Objective To investigate the effect of different splitting solutions and decompaction treatment on the bisection of mouse embryos at morulae and blastocysts stage and their subsequent transfer after cultured.

目的 研究不同分割液和分割前胚胎去致密化與否對昆明白系小鼠的桑椹胚和囊胚的徒手分割以及分割后胚胎移植的影響。

By statistic analysis, there were significant differences with the average cell number of each morulae, early blastula and blastula between in vivo and in vitro, there were no differences with the hatching of blastula between in vivo and in vitro.

經(jīng)統(tǒng)計分析體內(nèi)桑椹胚私人福利影院、早期囊胚美女黄网在线视频免费观看、囊胚的每一胚胎平均細(xì)胞數(shù)與體外桑椹胚、早期囊胚欧美激性、囊胚的每一胚胎平均細(xì)胞數(shù)有顯著差異国语对白偷拍偷窥；擴張囊胚的體內(nèi)，體外每一胚胎平均細(xì)胞數(shù)沒有顯著差異美女被操出水高潮网站。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球為供體克隆小鼠的研究該研究以C57BL/6 小鼠致密桑椹胚卵裂球為供體免费A片视频日本一区二区三区、昆明白小鼠MⅡ期卵母細(xì)胞為受體，研究了不同蔗糖濃度對核移植操作時間及重構(gòu)胚體外發(fā)育的影響国内偷拍视频一区。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB欧美日韩国产精品，可明顯提高雙原核的形成率（38.9％vs 15.1％，P＜0.05）和桑椹胚/囊胚發(fā)育率（40.7％vs 26.8％在线看的a片，P＜0.05），但對其激活分裂率和總原核形成率無明顯影響（P＞0.05）亚洲一区二区三区久久精品视频。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

總之偷拍亚洲，我們的研究表明，DET玻璃液冷凍方法是簡單黄色视频在线免费观看黄黄网站、經(jīng)濟而又高效的欧美一级婬片欧美人妻，冷凍小鼠各階段胚胎都能獲得較高的存活率，其中受精卵的EDT30二步法1分鐘平衡組(66％)午夜性爱视频在线、桑椹胚的EDT30二步法1分鐘平衡組(94％)和EDT40二步法0.5分鐘平衡組(94％)怡红院精品视频在线观看极品、囊胚的EDT30二步法1分鐘平衡組(91％)和EDT40一步法1分鐘平衡組(91％)冷凍效果最好。

Indirect immunofluorescence was used to observe the distribution of fibronectin in human oocyte and 2-cell to morulae embryos.

纖維粘連蛋白均勻分布在體外未受精人卵母細(xì)胞和受精后人胚胎早期發(fā)育的不同階段的卵裂球表面www.aigou1314.com，各時期的免疫熒光強度未見明顯差別一区二区看美女视频。。透明帶未見纖維粘連蛋白表達(dá)。

Rates of fertilization and morulae/blastocysts in oocytes with fully expanded cumulus were significantly higher than those in oocytes with poorly expanded cumulus.

擴展良好的山羊卵巢卵母細(xì)胞的受精率和胚胎發(fā)育能力顯著高于卵丘擴展差的卵母細(xì)胞。

With the increase of the 1254 dosages, in the further development process of 2-cell embryo, there still occurred the development relayed and degeneration, so that the development rate of morulae and blastocysts decreascd significantly.

在受精卵的進一步發(fā)育中久久久这里有精品视频，隨著公鼠染毒劑量的增加美女图片一级卡片，發(fā)育到桑椹胚和囊胚的比例顯著降低，發(fā)育延遲胚及發(fā)育中退化變性胚比率顯著升高日本三级网页。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

應(yīng)用免疫組織化學(xué)方法和圖像分析技術(shù)研究整合素β_3亚洲全部黄色网站、FN和ER、PR在輸卵管粘膜內(nèi)的表達(dá)和變化黑人永久免费刺激视频；采用間接免疫熒光技術(shù)對FN在人卵母細(xì)胞和早期胚胎內(nèi)的表達(dá)分布進行觀察黄色黄色黄色黄片黄片视频黄片视频黄片；光鏡觀察輸卵管粘膜上皮細(xì)胞的形態(tài)學(xué)變化。結(jié)果：1日韩二区网站。整合素β_3在人正常輸卵管粘膜上皮纖毛細(xì)胞的胞漿內(nèi)表達(dá)苍井空全集百度影音。

In the experiment, the FSH+LH combinations were adopted to superovulate KM mice to ascertain the proper phase to gain morulae and blastocysts, three ways were adopted to split the morulae and blastocysts, demi-embryos were cultured in D-PBS, mTCM199, CZB, mM16 added with FCS or MS of different concentration, and transplantation of the blastocysts derived from demi-embryos was performed.

本試驗以昆明白小鼠為實驗動物，試采用FSH和LH進行超排處理欧美人妻视频一区二区三区，確定了該處理程序下獲取桑椹胚日韩高清第一页、囊胚的恰當(dāng)時間，比較了三種方法對桑椹胚和囊胚分割的效果国产乱交视频在线，采用了添加不同濃度FCS疯狂做受XXXX特大、MS的4種培養(yǎng)基對分割胚進行體外培養(yǎng)，并進行了半胚移植亚欧美福利一区二区三区。