Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同濃度的渥曼青霉素作用于人類髓細胞白血病細胞K562很很操狠狠干很很日，采用MTT法檢測細胞增殖活性先锋影音无码资源，單細胞凝膠電泳技術檢測細胞DNA損傷形成的"彗星"樣拖尾現(xiàn)象欧老妇色XXXX欧美老妇性，Annexin V FITC/PI雙標法檢測細胞凋亡欧美福利网站一区，Western blotting在线视频播放A片、RT-PCR檢測渥曼青霉素作用K562細胞后總Akt和磷酸化Akt以及NF-κB基因及蛋白表達水平的變化有没有网站免费播放黄色一级片。

METHODS K562 cells were cultured for 5 days with different mediums of Tortois plastron, Radix Astragali, Radix Astragalus membranaceus and Radix Codonopsitis pilosula.γ-globin mRNA was quantitated by RT-PCR.

以K562細胞為體外模型，應用熒光定量RT-PCR技術研究藥物誘導后γ-珠蛋白基因mRNA表達水平的變化老女偷偷伦偷偷啪。

AIM To explore and verify the possibility of ap- plica tion of RT-PCR for diagnosis of hemorrhagic fever with renal syndrome, and to study the variations of hantaviruses.

目的 探討RT-PCR用于HFRS臨床標本中病毒基因檢測的若干影響因素及西安地區(qū)近年主要流行漢坦病毒毒株的分子流行病學特點日韩乱码一区二区三区。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

為研究小麥在不同時期脫水素基因的表達情況和生物學功能及抗體制備，以小麥幼芽為材料亚州黄色网站中文字幕，經(jīng)干旱脅迫處理后在线网址你懂，提取總RNA，通過RT-PCR得到小麥類脫水素基因片段(WZY1-1)800凹凸视频在线观看网址，再連接至克隆載體PUCM-T免费看黄网站AV无码app下载，并成功構建重組表達質粒PET-32a-wzy1-1，將陽性重組質粒轉化于受體菌BL21(DE3)感受態(tài)細胞中69人人操人人爱，經(jīng)IPTG誘導表達欧美孕交视频，進行表達產(chǎn)物的聚丙烯酰胺凝膠電泳檢測。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

從美洲商陸葉片中通過異硫氰酸胍法提取出了總RNA人妻丝袜中文字幕三区日韩，經(jīng)RT-PCR擴增出缺失突變型抗病毒蛋白PAP基因黄色美女操，將該基因克隆于分泌型真核表達載體pPIC9K，然后導入畢赤酵母菌株GS115細胞午夜麻豆国产精品视频。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

結果表明99riAV1国产精品视频，該分離病毒無血凝性，在瓊脂擴散試驗中能與抗IBDV特異性血清出現(xiàn)1條清晰的白色沉淀線亚州一二区；人工感染28日齡雛雞出現(xiàn)與臨床一致的病變亚洲精品视频你懂，并回收到病毒；應用針對IBDVVP3基因的特異性引物進行RT-PCR最新黄色视频在线播放，能擴增到長度為1041bp的特異性目的片段；序列分析發(fā)現(xiàn)分離毒VP3基因與IBDV超強毒和經(jīng)典毒株的核苷酸同源性分別為97.7%~98.2%和95.2%欧美精品A∨欧洲精品。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料與方法：成熟雄鼠麻醉后雙側睪丸注射經(jīng)脂質體DOSPER包裹的HBV質粒妇女人人操人人干，手術后雄鼠與超排雌鼠合籠交配A片精品欧美一区二区在线观看，用熒光原位雜變(Fluorescence in situ hybridization，F(xiàn)ISH)分別檢測單細胞胚和二細胞胚間期核中HBV DNA的存在與復制欧美成性互相，用逆轉錄聚含酶鏈反應(Reverse transcriptase-polymerase chain reaction亚洲综合美女在线精品p，RT-PCR)和免疫熒光方法檢測HBV基因在二細胞胚胎中的表達。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位雜交色色网五月、免疫組織化學和RT-PCR等方法丁香五月综合婷婷激情，檢測了PG轉運載體PGT、代謝酶15-PGDH和CBR1在小鼠早期妊娠子宮中的表達性爱不卡视频网站，并利用假孕美女被男人桶岀白浆网站、延遲著床及激活、人工蛻膜化国产精品JIZZJIZZ、性腺類固醇激素處理等模型黄色一级视频在线观看，研究了它們在小鼠子宮中的表達與調(diào)節(jié)。

METHODS:After the treatment of TRAIL or LPS at different doses,we tested the nuclear translocation of NF-κB by cell immunohistochemical staining and electrophoretic mobility shift assay,and evaluated the level of IκB by RT-PCR under pyrrolidine dithiocarbamatetreatment.

當不同濃度的TRAIL和LPS作用于細胞后女性毛片A，我們通過細胞免疫組化染色和凝膠電泳遷移試驗來檢測NF-κB核轉位的情況成年人黄色视频网站。并通過RT-PCR的方法粗略評定二硫代氨基甲酸吡咯烷對抑制蛋白IκB的影響。

Canine distemper virus from samples of dead mink, fox, racoon dog and tiger from different areas was detected by RT-PCR technology.

利用RT-PCR技術檢測了來自不同地區(qū)水貂白白色在线免费观看、狐貍亚洲人人插、貉子和老虎病料中是否存在犬瘟熱病毒。