The preparation process of equisetum pratense total alkaloid includes the following steps: coarse pulverizing equisetum pratense to obtain rough powder, methyl alcohol percolation to obtain percolate, recovering methyl alcohol, concentrating, degreasing by means of petroleum ether, alkalifying concentrate, CHCl3 extracting, soda lye washing, neutral water washing, recovering CHCl3 to obtain rough alkaloid, adsorption by using silica gel column, eluting, recovering solvent so as to obtain refined EPR.

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Formalin A mixture of about 40% formaldehyde, 8% methyl alcohol, and 52% water (the methyl alcohol is present to prevent polymerization of the formaldehyde). It is a powerful reducing agent and is used as a disinfectant, germicide, and fungicide and also as a general preserving solution.

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The results showed that the optimum conditions were as follows: methyl alcohol as the solvent, the ratio of oil to methyl alcohol 1:2.0, extraction temperature 32℃, extracting 4 times for once 10min.

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However, it is to be noted that activity of sodium hydroxide with respect to the ester exchange reaction is not sufficient, and in order to improve the conversion rate of the waste edible oil to 90%, it is necessary to add methyl alcohol (stoichiometrically required amount of methyl alcohol is about 3 mole with respect to 1 mole of vegetable oil) and catalyst excessively.

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Goal compound synthesis namely: Take the vanillic acid as outset raw material, obtains 4- hydroxyl - 3- anisole methyl formate with the methyl alcohol reflux conditions, then after the etherification, the nitration, the return to original state, the ring closure response obtains 6- methoxy - 7- animal pen oxygen radical kui zuo lin - 4- alkone, then passes through the chlorination, the substitution aniline, to escape responses again and so on animal pen oxygen radical, etherification to obtain the goal compound; The goal compound and the diethylamine had the amine substitution reaction to obtain TM1, namely 4- benzene amino - 6- methoxy - 7- [2- hydroxyl - 3-(N, N- two ethyl aminos) third oxygen radical] kui zuo lin; Through zuo has the etherification with the Austria niter to respond obtains TM2, namely 4- benzene amino - 6- methoxy - 7- [2- hydroxyl - 3-(2- methyl - 5- nitryl imidazole) third oxygen radical] kui zuo lin.

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Some research and analysis on the reaction of 2,4-difluoronitrobenzene and nucleophilic reagent alcohol sodium (methyl alcohol ,ethyl alcohol, propyl alcohol, n-butyl alcohol, t-butyl alcohol, et hl) and the reaction choice of opposite -F in different solvent and conditions have done in this paper.

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The production method as defined in claim 7, wherein the alcohol is selected from the group consisting of methyl alcohol, ethyl alcohol, isobutyl alcohol and mixtures thereof.

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A direct method to prepare nanometre catalyst of positive electrode for methyl alcohol fuel battery is to add strong oxidant to a mixed solution of manganese nitrate and some other metal nitrate, to coat them on the graphite electrode to have catalyst precursor produced, then to prepare the non-platinum nanometre catalyst of metal ion-doped manganese dioxide with carbon as a carrier by thermolytic reaction of the in-situ precursor. The prepared catalyst has the advantages of high electrical catalytic activity of methyl alcohol, high performance to protect against CO poison and low price, and it can lower the battery manufacturing cost effectively as well as can raise the entire electrical property of the battery so that the price performance and the market competitive edge of the battery are enchnaced effectively.

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At last, the effective approach was found that the 024-antibiotic can be purified by freez-dry, methyl alcohol soaking, acetone, chloroform, and methyl alcohol continuously washing, alumina and silica gel column chromatography.

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The methyl alcohol crude extract and phalloidin had theobvious inhibitory action on on H_(22) mice, the inhibition rate of the methyl alcohol crude extract andphalloidin were 43.48% and 65.15%.

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