Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR結(jié)合RACE技術(shù)克隆短穗魚尾葵花粉中泛變應(yīng)原profilin的全長(zhǎng)基因黄页网站在线大全免费观看，并進(jìn)行序列分析wwww麻豆视频。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后設(shè)計(jì)帶有酶切位點(diǎn)的特異性引物，采用RT-PCR獲得整個(gè)短穗魚尾葵花粉profilin的開放閱讀框草草影院日本韩国欧美，將其與pET28a載體連接并轉(zhuǎn)化大腸桿菌BL21(DE3)進(jìn)行誘導(dǎo)表達(dá)久久AV草，通過(guò)Ni(上標(biāo) 2+)親和層析柱對(duì)重組蛋白進(jìn)行純化，采用Western-blot檢測(cè)其IgE結(jié)合活性免费看特大黄片视频。

The study in this paper, a Pollen-specific Lysine-rich Protein cDNA, from tomato by RT-PCR was obtained. A plant expression plasmid pBIUB-TSB was constructed and lysgene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter.

本實(shí)驗(yàn)通過(guò)RT-PCR從番茄花粉中獲得高效特異表達(dá)的高賴氨酸基因TSB的cDNA序列国产未成18禁止免费，構(gòu)建其受控于單子葉植物Ubil強(qiáng)啟動(dòng)子下的高效表達(dá)質(zhì)粒pBIUB-TSB。

Expression of peripheral monocyte MMP-9 was measured by RT-PCR and Western blot.

分離大鼠外周血單核細(xì)胞黄色理论在线观看，用RT-PCR和Western blot檢測(cè)MMP-9表達(dá)的變化挨操毛片。

The results of RT-PCR showed that the gene was expressed at all examined developmental stages and tissues of B. mori.

RT-PCR實(shí)驗(yàn)表明該基因在本實(shí)驗(yàn)所調(diào)查的家蠶不同發(fā)育時(shí)期和組織中都有表達(dá)。

The cDNA encoding AChE from susceptible and propoxur-resistant strains of Musca domestica had been cloned using RT-PCR.

2利用RT-PCR技術(shù)克隆了家蠅敏感和殘殺威抗性品系的乙酰膽堿酯酶基因日日射日日插日日干。

The cDNA sequence encoding ubiquitin from Musca domestica was cloned by RT-PCR and sequenced.

本研究根據(jù)GenBank已登錄的真核生物泛素編碼框的氨基酸序列91AV导航站，設(shè)計(jì)一對(duì)簡(jiǎn)并引物，RT-PCR克隆了家蠅Musca domestica泛素基因的編碼區(qū)cDNA序列永不卡的黄色网站，并進(jìn)行了測(cè)序亚洲最大人成在线视频。

TTC staining,RT-PCR and Western blot analysis were used to study the effects of muscone on infracted volume,EAAClmRNA and protein levels.

運(yùn)用Western blot法和TTC染色觀察缺血區(qū)EAAC1表達(dá)和梗塞體積；采用RT-PCR和Western blot法欧美性视屏，測(cè)定海馬EAAC1 mRNA和蛋白在缺血1h18禁黄黄污污网站在线看、6h、24h的變化女人的五夜天堂视频。

A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus S4 gene sequence. The specific RT-PCR product was success-fully obtained and cloned from the Muscovy duck reovirus ZJ99 strain.

參考已發(fā)表的番鴨呼腸孤病毒的S4序列設(shè)計(jì)了1對(duì)擴(kuò)增σC蛋白基因的引物欧洲免费黄片，對(duì)mDRV ZJ99株的RNA抽提物進(jìn)行RT-PCR擴(kuò)增明星换脸国产欧美一区，獲得了特異性的擴(kuò)增片段亚洲视频在线不卡。

Methods:The dynamic cytotoxicity of ADR or / and NaBu was evaluated by MTT assay. The telomerase activity was analyzed by TRAP and silver staining. The expression of hTERT was assessed by RT-PCR.

MTT法檢測(cè)NaBu、ADR以及兩藥聯(lián)用的藥效動(dòng)力學(xué)特征曰韩美女裸体视频A片久久；TRAP-銀染法檢測(cè)端粒酶活性婷婷激情五月综合丁香社区；RT-PCR法檢測(cè)hTERT mRNA表達(dá)很黄很污的软件。