In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines.

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In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT－PCR protocol and analysed by polyacrylamide gel electrophoresis.

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Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

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Methods The level of EGF mRNA in the cortex and medulla of porcine kidney was measured by RT-PCR technique.

采用RT-PCR技術(shù)分別檢測豬腎皮質(zhì)和髓質(zhì)中的EGF mRNA活性97亚洲熟妇自偷自拍另类图片。

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

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Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

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The method on trans fatty acids analysis in food is very important. The effects of methylate sodium, potassium hydroxide, sulfuric acid and boron trifluoride in methanol and their different concentration and dosage on TFA analysis were studied. The chromatographic condition and the influence of time and temperature on the esterifation of TFA using boron trifluoride in methanol were optimized. A method for analyzing TFA were established by GC based on boron trifluoride in methanol and RT-2560(100m×0.25mm×0.2μm) capillary column.

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RT-PCR was applied to detect changes of hepatic hepcidin gene expression on model different stage. Bone density was detected and decalcification bone microtome section and hepatic histological section were observed and analyzed on the 14th day. Results Compared with control group, hepatic hepcidin gene expression of test group on different stage had obvious differences.

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The results showed that the virus isolation and identification could generate considerably reliable results but the procedure was complicated and costed long time; microtomy fluorescent antibody detection was fast, but the specificity was low and needed to exclude the false positive from the results. Sandwich ELISA detection combined the merits of the former two methods, but it generated the false negative results when the samples were in bad preservation. Compared with the others, RT-PCR was fast, high sensitive and especially useful for detecting the CSFV directly from the samples. The specificity could be raised by using the nested-PCR to detect the CSFV from the simples, especially when the simples were in bad preservation.

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Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen.

方法利用RT-PCR結(jié)合RACE技術(shù)克隆短穗魚尾葵花粉中泛變應(yīng)原profilin的全長基因亞洲三級片性愛，并進行序列分析。