Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常規(guī)引物對(duì)感病植物的總RNA進(jìn)行RT-PCR擴(kuò)增直接观看黄的免费网站，獲得CMV RNA3全長(zhǎng)克隆xxxxx黄，經(jīng)測(cè)序顯示該RNA3序列屬于CMV亞組Ⅱ欧美在线中文字幕整片视频网站免费；通過(guò)在衛(wèi)星dsRNA兩端分別加上15nt的單鏈DNA接頭久久AV先锋影音资源网站，以接頭互補(bǔ)序列為引物進(jìn)行擴(kuò)增獲得一個(gè)383nt的衛(wèi)星RNA的全長(zhǎng)序列。

Finally a all-digital recorder system based on RAID5 which has a RT-Linux real-time software operation system and intermediate/video frequency acquisition card driver to overcome such problems as real-time quality reliability chunnel expansibility and standardization.

最終提出了以RT-Linux實(shí)時(shí)操作系統(tǒng)為軟件平臺(tái)和專(zhuān)門(mén)設(shè)計(jì)的中頻和視頻采集卡驅(qū)動(dòng)為底層硬件平臺(tái)的基于RAID5陣列的全數(shù)字記錄系統(tǒng)香蕉香蕉国产片一级一级毛片，全面解決了記錄實(shí)時(shí)性国产jiuse视频网站最新地址、可靠性以及通道擴(kuò)展性和標(biāo)準(zhǔn)化的技術(shù)難題。

Based on the theory of large eddy simulation and the method of VOF , the initial breakup process of fuel liquid jet, which eject from the nozzle exit at the time scale of micro-second level, is investigated. The mechanism of primary atomization is analyzed, and the initial breakup model of liquid jet is presented. By modeling and numerical simulation of atomization for a diesel nozzle under the given conditions, the initial umbrella-like spray shapes are obtained, which fits better with the experimental results. Meanwhile, the droplets secondary breakup process, such as leeward RT breakup, dumbbell-like breakup and droplets coalescence, etc. are exhibited visually.

基于大渦模擬LES理論和VOF方法，考察了燃油噴霧初始時(shí)刻即時(shí)間尺度為微秒級(jí)的液柱破碎過(guò)程在线日韩成人电视播放网站视频免费，分析了初始破碎的機(jī)理国产一级片操逼视频，給出了該時(shí)間尺度下液柱初始破碎過(guò)程的模型；通過(guò)對(duì)一特定條件下的柴油機(jī)噴油器的建模和噴霧過(guò)程的大渦數(shù)值模擬狼友视频入口首页面，獲得了液柱初始時(shí)刻"傘狀頭部"的噴霧形態(tài)97人妻无码视频在线一区，所得計(jì)算結(jié)果與相應(yīng)的試驗(yàn)數(shù)據(jù)符合較好；數(shù)值模擬還直觀地展現(xiàn)了液滴背風(fēng)RT破碎美国av毛片、啞鈴型破碎以及液滴的聚集融合等液滴的二次霧化過(guò)程亚洲校园欧美国产另类性色。

Methods The designed flow chamber had provided flow shear stress at 0.5, 1 Pa (5 and 10 dyn/cm^2) for a period of 1 h, 3 hand 6 h respectively. Semi-quantitive RT-PCR was used to detect the expression of megalin/cubilin mRNA in cultured renal tubular epithelial cells.

應(yīng)用流室系統(tǒng)提供0.5、1Pa(5日色视频精品、10 dyn/平方公分)的剪切應(yīng)力處理培養(yǎng)的腎小管上皮細(xì)胞日韩欧美性爱综合视频，分別作用1、391看片婬黄大片在线观看、6h后，用RT-PCR法檢測(cè)megalin/cubilin mRNA的表達(dá)欧美丝袜精品一区二区。

The protein and mRNA level of MCP-1 and IL-8 in U937 cells were determined by RT-PCR and ELISA.

用ELISA和RT-PCR方法測(cè)定MCP-1和IL-8的蛋白和mRNA水平性生活一级A片。

Results: A clear-cut endocardium image was obtained by both RT-3DE and CMRI in all but 1 volunteer.

RT-3DE是一種定量評(píng)價(jià)左心室容量-時(shí)間曲線(xiàn)的可行、準(zhǔn)確的新方法AV免费九九在线观看。

It was proved by RT-PCR that Pinb gene expressed itself in grain endosperm.

RT-PCR證實(shí)了Pinb基因在籽粒胚乳中的表達(dá)欧美一级黄色片99。

Expressions of eNOS and COX2 mRNA and proteins were assessed by immunohistochemical technique and RT-PCR.

用免疫組化和RT-PCR技術(shù)檢測(cè)肺組織eNOS和COX2 mRNA和蛋白表達(dá)。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR從C57BL/6J小鼠的十二指腸腸系膜黏膜組織中釣取腸激酶輕鏈的cDNA日韩欧美在线视频观看，將其克隆入pET32a原核表達(dá)載體中性a视频在线观看，并在大腸桿菌BL21(DE3)中進(jìn)行表達(dá)，然后以鎳親和層析法對(duì)表達(dá)產(chǎn)物進(jìn)行純化免费无码大黄网站在线观看。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

為克隆表達(dá)牛腸激酶輕鏈編碼基因曰韩欧美不卡片，以期應(yīng)用于融合蛋白的切割與純化，從市售北方黃牛十二指腸組織中提取總RNA玖玖在线资源www网，以RT-PCR方法擴(kuò)增其cDNA片段99热精品3，將此片段克隆于pUCmT載體中，經(jīng)過(guò)特異性限制性?xún)?nèi)切酶酶切分析片段正確后黄色网站aaaa，進(jìn)行全序列分析精品国产一区二区三区国产区。