Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球為供體克隆小鼠的研究該研究以C57BL/6 小鼠致密桑椹胚卵裂球為供體、昆明白小鼠MⅡ期卵母細胞為受體精品久久久久久无码中文版，研究了不同蔗糖濃度對核移植操作時間及重構(gòu)胚體外發(fā)育的影響狼友无码。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

結(jié)果：與無上皮細胞相比亚洲精品综合福利，共培養(yǎng)明顯促進了小鼠桑椹胚率（71.3%和61.2%，p.05）亚洲欧洲久久精品久久，囊胚率（50.0%和39.7%亚洲中文字幕5，p.05），囊胚孵化率（34.8%和24.3%999久久国产视频，p.05）和胚胎總細胞數(shù)（44.1和35.691精品在线国产，p.05）。結(jié)論：子宮內(nèi)膜上皮細胞能夠促進小鼠胚胎的體外發(fā)育国产拍拍上线视频，對早期胚胎的體外發(fā)育環(huán)境具有優(yōu)化作用。

Partial or complete removal of cumulus before fertilization had no effect on fertilization rates of oocytes, but complete removal of cumulus impaired embryo development, leading to reduced morula/blastula rates.

受精前去掉山羊卵丘或者去掉部分卵丘對受精率沒有顯著影響成年人视频在线观看免费品爱网，但是受精前完全去掉卵丘對于胚胎的后續(xù)發(fā)育有明顯影響国产美熟女乱又伦免费视频。

The morula within oocyst was light green, which was clustered by 3-8 granules, the wall was transparent in saline wet mounts.

生理鹽水涂片其囊壁透明，內(nèi)由 3～ 8個折光顆粒簇聚排列成淡綠色桑椹胚

Maternal TGFαmRNA was gradually reduced from oocyte to 8-cell stage and maintaining lower TGFαmRNA level was to morula stage, assumably TGFαmRNA transcription was begun from 8-cell stage with the zygote genome activation.

EGF亚洲国产欧美日韩久久、TGFα和EGFR mRNA在生殖系統(tǒng)中的表達通過RT-PCR的方法檢測了EGFwww.黄在线观看、TGFα和EGFR mRNA在生殖系統(tǒng)中的表達。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

異種克隆大熊貓重構(gòu)胚是通過將大熊貓成纖維細胞移入日本大耳白兔去核卵母細胞透明帶下后經(jīng)電融合構(gòu)建的97人人妻人人爽人人操，可在體外發(fā)育至孵化囊胚階段6080新觉伦午夜视频免费，我們應(yīng)用一種簡便、快速的異種重構(gòu)胚的鑒定方法一级免费一区二区精品尻逼大片，即通過染色體數(shù)目和特征染色體的存在初步鑒定出大熊貓-兔異種重構(gòu)胚的核遺傳物質(zhì)來源于大熊貓供核體細胞（后經(jīng)昆明動物研究所通過微衛(wèi)星DNA分析驗證了該結(jié)果的可靠性）色色色色色色色资源。