Making use of RT-PCR to examine the expression of c-fos and c-myc semiquantitatively which is controlling gene of VSMC.

采用 RT-PCR 法半定量檢測(cè) VSMC 的調(diào)控基因c-fos 和 c-myc 表達(dá)的改變規(guī)律国产精品对白交换。

According to the published genomic sequence of TMV ,five sets of PCR primers were designed and five nucleotide segments were amplified by RT-PCR from the total RNA of the TMV infected Rehmannia glutinosa leaves.

根據(jù)已發(fā)表的TMV基因組的核苷酸序列設(shè)計(jì)5對(duì)簡(jiǎn)并引物欧美性一爱398AⅤ视频，以感染TMV的地黃葉片總RNA為模板，經(jīng)RT-PCR擴(kuò)增獲得TMV-RH的5個(gè)核苷酸片段的cDNA国产黄A片免费网站。

If you will be running RT 3.0.x or earlier under FastCGI, you need support for setgid perl scripts.

如果您要在 FastCGI 下運(yùn)行 RT 3.0.x 或者更早插女人视频，您需要 setgid 的 perl 腳本的支持。

A single faecal RT-PCR result on its own is meaningless: if the cat is intermittently shedding FCoV, by the day following sampling the cat may have changed from being a shedder to a non-shedder or vice versa.

單一次的糞便RT-PCR結(jié)果是毫無(wú)意義的：如果貓咪是間歇性的排出FCoV国产午夜精品视频一区二区三区，從貓咪身上取樣的隔天也許會(huì)從會(huì)散播變成為不會(huì)散播欧美精品黑人白妞大战，反之亦然。

This study was designed to clone the duck FABP gene based on the sequences of chicken and mammal FABP gene by comparative genome and bioinformatics approaches; to investigate the expression characterization of FABP genes by RT-PCR; to detect the polymorphisms of FABP genes by DNA sequencing, PCR-SSCP methods, and to investigate the effects of FABP genes polymorphisms on growth and body composition traits in Kunshan sheldrake, Cherry Valley Meat duck and so on.

本研究以雞影音先锋女人av鲁资源网、哺乳動(dòng)物FABP基因序列為基礎(chǔ)黄色黄片一个人www，通過(guò)比較基因組學(xué)和生物信息學(xué)等方法克隆鴨的FABP基因；采用RT-PCR方法研究FABP基因的組織表達(dá)特性看男女搞。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外国产自慰资源，培養(yǎng)人腦膠質(zhì)瘤U251細(xì)胞，進(jìn)行細(xì)胞總RNA抽提高潮黄网站色在线观看免费，運(yùn)用RT-PCR方法進(jìn)行TfR基因的完整編碼區(qū)外顯子的擴(kuò)增欧美嘿咻嘿咻视频播放，經(jīng)過(guò)酶切、連接后構(gòu)建pCDNA3.1-TfR的表達(dá)質(zhì)粒欧美在线播放中文字幕，鑒定正確后按脂質(zhì)體轉(zhuǎn)染方法轉(zhuǎn)染人膠質(zhì)瘤U251細(xì)胞色色五，使其過(guò)量表達(dá)，通過(guò)pEGFPN1-TfR檢測(cè)其瞬時(shí)轉(zhuǎn)染的效率久久国产香蕉毛片一级；用G418對(duì)穩(wěn)定表達(dá)pCDNA3.1-TfR的細(xì)胞進(jìn)行篩選97青青操在线，挑選陽(yáng)性細(xì)胞克隆，運(yùn)用Western blotwww.sanjipian.vom、流式細(xì)胞檢測(cè)技術(shù)和細(xì)胞免疫化學(xué)以及mRNA半定量的方法檢測(cè)TfR在轉(zhuǎn)染前后的表達(dá)水平国产99久久久国产精品电影免费，鑒定轉(zhuǎn)染的效果。

RT-PCR discovered that BcMF14p was expressed prominently in flower buds, flower and silique, but no expression in vegetative organs, this indicated that BcMF14p may be involved in the signal transduction during reproduction development of B. campestris var. purpurea.

對(duì)該信號(hào)分子的表達(dá)進(jìn)行RT-PCR分析色夜在线，結(jié)果顯示BcMF14p只在紫菜薹花蕾狠起插、花欧美性视频在线、角果等生殖器管中表達(dá)www.韩日欧黄色片网站.com，在莖国产亚洲AⅤ人片在线观看、葉等營(yíng)養(yǎng)器官中不表達(dá)，說(shuō)明BcMF14p可能參與紫菜薹生殖發(fā)育過(guò)程中的信號(hào)傳導(dǎo)国产av专区。

RT-PCR results indicate that gene is expressed only in the silk gland in all the tissues tested.

RT-PCR表明該基因僅在本文所檢測(cè)的家蠶絲腺中表達(dá)偷拍偷窥一区二区综合。

RT-PCR detection and analysis of gene chip data showed that silkworm mago nashi gene expressed in all the stages detected during silkworm development, including embryo, larva, pupa and moth. However, higher expression could be visualized in gonads and silk gland compaired with other tissues.

RT—PCR檢測(cè)和組織芯片數(shù)據(jù)分析結(jié)果表明，家蠶mago nashi基因在胚胎国产激情图区、幼蟲(chóng)免费人成视频在饯观看网站、蛹和蛾期都有表達(dá)，在5齡第3天幼蟲(chóng)的各組織中也均有表達(dá)日韩欧美精品视频在线观看，但在生殖腺和絲腺中的表達(dá)量高于其它組織国产a第⑩页无毒。

Using RT-PCR analysis, RGS7 was expressed from fertilization to 96 hours post-fertilization of Zebrafish. Expression of RGS7 was found in brain、aorta ventralis超碰在线入口、intermediate cell mass and somite by whole-mount in situ hybridization.

因此久久精品国产福利，利用RT-PCR以及全胚體原位雜合技術(shù)，結(jié)果發(fā)現(xiàn)在受精后0~96小時(shí)的斑馬魚(yú)胚胎中皆可偵測(cè)到RGS7的表現(xiàn)免费视频性综合，其表現(xiàn)的位置是在斑馬魚(yú)胚胎的腦部欧美亚洲国产另类、腹側(cè)主動(dòng)脈、中期細(xì)胞團(tuán)及體節(jié)等組織部位湖北一级黄片。