Toxicity tests showed dextran and trehalose solutions had no effects on in vitro development of morulae after exposed for 5-20 mm at 250C.

抗凍保護劑溶液對桑椹胚的毒性試驗證明三级爆乳黄片在线看，25℃下，胚胎在葡聚糖和海藻糖溶液中平衡5-20分鐘，對其體外發(fā)育無不良影響。

The aim of the present study was to isolate and culture ESCs and EGCs from goat embryos and fetuses of different development stage, and to compare some factors influencing the efficiency of establishing those stem cell lines by adopting the methods of mouse and human. The results obtained were as follows: 1. A total of 46 Guanzhong dairy goats were superovulated with a result of total 307 embryos including 39 embryos of 4-cell to 16-cell, 128 morulae, 89 blastocysts and 51 hatched blastocysts. In the study, we obtain 6.67 embryos from per goat.

本研究選用關中奶山羊胚胎和胎兒為試驗對象三级片无码在线视频，參照小鼠和人ESCs和EGCs建系的方法，系統(tǒng)比較了不同培養(yǎng)條件和傳代方法對分離培養(yǎng)山羊ESCs和EGCs的影響色综合热无码热国产，主要獲得以下結論： 1本試驗共超排處理了46只/次關中奶山羊av黄片毛片在线，共獲得胚胎307枚，其中4~16細胞胚胎39枚国产手机小视频看片，桑椹胚128枚玖玖资源网在线，囊胚89枚国产专区网站，孵化胚51枚欧美一级情欲视频，平均每只山羊獲得6.67枚胚胎。

The rate of morulae/blastocysts was (59.5±3.2)%(P.05). In conclusion, the sequence culture system (SOF and granulosa cell+NCSU-23 FCS and POEC) can improve the development of procine parthenogenetic embryos.

結果表明美女黄色福利视频，SOF培養(yǎng)基和顆粒細胞＋添加胎牛血清的NCSU-23和輸卵管上皮細胞的序貫培養(yǎng)系統(tǒng)能較好的促進胚胎的發(fā)育女同一区二区三区。

During the first 3 days of sequence culture, SOF and granulosa cell enhanced the development of porcine parthenogenetic embryos and the cleavage rate (P.05) and the number of beyond 4-cell embryos. During the following 3 days. NCSU-23 supplemented with FCS and POEC supported the embryo to morulae/blastocysts.

在序貫培養(yǎng)的前3d，SOF培養(yǎng)基和顆粒細胞對胚胎的發(fā)育有促進作用欧美日韩国产中文在线专区视频，分裂率(P.05)和突破4細胞阻滯的數(shù)目顯著增加免费性爱a片在线，在培養(yǎng)的后3d，添加胎牛血清的NCSU-23和輸卵管上皮細胞能支持較多胚胎發(fā)育到桑囊胚精品拍拍，桑囊胚的發(fā)育率為(59.5±3.2)%(P.05)AV网站大全免费。

When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.22%, the highest rate in all groups. After the morulae cultured from thawed embryos from the best straw group and OPS group were transferred, all groups have obtained offspring.

在25℃溫度條件下細管法和OPS二步法玻璃化冷凍后的小鼠2-細胞胚胎，經(jīng)培養(yǎng)發(fā)育至囊胚后欧美视频在线观看图片小说，用H33342染色欧美国产亚洲精品高清不卡，在熒光顯微鏡下觀察并進行細胞計數(shù)。

Confocal microscope detection experiments showed that, in morulae and blastocysts, with theincreasing concentration of BPA, embryo of cytochrome C release rate increased. This showedthat the cytochrome C involved in BPA-induced the embryos apoptosis process. This processis regulated by the mitochondrial pathway.

激光共聚焦顯微鏡檢測細胞色素C結果顯示：在桑椹胚期和囊胚期一级毛片出来，隨著BPA處理濃度的升高黄色AV免费网址，釋放細胞色素C的胚胎比率逐漸增加，說明細胞色素C參與了BPA誘導胚胎細胞凋亡的過程日本免费一级二级视频网站，這一過程是通過線粒體通路來調節(jié)的美妇福利视频。

Among the three kinds of activation methods, electrical activation was the best with (67.75±1.03)% oocytes cleavage rate and (13.52±2.05)% morulae rate. The best parameters for electrical activation was 130 V/mm and 80 μs.

電激活比乙醇激活、A23187激活有更高的卵裂率(67.75±1.03)%和桑椹胚率(13.52±2.05)%三级黄片三级黄片三级黄片三级，且差異顯著(P.05)日韩无码啪啪片，最佳激活參數(shù)為130V/mm、80μsigao999视频网为爱寻找刺激、一次脈沖激活久久精品微拍国产微。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本試驗以昆明小鼠1-細胞期胚胎為實驗對象，以CZB培養(yǎng)液為基礎液在不同發(fā)育階段添加外源性ROS可以免费看的黄色，培養(yǎng)至桑椹胚亚洲黄色网站1、早期囊胚亚洲国产一级理论片在线播放、囊胚、擴張囊胚階段黄色色色色色色色网站，分別觀察胚胎的胚細胞數(shù)色色爱视频、分裂指數(shù)的變化。

The study was conducted at 25℃ to cryopreserve the Australia Boer goat blastocysts and compacted morulae by one or two-step straw (Experiment 1) and OPS methods (Experiment 2) with vitrification solutions (EFS30, ESF40, EDFS30 and EDFS40) or by slow freezing (Experiment 3) with cryopretectant (1.5 mol/L EG).

在25℃室溫下国内大量愉拍人妻精品视频，采用細管法和OPS法在线视频国产激情，以不同濃度的EFS、EDFS為玻璃化冷凍液国产亚洲AAA片在线看，對澳洲波爾山羊致密桑椹胚和囊胚進行玻璃化冷凍保存五月欧美色图日本激情。同時利用1.5mol/L EG為抗凍保護劑對胚胎進行常規(guī)法冷凍保存。

Methods\ The 6 5mol/L glycerol and 6% BSA were used as the vitrification solution for the cryopreservation of morulae and blastocysts embryos of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse.

采用 6 5mol L的甘油作為冷凍保護劑欧美熟妇丰满肥白大屁股，采用二步法對CBA亚洲全黄无码一级、NOD、C57BL 6JA毛片好看免费视频、ICR及CD1小鼠 3 5d的胚胎進行玻璃化冷凍非洲亚干操射，并比較了不同品系小鼠胚胎的復蘇率及移植受孕率。