The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

結(jié)果表明波爾山羊卵母細(xì)胞和早期胚胎都是端粒酶陽性看个毛片；依據(jù)電泳條帶在凝膠成像系統(tǒng)下的光密度計算相對總產(chǎn)品產(chǎn)量定量比較發(fā)現(xiàn)日韩A片网站，卵母細(xì)胞最低為25.348，4-細(xì)胞為273.832欧美乱伦视频，至8-細(xì)胞又降低到56.11718岁以下禁止进入黄色网站，隨后快速升高，桑椹胚為251.111久热精品视频，囊胚端粒酶活性最高為519.46欧美成在人现视频。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個部分，第一部分對影響豬卵母細(xì)胞體外成熟和孤雌激活后胚胎分裂的幾個重要條件進(jìn)行了比較研究国产熟女高潮免费视频，確立了一種較好的培養(yǎng)方法：與顆粒細(xì)胞共培養(yǎng)国产自偷自偷免费一区，找到了一種適合豬卵母細(xì)胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸亚洲中文国内精品福，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%，國外報道的最高成熟率為(85.7±4.1)%无码aiai；第二部分對豬卵母細(xì)胞孤雌激活的化學(xué)方法進(jìn)行了研究在线观看黄色视频毛片，確立了化學(xué)激活豬卵母細(xì)胞的兩種最佳方法：1將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞用20μmol/Lionomycin作用30min，再將卵母細(xì)胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中女人丨8毛片一级毛片，卵裂率和桑囊胚發(fā)育率達(dá)到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞在200μM/L的Thimerosal中處理20min无码H肉在线观看免费一区，再與8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%昨天看到看看黄色网站；第三部分對孤雌激活胚胎的培養(yǎng)條件進(jìn)行了研究人人操人人摸人人看，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡單培養(yǎng)基中添加顆粒細(xì)胞進(jìn)行前3天的培養(yǎng)，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細(xì)胞進(jìn)行后期的培養(yǎng)A片免费网址在线观看麻豆，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%黄片v视频；第四部分研究了IGF-I

Expression of tert and hprt genes were detected simultaneous in all single-copy embryos developed at 4-C (65h-post hCG, 4/4), 8-C (75 h-post hCG, 4/4), morulae (93h-post hCG, 4/4) and blastocyst (118h-post hCG, 4/4) stages and the level of transcription increased.

結(jié)果表明，持家基因hprt在成熟卵母細(xì)胞受精前后国产欧美日韩一区二区，以及胚胎早期發(fā)育過程中均存在轉(zhuǎn)錄本好黄好色成人小视频网站。40%卵母細(xì)胞中存在的tert mRNA在受精后很快降解，檢測不到99久久无色码中文字幕；胚胎基因組在2-C早期開始轉(zhuǎn)錄tert mRNA久久精品人人大爽人人爽n，轉(zhuǎn)錄水平逐漸上升。

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h轉(zhuǎn)為正常培養(yǎng)24h国产很爽很舒服的视频，不影響卵母細(xì)胞的成熟和孤雌激活能力亚洲爱爱免费视频，并且CHX抑制后再成熟的卵母細(xì)胞經(jīng)孤雌激活發(fā)育到囊胚的比例與對照組卵母細(xì)胞相似。8国产成人影院在线观看、體外受精后看欧美一级片子视频，CHX抑制后再成熟卵母細(xì)胞的多精受精現(xiàn)象顯著增加，發(fā)育到桑椹胚/囊胚的比例顯著低于對照組欧美三及片。9日免费在线精品、CHX抑制過程中皮質(zhì)顆粒仍能發(fā)生遷移，但是CHX抑制會對皮質(zhì)顆粒的遷移造成不可恢復(fù)的損傷日本久久黄色放视频，使再成熟的卵母細(xì)胞內(nèi)皮質(zhì)顆粒不能完全遷移怕怕怕免费网站18以上看。10、CHX抑制對山羊卵母細(xì)胞α-微管和微絲都沒有影響黄频美女裸体，無論是抑制后處于生發(fā)泡期的卵母細(xì)胞色色色789，還是抑制后再成熟的卵母細(xì)胞，微管和微絲的分布都與對照卵母細(xì)胞相似黄色片-级片。

Methods C 57BL/6J strain female mice mated with KM male mice, after seeing plug 3.5 da ys the compacted morulae and blastocysts were collected and bisected microsurgic ally by using special knife, obtained half embryos were cultured and observed.

C57BL/6J雌性小鼠與KM雄性小鼠交配欧美性爱爆操，見栓3.5d采集小鼠晚期桑椹胚和早期囊胚老熟妇嗷嗷叫91九色，應(yīng)用顯微手術(shù)刀直接分割法進(jìn)行胚胎二等份分割99爱99，所得半胚體外培養(yǎng)欧美毛多,另类在线，觀察其發(fā)育情況。