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The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

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Therefore, in order to solve the ethical problems produced by technology application, break the confusion and bafflement of human minds, and protect the value and dignity of human life, we have to return to the root of bioethical thoughts to establish the fundamental perspective about the truth and life.

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In order to show the purposes for the creation of Dragonwings and theprocess from misunderstanding to mutual understanding, the possibilities of cultural fusion and cultural exchange, and human nature transcending cultural differences, I will discuss how the Chinese culture is rediscovered by a Chinese American and how cultural misunderstanding leads to an unbalanced world, why understanding and tolerance is a bridge to balance and harmony, and how cultural integration and fusion restore the world into a harmonious one.

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In a market economy, competitive environment, companies in order to remain invincible, and must be closely related to survival and development with customers, build good relations with existing customers in the resources based on the maintenance of good And the relationship between customers and the continued development of new customers, companies with high-quality products, quality service, establish a good reputation in the eyes of the customers a good image; and responsibility to customers, constant innovation and development Their own products to meet customer demand for new and regulate the enterprise customer resource management, enhance customer service quality.

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