng)用價(jià)值。

Novozym 435 was used for the synthesis of vitamin A plamitate. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid, at 30℃, in 10 mL hexane, containing 0% of water, with 10% of lipase(mass ratio, immobilized lipase to vitamin A plamitate). In these conditions, 75% of vitamin A acetate was converted into vitamin A plamitate. The immobilized lipase was reused about 6 batches.

對(duì)催化合成維生素A棕櫚酸酯反應(yīng)的脂肪酶和反應(yīng)介質(zhì)比較欧美性爱a片4色，同時(shí)對(duì)影響合成維生素A棕櫚酸酯反應(yīng)的因素（溫度国产欧美日韩综合视频在线、初始水含量、底物摩爾比日本高清不卡网址、反應(yīng)時(shí)間和酶量等）進(jìn)行了探討迷奸视频456，優(yōu)化了反應(yīng)條件：在10 mL不含水分的正己烷中，0.200 g 維生素A醋酸酯和0.468 g棕櫚酸在酶量為10％（指固定化酶與維生素A醋酸酯的質(zhì)量比）的固定化脂肪酶催化下黄色丝袜网站，在 30℃超碰97青青久久人人澡、190 r/min下反應(yīng)6 h，轉(zhuǎn)化率可以達(dá)到75%黄色在线观看www，固定化酶可連續(xù)使用6次以上婷婷五月综合色在线看。