The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立檢測(cè)hBLyS蛋白含量的ELISA方法選用2種ELISA方案對(duì)hBLyS蛋白含量的測(cè)定方法進(jìn)行研究国产youjizz，方案一用鏈霉親和素做間接包被男人的天堂综合网尹人网，方案二用生物素-親和素系統(tǒng)做終反應(yīng)放大；優(yōu)化選擇2種檢測(cè)方法的反應(yīng)條件黑人一级a毛片免费视频观看；方法一的敏感度較低：方法二在hBLyS蛋白濃度為3.2～400 ng/mL范圍內(nèi)videossex性暴力野外，標(biāo)準(zhǔn)曲線的線性關(guān)系良好；從而建立了一種可檢測(cè)hBLyS蛋白濃度為ng級(jí)水平的ELISA方法www.18黄色视频在线观看。

For initial photochemical conversion efficiency of chlorophyll fluorescence parameters, Day change rate curve presented "V" type: apparently decreased at noon, and rose in the afternoon. It appeared "Mirror" relation with the changes of light intensity.

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The research results are highly reproducible owing to the repeatable micro-model technique.(2) Quantifying the Kr-S-P curves in two-phase flow. The validity and adaptability of multi-phase curves are verified using micro-model testing bed tailored to adequate scale of pores. Results obtained from the P-S micro-model tests generally agree with the threshold values of displacement mechanism offered by researchers using geometric shapes. The hysteresis of .experimental Kr-S curves of numerous two-phase flows are depicted in terms of connectivity and humidification. The P-S and Kr-S curves of two-phase flow are well-derived in conjunction with micro-model test results and empirical van Genuchten model. For the curve expressing the relation between saturation and capillary pressure, experimental results of water-air flow system corresponds to the theoretical data.

2二相流體對(duì)微模型實(shí)驗(yàn)之Kr-S-P關(guān)系：包括以孔頸尺寸比例組成微模型板實(shí)驗(yàn)平臺(tái)去證實(shí)多相流流動(dòng)主要特性曲線正確性與適用性；驗(yàn)證水和空氣二相流之P-S微模型實(shí)驗(yàn)欧美性爱日逼，與前人以幾何形狀所推估出來的置換機(jī)制門檻值大致吻合A片日本视频网址；以連通性及潤(rùn)濕性等解釋多組二相流體對(duì)Kr-S曲線實(shí)驗(yàn)之遲滯現(xiàn)象；以微模型實(shí)驗(yàn)結(jié)果配合van Genuchten特性曲線經(jīng)驗(yàn)式互推估二相流體對(duì)之P-S與Kr-S 曲線99爱精品1，推估結(jié)果良好avpian；以比例原則推估多組二相流體於毛細(xì)壓力-飽和度曲線的結(jié)果，顯示在水和空氣二相流體對(duì)為參考流體對(duì)時(shí)狼友黄色视频，推估其他組流體對(duì)吻合度最佳日韩无码专区天美传媒。

In certain range,the bioenrichment of the mealworm larvae to Zinc in reference to the dosage added in the fodder is accorded with cubic curve relation:Y=181.2517+20.9314X-0.4099X2+ 0.0025X3. And the enrichment ability of T. molitor larvae in reference to the weight is also accorded with the cubic model:Y=35.8325+61.4155X-10.2980X2+0.4434X3. The enrichment of the larvae to Zinc can get to a high level in 2 to 3 days. This is anadvantage to numerous rearing of the mealworm. But we do not find a good rearing way to enhance the rate of bioenrichment to Zinc. And the further research about this is anticipated.

第二部分：采用鋅作為添加元素，考查黃粉蟲對(duì)微量元素的富集規(guī)律不卡一级片，結(jié)果表明：(1)黃粉蟲蟲體鋅含量在一定范圍內(nèi)隨鋅添加量增加而呈三次曲線關(guān)系瑟网站，符合三次曲線方程：Y=181.2517+20.9314X-0.4099X~2+0.0025X~3；(2)黃粉蟲幼蟲對(duì)鋅富集能力隨蟲體大小變化而變化欧美激情一区二区三区在线免费看，與蟲大小亦呈三次曲線關(guān)系欧美大片aaaaa级；Y=35.8325+61.4155X-10.2980X~2+0.4434X~3在线看中文字幕；(3)黃粉蟲蟲體鋅含量隨著鋅飼料添加而迅速上升，可在短期2～3天內(nèi)達(dá)到較高水平免费看羞羞漫画，對(duì)于以黃粉蟲為材料進(jìn)行富鋅保健食品生產(chǎn)有很大優(yōu)越性婷婷激情偷拍自慰；(4)本試驗(yàn)并未找到一種較好的鋅添加方法來提高鋅富集量，這方面的研究有待繼續(xù)男人天堂.com免费。

However,it is the dissipa-tive mechanism,i.e.the mechanism of energy localization that attracts one′sattention for the problem of shock initiation.Since the test result of shock initia-tion about fine-grain explosive charge does not support hot spot ignition model,the author of this dissertation advances that the mechanism of energy localiza-tion is coincident with phonon-fracton crossover of fractal network for ultra-fine-grained charge and,moreover,analyzes the relation between shock sensi-tivity and porosity,which is consistent with shock Hugoniot curve for porousmaterials.

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