Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根據(jù)H5亚洲三级片中文字幕、H9亞型HA基因序列欧美男女真空尺度在线，分別設(shè)計(jì)一套特異性引物免费看一级人妻片，其上综合视频一区二区、下游引物分別位於裂解位點(diǎn)兩側(cè)亚州+日韩+国产+无码，應(yīng)用RT-PCR一步法檢測AIV H5和H9亞型国产精品爱V在线观看，并驗(yàn)證所建立方法的敏感性；通過檢測NDV、IBV和IBDV等RNA病毒及電腦軟件模擬檢測激情国产黄色视频，驗(yàn)證其特異性国产高清在线视频一区二区。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法檢測免疫血清抗體對ED的親合力。2在线观看成人短视频网站、對DNA疫苗組小鼠制備OVA致敏哮喘模型男人的天堂综合网，檢測氣道壓力峰值—時(shí)間指數(shù)、支氣管／肺泡灌洗液中炎性細(xì)胞數(shù)量日韩3级电影网，PAS特染觀察小支氣管杯狀細(xì)胞數(shù)量及RT-PCR法檢測肺組織中粘蛋白MUC5AC mRNA水平国产视频33区，免疫組化和RT-PCR法檢測GM-CSF的蛋白與mRNA水平。

A HIL simulation system based on concurrent computer is presented and the RT-Lab is applied to solve real-time control problem. Using the concurrent computer and RT-Lab, both anti-skid braking controller and hydraulic system can be put into the simulation loop.

提出了飛機(jī)剎車系統(tǒng)半實(shí)物仿真設(shè)計(jì)方案打开免费黄色网站在线观看，在并行計(jì)算機(jī)硬件平臺(tái)基礎(chǔ)上欧美成αⅴ人在线视频，將實(shí)物(如剎車控制單元、液壓模擬系統(tǒng)等)接入仿真回路日本人人干，在RT-LAB環(huán)境下構(gòu)建了仿真平臺(tái)黄片欧美性爱。

Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

為驗(yàn)證該設(shè)想，本研究建立了一步RT-PCR技術(shù)擴(kuò)增體系精品狼友视频在线播放，并利用該體系擴(kuò)增和分析了國內(nèi)幾種蔬菜作物部分CMV分離物的2b基因序列同源性亚洲国模私拍人体GOGO图片，選擇特定分離物的全長2b基因，先利用PVX侵染性載體初步分析其正義和反義序列間的互作人妻大白腚，再構(gòu)建植物表達(dá)載體轉(zhuǎn)化煙草和番茄A片免费看视频，并研究轉(zhuǎn)基因煙草抗CMV的表現(xiàn)，獲得了抗CMV的轉(zhuǎn)基因煙草植株国产淑女色视频。

Soybean ; Bean pod mottle virus ; one step real-time fluorescent RT-PCR ; one step RT-PCR ; detection

大豆国产一级a片精品久久；菜豆莢斑駁病毒；一步法實(shí)時(shí)熒光RT-PCR 15级黄片电影；一步法RT-PCR 欧美日韩另类自拍动漫；檢測

RT-3DE shows clearly full view of the primary atrial tumor than two-dimensional echocardiography and obvious image differences between atrial myxoma and malignant atrial tumor. It is a convenient, quick and precise modality for diagnosing primary atrial tumor and may be of significant clinical value for the diagnosis of primary atrial tumor.

RT-3DE比二維超聲心動(dòng)圖更清晰顯示原發(fā)心房肺瘤全貌形態(tài)，黏液瘤與惡性心房腫瘤圖像特征差別明顯老师洗澡让我吃奶羞羞漫画，RT-3DE是診斷原發(fā)心房腫瘤簡便色欲aⅤ、快捷和準(zhǔn)確的檢查方法，RT-3DE對原發(fā)心房腫瘤具有重要臨床診斷價(jià)值欧美永久视频免费。

Methods Semi-quantitative RT-PCR and real-time RT-PCR were applied to detect the expression level of PTX1 in 36 NPC and 8 chronic nasopharyngitis biopsies.

用RT-PCR和熒光定量RT-PCR檢測PTX1在36例鼻咽癌及8例慢性鼻咽炎中的表達(dá)www.玖玖爱免费视频。

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

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The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

應(yīng)用雜交瘤技術(shù)制備ZNRD1的首個(gè)單克隆抗體；2）利用RT-PCR亚洲激情小说、Western blot和免疫組化檢測ZNRD1在胃癌組織、胃炎組織欧美精品视频专区在线、正常胃上皮組織久久综合男人、胃癌細(xì)胞和正常胃組織上皮細(xì)胞中的表達(dá)；3）構(gòu)建ZNRD1的小干擾RNA載體在线黄色视频了,，并測序鑒定日本三级网；4）利用脂質(zhì)體將ZNRD1的真核表達(dá)載體及其空載體轉(zhuǎn)染胃癌細(xì)胞（AGS、SGC7901免費看男女高潮又猛又爽、MKN28）和小鼠成纖維細(xì)胞（NIH3T3）欧美成人版中文字幕，G418篩選后進(jìn)行鑒定；5）利用脂質(zhì)體將ZNRD1的小干擾RNA載體及其空載體轉(zhuǎn)染藥敏胃癌細(xì)胞（SGC7901）成年人一级黄色视频、正常胃組織上皮細(xì)胞（GES-1）九月色色色色色、對長春新堿耐藥的胃癌細(xì)胞（SGC7901/VCR）、藥敏白血病細(xì)胞（HL-60）欧美A片在线中文字幕、對長春新堿耐藥的白血病細(xì)胞（HL-60/VCR）猛男好爽好爽男人天堂，G418篩選后進(jìn)行鑒定；6）利用MTT實(shí)驗(yàn)檢測ZNRD1高/低表達(dá)對細(xì)胞（AGS一级毛片黄久久久免费看、SGC7901午夜三级免费视频、MKN28、NIH3T3国产精品日韩、GES-1）生長的影響jizz在线视频；7）通過軟瓊脂克隆形成實(shí)驗(yàn)檢測上調(diào)ZNRD1對AGS、MKN28細(xì)胞克隆形成能力的影響黄色视频观看免费的黄色网站；8）通過裸鼠成瘤實(shí)驗(yàn)檢測上調(diào)ZNRD1對AGS精品一级黄色、MKN28細(xì)胞體內(nèi)成瘤性的影響；9）通過流式細(xì)胞儀分析ZNRD1高/低表達(dá)對細(xì)胞（AGS欧美激情亚洲熟妇、MKN28免费福利黄视频、NIH3T3、GES-1）的細(xì)胞周期的影響葡京99热这里只有精品_；10）通過流式細(xì)胞儀分析上調(diào)ZNRD1對細(xì)胞（AGS一进一出免费视频、MKN28、NIH3T3）的凋亡的影響蝌蚪久久精品午夜；11）通過MTT實(shí)驗(yàn)檢測ZNRD1高/低表達(dá)對細(xì)胞（SGC7901色哟哟网站一区二区、SGC7901/VCR、HL-60、HL-60/VCR）體外藥物敏感性的影響色淫综合区；12）通過腎包膜下移植法檢測ZNRD1高/低表達(dá)對細(xì)胞（SGC7901欧美1区A片区、SGC7901/VCR）體內(nèi)藥物敏感性的影響；13）通過流式細(xì)胞儀分析ZNRD1高/低表達(dá)對細(xì)胞（SGC7901国产亚洲精品激情久久、SGC7901/VCR美女黄男的插女的、HL-60、HL-60/VCR）內(nèi)阿霉素蓄積和泵出的影響96无码国产在线播放；14）通過透射電鏡檢測上調(diào)ZNRD1對SGC7901細(xì)胞凋亡敏感性的影響强奸中字三区四区；15）通過流式細(xì)胞儀和DNA梯度試驗(yàn)檢測ZNRD1高/低表達(dá)對細(xì)胞（SGC7901、SGC7901/VCRⅴ黄片視頻、HL-60）凋亡敏感性的影響6080午夜三级；16）通過基因芯片檢測ZNRD1高/低表達(dá)對胃癌細(xì)胞內(nèi)基因表達(dá)譜的影響；17）利用RT-PCR自拍少妇亚洲、Western blot對基因芯片的結(jié)果進(jìn)行鑒定日本三级a网站；18）利用報(bào)告基因?qū)嶒?yàn)檢測ZNRD1對cyclin D1的啟動(dòng)子活性的調(diào)節(jié)作用；19）利用報(bào)告基因?qū)嶒?yàn)檢測ZNRD1高/低表達(dá)對MDR1的啟動(dòng)子活性的調(diào)節(jié)作用黄色视频海外免费；20）利用激酶試驗(yàn)檢測ZNRD1對cyclin E-CDK2 激酶活力的影響综合色亚洲自拍偷拍欧美；21）利用反義核酸技術(shù)抑制p21的表達(dá)；通過流式細(xì)胞儀檢測抑制p21對ZNRD1介導(dǎo)的細(xì)胞周期阻滯的影響在线日韩素人自拍网页观看免费视频；22）利用反義核酸技術(shù)抑制p27的表達(dá)一区二区高清无码网站；通過流式細(xì)胞儀檢測抑制p27對ZNRD1介導(dǎo)的細(xì)胞周期阻滯的影響；23）利用脂質(zhì)體轉(zhuǎn)染法上調(diào)Skp2的表達(dá)欧美性爱熟女视频；通過流式細(xì)胞儀檢測上調(diào)Skp2對ZNRD1介導(dǎo)的細(xì)胞周期阻滯的影響噜噜噜色；24）利用Western blot檢測ZNRD1對p27和Skp2的蛋白穩(wěn)定性的影響；25）利用微血管密度實(shí)驗(yàn)檢測ZNRD1對AGS中文字幕在线看、MKN28細(xì)胞裸鼠移植瘤微血管形成的影響91黄在线观看国产；26）利用ELISA檢測ZNRD1對AGS、MKN28細(xì)胞培養(yǎng)上清和移植瘤勻漿中VEGF165含量的影響玖玖在线播放网站；27）利用脂質(zhì)體轉(zhuǎn)染法一级毛片a人爱免费视频性色、MTT實(shí)驗(yàn)、腎包膜下移植法www.youjizz.com自拍、流式細(xì)胞儀和DNA梯度試驗(yàn)檢測新耐藥相關(guān)分子DARPP-32對細(xì)胞（SGC7901视色电影、SGC7901/VCR、對阿霉素耐藥的胃癌細(xì)胞SGC7901/ADR）多藥耐藥表型的影響你懂的视频欧美；利用脂質(zhì)體轉(zhuǎn)染法和MTT實(shí)驗(yàn)檢測下調(diào)ZNRD1對DARPP-32介導(dǎo)的胃癌多藥耐藥的調(diào)控作用√α大片。

Methods: Using the ELISA and RT-PCR to detect Rotavirus and astrovirus respectively in stool of the 117 in-patient children diagnosed of enteritis in 2003. The astrovirus positive specimens tested by RT-PCR firstly were serotyped by RT-PCR secondly using the serotype-specific primers, the epidemiological and clinical data including age, season distribution, chief symptoms were compared and analysed.

分別對2003年收集的我院117例腹瀉患兒運(yùn)用ELISA法檢測輪狀病毒及用RT-PCR法檢測星狀病毒，并應(yīng)用兩次擴(kuò)增法對星狀病毒進(jìn)行分型免费观看欧美一级片，并對其臨床及流行性學(xué)資料進(jìn)行統(tǒng)計(jì)學(xué)分析本道无码黄色视频在线播放，包括患兒年齡欧美精品一羞羞，感染季節(jié)及主要臨床癥狀等。