Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根據(jù)NCBI公布的大葉補(bǔ)血草NHX1基因序列設(shè)計(jì)特異性引物爱爱免费的小视频在，經(jīng)RT-PCR方法克隆得到大葉補(bǔ)血草液泡膜Na/H逆向轉(zhuǎn)運(yùn)蛋白基因NHX1色站在线直播，構(gòu)建植物融合表達(dá)載體pCAMBIA1301-NHX1黄片一级片免费看，重組質(zhì)粒通過農(nóng)桿菌介導(dǎo)轉(zhuǎn)化番茄欧美肏穴网，經(jīng)PCR和RT-PCR鑒定日韩特黄Av毛片免费播放，獲得轉(zhuǎn)基因番茄植株色惰日本人哺乳奶水视频网1。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

為探討Dynein與卵母細(xì)胞減數(shù)分裂錯(cuò)誤形成的關(guān)系，采用小鼠卵母細(xì)胞體外培養(yǎng)成熟技術(shù)和半定量RT-PCR方法国产女人A片免费视频，分別檢測(cè)Dynein抑制劑-原釩酸鈉對(duì)卵母細(xì)胞成熟率和cyclin B1基因轉(zhuǎn)錄水平的影響欧美成电影网。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后无码专区视频一区古典武侠，用免疫熒光的方法檢測(cè)β-catenin的表達(dá)和在細(xì)胞上定位的變化久久久久亚洲色欲AV无码裤子；用半定量RT-PCR檢測(cè)β-catenin mRNA表達(dá)的變化；用質(zhì)粒轉(zhuǎn)染的方法檢測(cè)熒光素酶報(bào)告基因Tcf轉(zhuǎn)錄的情況99插插插；用半定量RT-PCR檢測(cè)Wnt/β-catenin通路下游基因COX-2国产精品午夜激爽毛片，cyclinD1， c-fos日本视频中文字幕在线， c-Jun mRNA表達(dá)的情況欧美一级欧美三级视频在线播破处女；用免疫共沉淀的方法檢測(cè)β-catenin和E-Cadherin的結(jié)合情況。

In order to establish the detection of Hemagglutinating Encephalomyelitis Virus by RT- PCR, a pair of oligonucleotide primers appropriate for PCR amplication was selected based on the HE gene and S gene of HEV.

為了建立快速檢測(cè)豬血凝性腦脊髓炎病毒的RT-PCR方法www.欧美专线电影，根據(jù)GenBank中HEV的HE基因和S基因設(shè)計(jì)了1對(duì)引物在线观看免费播放国产一区在线，在優(yōu)化RT-PCR反應(yīng)條件的基礎(chǔ)上，成功的擴(kuò)增出323bp的特異性條帶偷拍久久丝袜免费视频。

METHODS: Sixty two cases of specimens collected in operation were studied by in situ RT PCR, of which 50 cases were hemangioma: 26 cases proliferating hemangioma and 24 cases involuting hemangioma, and 12 cases were venous vascular malformation. The expression of Ang2 mRNA and Tie2 mRNA in hemangiomas and venous vascular malformation were detected by in situ RT PCR.

采用原位RT PCR方法在线可以直接观看的黄色视频。，對(duì)臨床收集的62例手術(shù)切除的先天性皮膚血管標(biāo)本（其中增生期血管瘤26例，消退期血管瘤24例色先锋玖玖av资源部每次更新，靜脈型血管畸形12例）進(jìn)行Ang2 mRNA最新国产乱伦视频， Tie2 mRNA表達(dá)水平的檢測(cè)。

Esults LVSV and RVSV determined by RT-3DE were (15.90±10.62) ml and (12.42±4.69) ml respectively, which were shown good correlation with those determined by MRI [(16.56±10.64)ml and (13.96±7.96) ml]. The r value was 0.98, and 0.75, respectively. LVSV and RVEV determined by RT-3DE were (12.67±10.10) ml and (11.83±12.02) ml respectively, which were well correlated with MASE (8.17±1.92)mm and TASE (11.04±1.06) mm determined with ME. The r value was 0.86 and 0.88 respectively.

T-3DE測(cè)定左室每搏量(12.67±10.10)ml和ME測(cè)定二尖瓣瓣環(huán)收縮期平均位移(8.17±1.92)mm之間的相關(guān)性顯著日本熟妇裸交ⅩXX视频全过程，相關(guān)系數(shù)r=0.86(P.001)8050午夜级毛片一级；RT-3DE測(cè)右室每搏量(11.83±12.02)ml和ME測(cè)定三尖瓣瓣環(huán)收縮期位移(11.04±3.06)mm兩者亦存在顯著正相關(guān)激情中文字幕图片区小说区，相關(guān)系數(shù)r=0.88(P.001)。

CEVd is a non-polyadenylated RNA viroid,but there is a fragment rich in A in CEVd sequence.On the bases of uniplex RT-PCR, a multiplex RT-PCR was developed to detect CTV,CEVd and CTLV simultaneously.

實(shí)驗(yàn)過程中国产老太毛多女人视频，發(fā)現(xiàn)不具有ploy_加尾的環(huán)狀類病毒CEVd97视频人人摸，同樣可以用oligo反轉(zhuǎn)錄的RT-PCR檢測(cè)出來，通過測(cè)序分析爱草免费人妻，其同源性在96%以上狼狠亚洲综合狼狼久久，并發(fā)現(xiàn)在CEVd序列中有一個(gè)富含A的區(qū)段。

Objective To establish a competitive RT-PCR method for quantitative determination of IFN-γ mRNA and IL-4 mRNA and explore the optimal inductive condition in vitro. Methods IFN-γ mRNA and IL-4 mRNA in PBMC induced by PMA and calcium ionophore of a healthy volunteer and in unstimulated PBMC of 11 HVs and 13 asthma patients were determined quantitatively with the established competitive RT-PCR method using self-prepared internal standard as competitive templets.

Subject words 】 Competitive templet乱论强奸欧美； IFN-γ国产成人中文激情小说在线观看网站； IL-4； mRNA性爱噜噜； RT-PCR 檢測(cè)細(xì)胞因子最早使用生物學(xué)或免疫學(xué)測(cè)定法玖玖玖在线视频免费播放，這兩類檢測(cè)可反映某種細(xì)胞因子分泌、吸附wwwcom黄色网站在线观看、消耗和降解后的凈含量〔1〕亚洲黄色片免费看，但不能為細(xì)胞因子產(chǎn)生過程中基因轉(zhuǎn)錄、翻譯及翻譯后調(diào)控機(jī)制提供必要的信息日韩国产欧美在线一区二区三区，也不能反映細(xì)胞或組織內(nèi)細(xì)胞因子基因表達(dá)水平久久精品免费视频小说。

The same 20 viruses have been previoushy typed using serotyping method resulting that only 11 could be typed successfully, showing a high consistency with that of RT-PCR-RFLP method and a 30% lower typing efficiency than the latter.

此20個(gè)毒株曾用血清學(xué)方法分型，僅11株分型成功国产精品一二三四五，與RT-PCR-RFLP法結(jié)果符合色色一区二区三区。分型成功率RT-PCR-RFLP法比血清法高30%。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus18以下勿进色禁网站免费看， BFV)中國株BFV3026原病毒DNA為材料黄色片一级a片，構(gòu)建R區(qū)系列缺失質(zhì)粒，通過對(duì)其轉(zhuǎn)染細(xì)胞中RT水平及對(duì)缺失質(zhì)粒與luc報(bào)告質(zhì)粒共轉(zhuǎn)染細(xì)胞中螢火蟲熒光素酶活性的測(cè)定亚洲色图欧美性爱二区，確立U5區(qū)對(duì)于BFV3026兩類啟動(dòng)子LTR和IP均具有負(fù)調(diào)控作用欧美精品一二三四区0；同時(shí)將帶有不同R區(qū)的BFV3026結(jié)構(gòu)基因片段克隆于異源啟動(dòng)子CMV之下，通過對(duì)其轉(zhuǎn)染細(xì)胞293T中RT酶活性的測(cè)定国产迷奸免费看，確立R區(qū)對(duì)于病毒結(jié)構(gòu)基因pol的表達(dá)具有一定的調(diào)節(jié)作用韩国三级HD中文字幕叫床，并將其功能區(qū)域初步界定在R區(qū)5′端100bp內(nèi)。