Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

參照GenBank登錄的綿羊凝乳酶原前體cDNA序列設(shè)計(jì)引物，以新生5d的西農(nóng)薩能羊皺胃組織總RNA為模版加日韩加勒比一本无码精品，通過(guò)RT-PCR方法獲得凝乳酶原前體cDNA，克隆測(cè)序后進(jìn)行序列比對(duì)。

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

運(yùn)用RT-PCR技術(shù)從馬鹿布氏桿菌分離株中擴(kuò)增出Omp25基因日韩无码黄片，將其克隆入pMD18-T中進(jìn)行核苷酸序列測(cè)定和遺傳變異分析，并將目的基因亞克隆到大腸桿菌表達(dá)載體pET28a中誘導(dǎo)表達(dá)日本熟妇高清色本在线婷婷。

RT-PCR analysis showed that the ZmASK1 expression was induced by mannitol, salt and abscisic acid.

半定量RT-PCR分析表明色色91，ZmASK1可以被甘露醇、高鹽和脫落酸誘導(dǎo)日日摸日日。

RT-PCR analysis showed that the ZmPti1 expression was induced by salicylic acid, low-temperature, mannitol and salt but not by abscisic acid.

半定量RT-PCR分析結(jié)果顯示ZmPtil基因的表達(dá)可以被水楊酸欧美在线a片播放97视频、低溫天堂网黄色网站在线观看、甘露醇和鹽所誘導(dǎo)，但是不被脫落酸所誘導(dǎo)a一级黄色网。

Then, two primers were designed from it, the full length cDNA of malate dehydrogenase gene in Chinese cabbage was gained through RT-PCR amplifying and sequencing and entered GenBank as accession number FJ208590, which is 1 209 bp length and coded 403 amino acids.

然后設(shè)計(jì)引物毛片一级不卡免费，經(jīng)RT-PCR擴(kuò)增、測(cè)序曰本一级毛片免费高清直播，獲得了大白菜蘋(píng)果酸脫氫酶基因的cDNA全長(zhǎng)序列亚洲毛片一级无码网站，并已將其登錄到GenBank，登錄號(hào)為：FJ208590亚洲一级精品A片免费视频，該cDNA全長(zhǎng)1 209 bp正在播放国产一区二区中文，編碼403個(gè)氨基酸。

The competitive RT-PCR used in this study overcomes the limitations of β-actin method.

研究中選用競(jìng)爭(zhēng)RT-PCR方法克服了以往使用β-actin的局限性一级A片免费天天噜。

K562 cell cultivated in suspension and adhesively cultivated with msc were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of k562 cell was investigated,the mdr1 gene expression was determined by rt-pcr.

對(duì)懸浮培養(yǎng)和與msc黏附培養(yǎng)的k562細(xì)胞繪制細(xì)胞生長(zhǎng)曲線(xiàn)色色五月黄片免费看；用流式細(xì)胞術(shù)測(cè)定細(xì)胞周期并觀察化學(xué)藥物對(duì)細(xì)胞存活率和凋亡的影響；用rt-pcr技術(shù)檢測(cè)mdr1基因表達(dá)欧美亚洲日韩国产精品第6页。

K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of K562 cell was investigated, the MDR1 gene expression was determined by RT-PCR.

葉懸浮培養(yǎng)和與MSC黏附培養(yǎng)的K562細(xì)胞繪制細(xì)胞生長(zhǎng)曲線(xiàn)日韩精品短片影院在线播放视频免费；用流式細(xì)胞術(shù)測(cè)定細(xì)胞周期并觀察化學(xué)藥物對(duì)細(xì)胞存活率和凋亡的影響；用RT-PCR技術(shù)檢測(cè)MDR1基因表達(dá)日夲一级片。

A is the central administrative domain of RT; it keeps things organized.

隊(duì)列是RT的首要的管理的領(lǐng)域你懂得手机在线播放；它使得事情有條理。

The level of GR mRNA expression in hepatic cytosol were determined by RT-PCR. Plasma adrenocorticotrophic hormone and glucocorticoid concentrations were determined by the radioimmunoassay.

以[^3H]地塞米松為配體国产成长精品视频，用一點(diǎn)分析法測(cè)腦和肝胞液GR結(jié)合活性在线儿播放你懂的、半定量RT—PCR方法測(cè)肝胞液GR mRNA水平、放免法測(cè)血漿促腎上腺皮質(zhì)激素和皮質(zhì)酮濃度欧美行做久久久久久久。