Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

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The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

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Countless neutrophilic leucocytes were seen in 53 cases or 94.6%, plasma cells in 39 cases or 69.6%, reticulum cells in 34 cases or 60.7%, atypical epithelial cell in 39 cases or 69.6%, and multinucleated giant cells in 31 cases or 55.4% out of 56 cases of active phase ulcerative colitis.

在56例活動性潰瘍結腸炎中亚洲中文字幕区，無數(shù)的嗜中性白細胞在53例（94.6%）中可見亚洲手机小视频网站，漿細胞在39例（69.6%）中可見，網(wǎng)狀細胞在34例（60.7%）中可見日韩精品网站日韩在线观看免费视频，不規(guī)則上皮細胞在39例（69.6%）中可見国产视频自拍视频，多核巨噬細胞在31例（55.4%）中可見。

In contrast, in 50 cases with irritable colon in which biopsy showed normal colonic mucosa, no plasma cells or atypocal epithelial cells were seen, although reticulum cells and multinucleated giant cells were in only one case or 2.0% and countless neutrophilic leucocytes were seen in 6 cases or 12.0%.

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However, in 2 cases with intestinal tuberculosis, another chronic inflammatory disease of the large bowel, reticulum cells and countless neutrophilic leucocytes were seen in both cases , and plasma cells , atypical epithelial cells, and multinucleated giant cells in one case, In 7cases with amebic colitis countless neutrophilic leucocytes were seen in 2 cases (28.6%), plasma cells, reticulum cells, atypical epithelial cells, and multinucleated giant cells in one case (14.3%) respectively.

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However , countless neutrophilic leucocytes were seen in 3 cases(15.8%), plasma cells and reticulum cells in 2 cases (10.5%), and atypical epithelial cells in one case (5.3%) out of 19 cases in remission of ulcerative colitis .

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Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

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In 82 cases with cancer of the large bowel neutrophilic leucocytes were also seen in 20 cases or 24.4%, plasma cells in 5 cases or 6.1%,reticulum cells in 3 cases or 3.7%, and cancer cells in 77 cases or 93.9%, Therefore , the above cytologic fingdings are thought to be not pathognomonic to ulcerative colitis, but represent chronic inflammation of the large bowel.

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The injured gill cells had a different ultrastructure, with endoplasmic reticulum dilated and vesiculated, mitochondrial cristaes partially disintegrated, gill fibre cuticles denatured and dropped. The ultrastructural damages to hepatopancreatic cells were characterized by the decrease of microvilli, swelling and breaking up of mitochondria, expansion of endoplasmic reticulum, disruption and vacuolization of nucleus, tumefaction of nuclear membrane and increase of lipid droplets. As for the toxicological changes of myocardial cells, the mitochondria were swelling, having a lot of vacuolus and disintegrating, while the myofibrils were irregular with endoplasmic reticulum dissolved.

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